Title:
METHOD FOR DETECTING TARGET NUCLEIC ACID
Document Type and Number:
WIPO Patent Application WO/2013/039228
Kind Code:
A1
Abstract:
The disclosure provides a method for detecting a target nucleic acid whereby efficient probe hybridization is possible. To this end, a target nucleic acid is amplified using: a first primer containing a first recognition sequence that recognizes a first base sequence in the target nucleic acid and a tag sequence complementary to a detection probe preassociated with the target nucleic acid, and having a link site capable of suppressing or stopping a DNA polymerase reaction between this tag sequence and this first recognition sequence; and a second primer containing a second recognition sequence that recognizes a second base sequence in the target nucleic acid; the amplified fragment and the detection probe are brought into contact to enable hybridization, and the hybridized product is detected.
Inventors:
NIWA KOUSUKE (JP)
HIROTA TOSHIKAZU (JP)
KAWASE MITSUO (JP)
HIROTA TOSHIKAZU (JP)
KAWASE MITSUO (JP)
Application Number:
PCT/JP2012/073710
Publication Date:
March 21, 2013
Filing Date:
September 14, 2012
Export Citation:
Assignee:
NGK INSULATORS LTD (JP)
NIWA KOUSUKE (JP)
HIROTA TOSHIKAZU (JP)
KAWASE MITSUO (JP)
NIWA KOUSUKE (JP)
HIROTA TOSHIKAZU (JP)
KAWASE MITSUO (JP)
International Classes:
C12N15/09; C12Q1/68; G01N33/543
Domestic Patent References:
| WO2004039825A2 | 2004-05-13 | |||
| WO2003050305A1 | 2003-06-19 | |||
| WO1997024455A2 | 1997-07-10 | |||
| WO1992008728A1 | 1992-05-29 | |||
| WO2009034842A1 | 2009-03-19 |
Foreign References:
| JP2010014507A | 2010-01-21 | |||
| JP2006211982A | 2006-08-17 | |||
| JP2006101844A | 2006-04-20 | |||
| JP2003108126A | 2003-04-11 | |||
| JP2008306941A | 2008-12-25 | |||
| JP2009000024A | 2009-01-08 | |||
| JP2003075305A | 2003-03-12 |
Other References:
URDEA, MICKEY S. ET AL.: "A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes.", NUCLEIC ACIDS RESEARCH, vol. 16, no. 11, 1988, pages 4937 - 4956, XP001080119
ANALYTICAL BIOCHEMISTRY, vol. 364, 2007, pages 78 - 85
PROCEEDINGS OF THE 14TH ANNUAL MEETING OF THE ASSOCIATION FOR THE RAPID METHOD AND AUTOMATION IN MICROBIOLOGY (ARMAM, pages 45 - 50
H. YOSHIDA; A. SUYAMA: "Solution to 3-SAT by breadth first search", DIMACS, vol. 54, 2000, pages 9 - 20
See also references of EP 2762562A4
ANALYTICAL BIOCHEMISTRY, vol. 364, 2007, pages 78 - 85
PROCEEDINGS OF THE 14TH ANNUAL MEETING OF THE ASSOCIATION FOR THE RAPID METHOD AND AUTOMATION IN MICROBIOLOGY (ARMAM, pages 45 - 50
H. YOSHIDA; A. SUYAMA: "Solution to 3-SAT by breadth first search", DIMACS, vol. 54, 2000, pages 9 - 20
See also references of EP 2762562A4
Attorney, Agent or Firm:
KAI-U PATENT LAW FIRM (JP)
Patent business corporation KAI-U Patent Law Firm (JP)
Patent business corporation KAI-U Patent Law Firm (JP)
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