Title:
METHOD FOR DETECTING TARGET NUCLEIC ACID ON BASIS OF ELECTROCHEMICAL REAL-TIME POLYMERASE CHAIN REACTION USING APTAMER-MODIFIED PRIMER
Document Type and Number:
WIPO Patent Application WO/2019/151756
Kind Code:
A1
Abstract:
The present invention relates to a method for detecting a target nucleic acid by an electrochemical real-time polymerase chain reaction using a primer including an aptamer base sequence of a signaling material that generates an electrochemical signal. A method for detecting a target nucleic acid on the basis of an electrochemical real-time polymerase chain reaction using an aptamer-modified primer according to the present invention can establish an electrochemical nucleic acid detection system that is inexpensive and which allows the easy miniaturization of the analysis apparatus, thereby finding applications in the in-situ diagnosis of various genetic diseases and infectious diseases.
Inventors:
PARK HYUN GYU (KR)
LEE JOON YOUNG (KR)
KIM SOEUN (KR)
LEE JOON YOUNG (KR)
KIM SOEUN (KR)
Application Number:
PCT/KR2019/001256
Publication Date:
August 08, 2019
Filing Date:
January 30, 2019
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
BIONANO HEALTH GUARD RES CENTER (KR)
BIONANO HEALTH GUARD RES CENTER (KR)
International Classes:
C12Q1/6876; C12Q1/6886
Foreign References:
| KR101454511B1 | 2014-10-23 | |||
| KR101319492B1 | 2013-10-17 |
Other References:
ZHOU, WENHU: "Metal sensing by DNA", CHEMICAL REVIEWS, vol. 117, no. 12, 9 June 2017 (2017-06-09), pages 8272 - 8325, XP055628485
CAO, ZHI-JUAN: "Highly sensitive chemiluminescence technology for protein detection using aptamer-based rolling circle amplification platform", JOURNAL OF PHARMACEUTICAL ANALYSIS, vol. 1, no. 3, August 2011 (2011-08-01), pages 159 - 165, XP055628490
ZHU, YINGYUE ET AL.: "Mercury-DNA interaction based detection of mercury ions by DNA amplification with high sensitivity and selectivity", FOOD AND AGRICULTURAL IMMUNOLOGY, vol. 26, no. 4, 9 December 2014 (2014-12-09) - 2015, pages 512 - 520, XP055628492
CAO, ZHI-JUAN: "Highly sensitive chemiluminescence technology for protein detection using aptamer-based rolling circle amplification platform", JOURNAL OF PHARMACEUTICAL ANALYSIS, vol. 1, no. 3, August 2011 (2011-08-01), pages 159 - 165, XP055628490
ZHU, YINGYUE ET AL.: "Mercury-DNA interaction based detection of mercury ions by DNA amplification with high sensitivity and selectivity", FOOD AND AGRICULTURAL IMMUNOLOGY, vol. 26, no. 4, 9 December 2014 (2014-12-09) - 2015, pages 512 - 520, XP055628492
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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