Title:
METHOD FOR DETECTING TOXIN-PRODUCING CLOSTRIDIUM DIFFICILE
Document Type and Number:
WIPO Patent Application WO/2013/031973
Kind Code:
A1
Abstract:
Provided are an oligonucleotide allowing toxin-producing clostridium difficile to be specifically detected with high sensitivity, and a method of detecting toxin-producing clostridium difficile using same. 1) A primer pair including an oligonucleotide consisting of a base sequence indicated by SEQ ID. NO. 1 and an oligonucleotide consisting of a base sequence indicated by SEQ ID. NO. 2, or a primer pair including complementary base sequences corresponding thereto. 2) A primer pair including an oligonucleotide consisting of a base sequence indicated by SEQ ID. NO. 4 and an oligonucleotide consisting of a base sequence indicated by SEQ ID. NO. 5, or a primer pair including complementary base sequences corresponding thereto.
Inventors:
KUBOTA HIROYUKI (JP)
MAKINO HIROSHI (JP)
SAKAI TAKAFUMI (JP)
ISHIKAWA EIJI (JP)
OISHI KENJI (JP)
MAKINO HIROSHI (JP)
SAKAI TAKAFUMI (JP)
ISHIKAWA EIJI (JP)
OISHI KENJI (JP)
Application Number:
PCT/JP2012/072219
Publication Date:
March 07, 2013
Filing Date:
August 31, 2012
Export Citation:
Assignee:
YAKULT HONSHA KK (JP)
KUBOTA HIROYUKI (JP)
MAKINO HIROSHI (JP)
SAKAI TAKAFUMI (JP)
ISHIKAWA EIJI (JP)
OISHI KENJI (JP)
KUBOTA HIROYUKI (JP)
MAKINO HIROSHI (JP)
SAKAI TAKAFUMI (JP)
ISHIKAWA EIJI (JP)
OISHI KENJI (JP)
International Classes:
C12Q1/68; C12N15/09; C12Q1/04; C12Q1/06
Domestic Patent References:
| WO2009061752A1 | 2009-05-14 |
Foreign References:
| JP2010537648A | 2010-12-09 | |||
| JP2003164282A | 2003-06-10 | |||
| JP2003164282A | 2003-06-10 |
Other References:
DE BOER R.F. ET AL.: "Evaluation of a rapid molecular screening approach for the detection of toxigenic Clostridium difficile in general and subsequent identification of the tcdC A117 mutation in human stools", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 83, 2010, pages 59 - 65, XP027265471
BELANGER S. D. ET AL.: "Rapid Detection of Clostridium difficile in Feces by Real-Time PCR", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 41, no. 2, 2003, pages 730 - 734, XP002561409
KAZUNORI MATSUDA ET AL.: "Sensitive Quantification of Clostridium difficile in the Stool Samples of Healthy Adults by Quantitative RT-PCR", JAPANESE JOURNAL OF BACTERIOLOGY, vol. 63, no. 1, 2008, pages 166, XP008173106
RINTTILA T. ET AL.: "Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR", JOURNAL OF APPLIED MICROBIOLOGY, vol. 97, 2004, pages 1166 - 1177, XP008128526
BELANGER, S. D; M. BOISSINOT; N. CLAIROUX; F. J. PICARD; M. G. BERGERON, J. CLIN.MICROBIOL, vol. 41, 2003, pages 730 - 4
HOUSER, B. A.; A. L. HATTEL; B. M. JAYARAO., FOODBORNE PATHOG. DIS., vol. 7, 2010, pages 719 - 26
SLOAN, L. M.; B. J. DURESKO; D. R. GUSTAFSON; J. E. ROSENBLATT., J. CLIN. MICROBIOL., vol. 46, 2008, pages 1996 - 2001
MATSUKI, T.; K. WATANABE; J. FUJIMOTO; Y. KADO; T. TAKADA; K. MATSUMOTO; R. TANAKA: "Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria", APPL. ENVIRON. MICROBIOL., vol. 70, 2004, pages 167 - 173, XP002431255, DOI: doi:10.1128/AEM.70.1.167-173.2004
BELANGER S. D. ET AL.: "Rapid Detection of Clostridium difficile in Feces by Real-Time PCR", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 41, no. 2, 2003, pages 730 - 734, XP002561409
KAZUNORI MATSUDA ET AL.: "Sensitive Quantification of Clostridium difficile in the Stool Samples of Healthy Adults by Quantitative RT-PCR", JAPANESE JOURNAL OF BACTERIOLOGY, vol. 63, no. 1, 2008, pages 166, XP008173106
RINTTILA T. ET AL.: "Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR", JOURNAL OF APPLIED MICROBIOLOGY, vol. 97, 2004, pages 1166 - 1177, XP008128526
BELANGER, S. D; M. BOISSINOT; N. CLAIROUX; F. J. PICARD; M. G. BERGERON, J. CLIN.MICROBIOL, vol. 41, 2003, pages 730 - 4
HOUSER, B. A.; A. L. HATTEL; B. M. JAYARAO., FOODBORNE PATHOG. DIS., vol. 7, 2010, pages 719 - 26
SLOAN, L. M.; B. J. DURESKO; D. R. GUSTAFSON; J. E. ROSENBLATT., J. CLIN. MICROBIOL., vol. 46, 2008, pages 1996 - 2001
MATSUKI, T.; K. WATANABE; J. FUJIMOTO; Y. KADO; T. TAKADA; K. MATSUMOTO; R. TANAKA: "Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria", APPL. ENVIRON. MICROBIOL., vol. 70, 2004, pages 167 - 173, XP002431255, DOI: doi:10.1128/AEM.70.1.167-173.2004
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
Patent business corporation Alga patent firm (JP)
Patent business corporation Alga patent firm (JP)
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