METHOD FOR DETECTING TRACE NUCLEIC ACIDS IN VARIOUS MIXED NUCLEIC ACIDS

Title:

METHOD FOR DETECTING TRACE NUCLEIC ACIDS IN VARIOUS MIXED NUCLEIC ACIDS

Document Type and Number:

WIPO Patent Application WO/2021/254009

Kind Code:

A1

Abstract:

Disclosed in the present invention is a method for detecting trace nucleic acids by means of nucleic acid cyclization and selective nucleic acid disruption. The method comprises performing a cyclization treatment on a to-be-detected gene fragment and selectively performing a disruption treatment on a specific circular gene fragment. Different nucleic acid molecules respectively exhibit a circular state and a linear state by means of cyclization and a selective disruption treatment so as to be distinguished, and the technology can be used for identifying specific genotype nucleic acid molecules.

More Like This:

WO/2014/151511	SYSTEMS AND METHODS FOR DETECTION OF GENOMIC COPY NUMBER CHANGES
WO/2020/102594	LOW BINDING SUPPORTS FOR IMPROVED SOLID-PHASE DNA HYBRIDIZATION AND AMPLIFICATION
WO/2007/111924	MOTION RESOLVED MOLECULAR SEQUENCING

Inventors:

LI KAI (CA)
LIAO DUANFANG (CN)
XIAO LI (CN)
ZHANG ANDY (US)
XING CHUNGEN (CN)

Application Number:

PCT/CN2021/091723

Publication Date:

December 23, 2021

Filing Date:

April 30, 2021

Export Citation:

Click for automatic bibliography generation Help

Assignee:

LI KAI (CN)

International Classes:

C12Q1/68

Domestic Patent References:

WO2019117714A1

2019-06-20

Foreign References:

CN111876472A	2020-11-03
CN101421410A	2009-04-29

Other References:

SH LEE ET AL.: "CUT-PCR: CRISPR-mediated, ultrasensitive detection of target DNA using PCR", ONCOGENE, vol. 36, no. 49, 28 August 2017 (2017-08-28), pages 6823 - 6829, XP055779070, DOI: 10.1038/onc.2017.281

Attorney, Agent or Firm:

SUZHOU CREATOR PATENT AND TRADEMARK AGENCY LTD. (CN)

Download PDF:

View/Download PDF PDF Help

Previous Patent: ELECTRONIC DEVICE

Next Patent: HEAT EXCHANGER FLAT TUBE AND HEAT EXCHANGER HAVING SAME