Title:
METHOD FOR DETECTING UNDIFFERENTIATED CELLS
Document Type and Number:
WIPO Patent Application WO/2021/095797
Kind Code:
A1
Abstract:
Provided is a marker gene capable of detecting the persistence/admixture of undifferentiated cells in a differentiated cell population. Undifferentiated cells present in a differentiated cell population are detected by using at least one gene selected from the group consisting of LINC00678 and PRDM14 as an undifferentiation marker. Also provided are a method for detecting undifferentiated cells, a method of use as an undifferentiation marker, and an undifferentiated cell detection kit, and a method for selecting an undifferentiated cell line.
Inventors:
TANIGUCHI HIDEKI (JP)
SEKINE KEISUKE (JP)
YASUI RYOTA (JP)
SEKINE KEISUKE (JP)
YASUI RYOTA (JP)
Application Number:
PCT/JP2020/042209
Publication Date:
May 20, 2021
Filing Date:
November 12, 2020
Export Citation:
Assignee:
PUBLIC UNIV CORP YOKOHAMA CITY UNIV (JP)
EIKEN CHEMICAL (JP)
EIKEN CHEMICAL (JP)
International Classes:
C07K16/18; C12N5/0735; C12N5/09; C12N5/10; C12Q1/6837; C12Q1/6841; C12Q1/6844; C12Q1/686; C12Q1/6862; C12Q1/6869; C12Q1/6872; C12Q1/6876
Foreign References:
| JP2019207002A | 2019-12-05 | |||
| JPS6124348B2 | 1986-06-10 |
Other References:
CHEN, J. ET AL.: "Transcriptome comparison of human neurons generated using induced pluripotent stem cells derived from dental pulp and skin fibroblasts", PLOS ONE, vol. 8, no. 10, 2013, pages 1 - 9, XP002785693
TSUNEYOSHI, N. ET AL.: "PRDM14 suppresses expression of differentiation marker genes in human embryonic stem cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 367, 2008, pages 899 - 905, XP022449891, DOI: 10.1016/j.bbrc.2007.12.189
YUKI HAMADA: "P-03-009 Establishment of safety evaluation method for clinical application of human iPSC hepatoblastoma", THE 17TH CONGRESS OF THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE; MARCH 21-23, 2018, 23 February 2018 (2018-02-23) - 23 March 2018 (2018-03-23), JP, pages 644, XP055663130
TANG, C. ET AL.: "An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-formin", NATURE BIOTECHNOLOGY, vol. 29, no. 9, 14 August 2011 (2011-08-14), pages 829 - 834, XP055067218, DOI: 10.1038/nbt.1947
RAMATHAL, C. ET AL.: "Fate of iPSCs derived from azoospermic and fertile men following xenotransplantation to murine seminiferous tubules", CELL REPORTS, vol. 7, no. 4, 22 May 2014 (2014-05-22), pages 1284 - 1297, XP055823725
WOLFRUM, K. ET AL.: "The large principle of cellular reprogramming: Lost, acquired and retained gene expression in foreskin and amniotic fluid-derived human iPS cells", PLOS ONE, vol. 5, no. 10, 29 October 2010 (2010-10-29), pages e13703, XP055823728
TANO ET AL., PLOS ONE, vol. 27, no. 10, 2014, pages e110496
PLOS ONE, vol. 7, no. 5, 2012, pages e37342
"NCBI", Database accession no. NM 024504.4
NATURE, vol. 499, 2013, pages 481 - 484
CELL REP., vol. 21, 2017, pages 2661 - 2670
MENENDEZ L. ET AL., PROC NATL ACAD SCI USA., vol. 108, no. 48, 2011, pages 19240 - 5
KURODA T. ET AL., PLOS ONE, vol. 7, no. 5, 2012, pages e37342
See also references of EP 4059960A4
TSUNEYOSHI, N. ET AL.: "PRDM14 suppresses expression of differentiation marker genes in human embryonic stem cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 367, 2008, pages 899 - 905, XP022449891, DOI: 10.1016/j.bbrc.2007.12.189
YUKI HAMADA: "P-03-009 Establishment of safety evaluation method for clinical application of human iPSC hepatoblastoma", THE 17TH CONGRESS OF THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE; MARCH 21-23, 2018, 23 February 2018 (2018-02-23) - 23 March 2018 (2018-03-23), JP, pages 644, XP055663130
TANG, C. ET AL.: "An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-formin", NATURE BIOTECHNOLOGY, vol. 29, no. 9, 14 August 2011 (2011-08-14), pages 829 - 834, XP055067218, DOI: 10.1038/nbt.1947
RAMATHAL, C. ET AL.: "Fate of iPSCs derived from azoospermic and fertile men following xenotransplantation to murine seminiferous tubules", CELL REPORTS, vol. 7, no. 4, 22 May 2014 (2014-05-22), pages 1284 - 1297, XP055823725
WOLFRUM, K. ET AL.: "The large principle of cellular reprogramming: Lost, acquired and retained gene expression in foreskin and amniotic fluid-derived human iPS cells", PLOS ONE, vol. 5, no. 10, 29 October 2010 (2010-10-29), pages e13703, XP055823728
TANO ET AL., PLOS ONE, vol. 27, no. 10, 2014, pages e110496
PLOS ONE, vol. 7, no. 5, 2012, pages e37342
"NCBI", Database accession no. NM 024504.4
NATURE, vol. 499, 2013, pages 481 - 484
CELL REP., vol. 21, 2017, pages 2661 - 2670
MENENDEZ L. ET AL., PROC NATL ACAD SCI USA., vol. 108, no. 48, 2011, pages 19240 - 5
KURODA T. ET AL., PLOS ONE, vol. 7, no. 5, 2012, pages e37342
See also references of EP 4059960A4
Attorney, Agent or Firm:
MAYAMA, Setsuko et al. (JP)
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