Title:
METHOD FOR DETECTING VARIANT OF BRASSICA OLERACEA PLANT
Document Type and Number:
WIPO Patent Application WO/2019/035480
Kind Code:
A1
Abstract:
The present invention relates to a method for detecting a heteroploid of a Brassica oleracea plant, the method including carrying out real time PCR using DNA extracted from a sample from a Brassica oleracea plant of interest as a template and using DNA markers respectively specific to at least two chromosomes of a Brassica oleracea plant to obtain a relative difference in amplification levels between the DNA markers, and then detecting chromosomal aneuploidy from the relative difference. According to the present invention, it becomes possible to detect a variant (chromosomal heteroploidy) that may occur in Brassica oleracea varieties simply, accurately and rapidly in a laboratory or the like which has common molecular biological skills in the course of the management of quality of seeds or the study on breeding.
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Inventors:
IZUMIDA ATSUSHI (JP)
SUZUKI TAKAO (JP)
HIRAMOTO TETSUYA (JP)
SUZUKI TAKAO (JP)
HIRAMOTO TETSUYA (JP)
Application Number:
PCT/JP2018/030486
Publication Date:
February 21, 2019
Filing Date:
August 17, 2018
Export Citation:
Assignee:
SAKATA SEED CORP (JP)
International Classes:
C12N15/09; C12Q1/686; C12Q1/6876
Foreign References:
| JP3836451B2 | 2006-10-25 |
Other References:
CHABLE, V. ET AL.: "Aberrant''plants in cauliflower: 2. Aneuploidy and global DNA methy lat ion", EUPHYTICA, vol. 170, 2009, pages 275 - 287, XP019748278
ZIMMERMANN, B. ET AL.: "Novel real-time quantitative PCR test for trisomy 21", CLIN. CHEM., vol. 48, no. 2, 2002, pages 362 - 363, XP002213516
DUDAREWICZ, L. ET AL.: "Molecular methods for rapid detection of aneuploidy", J. APPL. GENET., vol. 46, no. 2, 2005, pages 207 - 215, XP055577114
TREFF, N. R. ET AL.: "Development and validation of an accurate quantitative real-time polymerase chain reaction-based assay for human blastocyst comprehensive chromosomal aneuploidy Screening", FERTIL. STERIL., vol. 97, no. 4, 2012, pages 819 - 824, XP028478554
HASHEMI, M. ET AL.: "A novel multiplex real-time PCR assay using TaqMan MGB probes for rapid detection of trisomy 21", MIDDLE EAST JOURNAL OF SCIENTIFIC RESEARCH, vol. 6, no. 4, 2010, pages 398 - 402, XP055675513, ISSN: 1990-9233
V. RUFFIO-CHABLE ET AL.: "Developmentally ''Aberrant'' plants in F1 hybrids of Brassica oleracea", ISHS ACTA HORTICULTURAE, vol. 539, 2000, pages 89
V. CHABLE ET AL.: "Aberrant'' plants in cauliflower: 2. Aneuploidy and global DNA methylation", EUPHYTICA, vol. 170, 2009, pages 275, XP019748278, DOI: 10.1007/s10681-009-9978-4
N. ROUX ET AL.: "Rapid detection of aneuploidy in Musa using flow cytometry", PLANT CELL REPORT, vol. 21, 2003, pages 483
I. PARKIN ET AL.: "Segmental structure of the Brassica napus genome based on comparative analysis with Arabidopsis thaliana", GENETICS, vol. 171, 2005, pages 765
X. CHENG ET AL.: "Development and genetic mapping of microsatellite markers from genome survey sequences in Brassica napus", THEORETICAL APPLIED GENETICS, vol. 118, 2009, pages 1121, XP019698439
SAIKI RK ET AL., SCIENCE, vol. 230, 1985, pages 1350 - 1354
"Plant PCR Experimental Protocol in Plant Cell Engineering", 1995
See also references of EP 3670657A4
ZIMMERMANN, B. ET AL.: "Novel real-time quantitative PCR test for trisomy 21", CLIN. CHEM., vol. 48, no. 2, 2002, pages 362 - 363, XP002213516
DUDAREWICZ, L. ET AL.: "Molecular methods for rapid detection of aneuploidy", J. APPL. GENET., vol. 46, no. 2, 2005, pages 207 - 215, XP055577114
TREFF, N. R. ET AL.: "Development and validation of an accurate quantitative real-time polymerase chain reaction-based assay for human blastocyst comprehensive chromosomal aneuploidy Screening", FERTIL. STERIL., vol. 97, no. 4, 2012, pages 819 - 824, XP028478554
HASHEMI, M. ET AL.: "A novel multiplex real-time PCR assay using TaqMan MGB probes for rapid detection of trisomy 21", MIDDLE EAST JOURNAL OF SCIENTIFIC RESEARCH, vol. 6, no. 4, 2010, pages 398 - 402, XP055675513, ISSN: 1990-9233
V. RUFFIO-CHABLE ET AL.: "Developmentally ''Aberrant'' plants in F1 hybrids of Brassica oleracea", ISHS ACTA HORTICULTURAE, vol. 539, 2000, pages 89
V. CHABLE ET AL.: "Aberrant'' plants in cauliflower: 2. Aneuploidy and global DNA methylation", EUPHYTICA, vol. 170, 2009, pages 275, XP019748278, DOI: 10.1007/s10681-009-9978-4
N. ROUX ET AL.: "Rapid detection of aneuploidy in Musa using flow cytometry", PLANT CELL REPORT, vol. 21, 2003, pages 483
I. PARKIN ET AL.: "Segmental structure of the Brassica napus genome based on comparative analysis with Arabidopsis thaliana", GENETICS, vol. 171, 2005, pages 765
X. CHENG ET AL.: "Development and genetic mapping of microsatellite markers from genome survey sequences in Brassica napus", THEORETICAL APPLIED GENETICS, vol. 118, 2009, pages 1121, XP019698439
SAIKI RK ET AL., SCIENCE, vol. 230, 1985, pages 1350 - 1354
"Plant PCR Experimental Protocol in Plant Cell Engineering", 1995
See also references of EP 3670657A4
Attorney, Agent or Firm:
KONNO, Akio et al. (JP)
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