Title:
METHOD FOR DETECTING VIRUS-NEUTRALIZING ANTIBODIES
Document Type and Number:
WIPO Patent Application WO/2022/215641
Kind Code:
A1
Abstract:
Provided is a method for evaluating virus-neutralizing antibodies using dried blood adhering to filter paper, the method including: a step for mixing a blood sample obtained by extracting the dried blood adhering to the filter paper and a pseudotype virus, which coats the coat protein of a target virus and in the genes of which a reporter gene is incorporated, to obtain a mixture; a step for infecting a susceptible cultured cell line with the psuedotype virus in the mixture; and a step for detecting the presence or measuring the amount of pseudotype-virus-neutralizing antibodies in the blood sample on the basis of the expression level of the reporter gene after infection.
Inventors:
MORINAGA YOSHITOMO (JP)
YAMAMOTO YOSHIHIRO (JP)
KAWASUJI HITOSHI (JP)
TANI HIDEKI (JP)
YAMAMOTO YOSHIHIRO (JP)
KAWASUJI HITOSHI (JP)
TANI HIDEKI (JP)
Application Number:
PCT/JP2022/016414
Publication Date:
October 13, 2022
Filing Date:
March 31, 2022
Export Citation:
Assignee:
NATIONAL UNIV CORPORATION UNIV OF TOYAMA (JP)
TOYAMA PREFECTURE (JP)
TOYAMA PREFECTURE (JP)
International Classes:
C12Q1/70; C12N5/071; C12N7/01; G01N33/53; G01N33/569; C07K16/10; C12Q1/66; C12Q1/6897
Foreign References:
| JP2003222627A | 2003-08-08 | |||
| CN111474257A | 2020-07-31 |
Other References:
TANI HIDEKI, KIMURA MIYUKI, TAN LONG, YOSHIDA YOSHIHIRO, OZAWA TATSUHIKO, KISHI HIROYUKI, FUKUSHI SHUETSU, SAIJO MASAYUKI, SANO KA: "Evaluation of SARS-CoV-2 neutralizing antibodies using a vesicular stomatitis virus possessing SARS-CoV-2 spike protein", VIROLOGY JOURNAL, vol. 18, no. 1, 1 December 2021 (2021-12-01), pages 16, XP055975032, DOI: 10.1186/s12985-021-01490-7
SCHMIDT FABIAN, WEISBLUM YISKA, MUECKSCH FRAUKE, HOFFMANN HANS-HEINRICH, MICHAILIDIS ELEFTHERIOS, LORENZI JULIO C. C., MENDOZA PIL: "Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses", BIORXIV, 9 June 2020 (2020-06-09), pages 1 - 44, XP055841655, Retrieved from the Internet [retrieved on 20210916], DOI: 10.1101/2020.06.08.140871
THEVIS MARIO, KNOOP ANDRE, SCHAEFER MAXIMILIAN S., DUFAUX BERTIN, SCHRADER YVONNE, THOMAS ANDREAS, GEYER HANS: "Can dried blood spots (DBS) contribute to conducting comprehensive SARS‐CoV‐2 antibody tests?", DRUG TESTING AND ANALYSIS, JOHN WILEY & SONS LTD., GB, vol. 12, no. 7, 1 July 2020 (2020-07-01), GB , pages 994 - 997, XP055975034, ISSN: 1942-7603, DOI: 10.1002/dta.2816
SCHMIDT FABIAN, WEISBLUM YISKA, MUECKSCH FRAUKE, HOFFMANN HANS-HEINRICH, MICHAILIDIS ELEFTHERIOS, LORENZI JULIO C. C., MENDOZA PIL: "Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses", BIORXIV, 9 June 2020 (2020-06-09), pages 1 - 44, XP055841655, Retrieved from the Internet
THEVIS MARIO, KNOOP ANDRE, SCHAEFER MAXIMILIAN S., DUFAUX BERTIN, SCHRADER YVONNE, THOMAS ANDREAS, GEYER HANS: "Can dried blood spots (DBS) contribute to conducting comprehensive SARS‐CoV‐2 antibody tests?", DRUG TESTING AND ANALYSIS, JOHN WILEY & SONS LTD., GB, vol. 12, no. 7, 1 July 2020 (2020-07-01), GB , pages 994 - 997, XP055975034, ISSN: 1942-7603, DOI: 10.1002/dta.2816
Attorney, Agent or Firm:
SAEGUSA & PARTNERS (JP)
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