Title:
METHOD FOR THE DETECTION OF FUSARIUM GRAMINEARUM
Document Type and Number:
WIPO Patent Application WO2006005770
Kind Code:
A3
Abstract:
A method for the detection of Fusarium graminearum (Gibberella zeae) comprising the steps: providing a sample containing a nucleic acid, contacting said sample with at least one forward primer and at least one reverse primer, wherein the at least one reverse primer hybridises within the ß-tubulin nucleic acid sequence of Fusarium graminearum (Gibberella zeae) and comprises the nucleic acid sequence 5'-R1TTTTCGTX1GX2AGT-3' (SEQ ID NO 1), wherein R1 comprises at least one nucleic acid residue of the ß-tubulin nucleic acid sequence located upstream of the hybridisation site of the nucleic acid sequence SEQ ID NO 1 and subsequent to the nucleic acid sequence SEQ ID NO 1, X1 is guanine, adenine or inosine, X2 is cytosine, adenine or inosine, and wherein the at least one forward primer hybridises upstream of the hybridisation site of a nucleic acid sequence complementary to the at least one reverse primer, subjecting the sample contacted with the at least one forward primer and the at least one reverse primer to a nucleic acid amplification technique, and optionally determing the presence of Fusarium graminearum (Gibberella zeae) in a sample by detecting a nucleic acid amplification product.
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Inventors:
MACH ROBERT (AT)
FARNLEITNER ANDREAS (AT)
REISCHER GEORG (AT)
FARNLEITNER ANDREAS (AT)
REISCHER GEORG (AT)
Application Number:
PCT/EP2005/053394
Publication Date:
May 18, 2006
Filing Date:
July 14, 2005
Export Citation:
Assignee:
UNIV WIEN TECH (AT)
MACH ROBERT (AT)
FARNLEITNER ANDREAS (AT)
REISCHER GEORG (AT)
MACH ROBERT (AT)
FARNLEITNER ANDREAS (AT)
REISCHER GEORG (AT)
International Classes:
C12Q1/68
Domestic Patent References:
| WO1999007884A1 | 1999-02-18 |
Other References:
EDWARDS S G ET AL: "QUANTIFICATION OF TRICHOTHECENE-PRODUCING FUSARIUM SPECIES IN HARVESTED GRAIN BY COMPETITIVE PCR TO DETERMINE EFFICACIES OF FUNGICIDES AGAINST FUSARIUM HEAD BLIGHT OF WINTER WHEAT", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 67, no. 4, April 2001 (2001-04-01), pages 1575 - 1580, XP001076826, ISSN: 0099-2240
MCCARTNEY H. A. ET AL.,: "molecular diagnostics for fungal pathogens", PEST MANAGEMENT SCIENCE, vol. 59, 2003, pages 129 - 142, XP002367388
FRAAIJE B.A.: "rapid detection and diagnosis of septoria tritici epidemics in wheat using a polymerase chain reaction/picogreen assay", J. APPLIED MICROBIOLOGY, vol. 86, 1999, pages 701 - 708, XP002367389
ATKINS S.D. & CLARK I.M.: "fungal molecular diagnostics: a mini review", J. APPL. GENET., vol. 45, no. 1, January 2004 (2004-01-01), pages 3 - 15, XP002367390
REISCHER G H ET AL: "Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM,, NL, vol. 59, no. 1, October 2004 (2004-10-01), pages 141 - 146, XP004533042, ISSN: 0167-7012
MCCARTNEY H. A. ET AL.,: "molecular diagnostics for fungal pathogens", PEST MANAGEMENT SCIENCE, vol. 59, 2003, pages 129 - 142, XP002367388
FRAAIJE B.A.: "rapid detection and diagnosis of septoria tritici epidemics in wheat using a polymerase chain reaction/picogreen assay", J. APPLIED MICROBIOLOGY, vol. 86, 1999, pages 701 - 708, XP002367389
ATKINS S.D. & CLARK I.M.: "fungal molecular diagnostics: a mini review", J. APPL. GENET., vol. 45, no. 1, January 2004 (2004-01-01), pages 3 - 15, XP002367390
REISCHER G H ET AL: "Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM,, NL, vol. 59, no. 1, October 2004 (2004-10-01), pages 141 - 146, XP004533042, ISSN: 0167-7012
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