Title:
METHOD OF DETECTION, SEPARATION AND IDENTIFICATION FOR EXPRESSED TRACE PROTEIN/PEPTIDE
Document Type and Number:
WIPO Patent Application WO2005056146
Kind Code:
A3
Abstract:
A method of detection, separation and identification for expressed trace protein/peptide; and a system therefor. There is provided a method of detecting, separating and identifying a minute amount of expressed protein and/or peptide, characterized in that a fluorescent derivative of protein and/or peptide contained in a test subject sample having been labeled with a fluorescence reagent is applied to HPLC; a fluorescent fraction is collected and subjected to enzymatic hydrolysis; mass-spectrometry of the resultant fluorescence-labeled fragments and non-fluorescence-labeled fragments is carried out; and the thus obtained ion molecular weight information on each of the fragments is collated with an available protein and/or peptide fragment database to thereby accomplish a structural analysis. Further, there is provided an identification system therefor.
Inventors:
IMAI KAZUHIRO (JP)
Application Number:
PCT/JP2004/018592
Publication Date:
August 18, 2005
Filing Date:
December 13, 2004
Export Citation:
Assignee:
IMAI KAZUHIRO (JP)
International Classes:
G01N21/64; G01N21/77; G01N21/78; G01N27/62; G01N30/88; G01N33/483; G01N33/533; G01N33/68; G01N27/447; G01N30/02; G01N30/72; B01D; (IPC1-7): G01N27/62; G01N21/77; G01N21/78; G01N30/88; G01N33/483
Domestic Patent References:
| WO2003002226A1 | 2003-01-09 | |||
| WO2003029425A2 | 2003-04-10 | |||
| WO2003054518A1 | 2003-07-03 |
Other References:
See also references of EP 1705482A4
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