Title:
METHOD OF DETERMINATION OF DIAGNOSIS AND PROGNOSIS IN PATIENTS WITH B-CELL CHRONIC LYMPHOCYTIC LEUKEMIA AND OLIGONUCLEOTIDES FOR USE IN THIS METHOD
Document Type and Number:
WIPO Patent Application WO/2011/015162
Kind Code:
A3
Abstract:
The invention provides a method of determination of diagnosis and prognosis of B-cell chronic lymphocytic leukemia from a biological sample collected from the body of a patient, wherein the status of Wnt/PCP signaling pathway is determined. Within the framework of the present invention the relation of CLL and molecular signaling pathway Wnt/PCP the components of which are markedly up-regulated in B-lymphocytes of the patients suffering from CLL was identified. The status of the Wnt/PCP signaling pathway can be determined, e.g., by the determination of the expression of components of said signaling pathway or by the determination of migration of CLL cells in the gradient of a chemokine in the presence of the ligand of said signaling pathway. The invention also relates to suitable oligonucleotides for use in the method of determination of expression of the signaling pathway components.
Inventors:
BRYJA VITEZSLAV (CZ)
KREJCI PAVEL (CZ)
KAUCKA MARKETA (CZ)
POSPISILOVA SARKA (CZ)
MAYER JIRI (CZ)
KOZUBIK ALOIS (CZ)
PLEVOVA KARLA (CZ)
KOTASKOVA JANA (CZ)
KREJCI PAVEL (CZ)
KAUCKA MARKETA (CZ)
POSPISILOVA SARKA (CZ)
MAYER JIRI (CZ)
KOZUBIK ALOIS (CZ)
PLEVOVA KARLA (CZ)
KOTASKOVA JANA (CZ)
Application Number:
PCT/CZ2010/000090
Publication Date:
June 03, 2011
Filing Date:
August 03, 2010
Export Citation:
Assignee:
UNIV MASARYKOVA (CZ)
BRYJA VITEZSLAV (CZ)
KREJCI PAVEL (CZ)
KAUCKA MARKETA (CZ)
POSPISILOVA SARKA (CZ)
MAYER JIRI (CZ)
KOZUBIK ALOIS (CZ)
PLEVOVA KARLA (CZ)
KOTASKOVA JANA (CZ)
BRYJA VITEZSLAV (CZ)
KREJCI PAVEL (CZ)
KAUCKA MARKETA (CZ)
POSPISILOVA SARKA (CZ)
MAYER JIRI (CZ)
KOZUBIK ALOIS (CZ)
PLEVOVA KARLA (CZ)
KOTASKOVA JANA (CZ)
International Classes:
C12Q1/686; C12Q1/6876; C12Q1/6883; C12Q1/6888
Other References:
KATOH MASARU: "WNT/PCP signaling pathway and human cancer (review)", ONCOLOGY REPORTS, vol. 14, no. 6, 1 December 2005 (2005-12-01), SPANDIDOS PUBLICATIONS, GR, pages 1583 - 1588, XP009104214, ISSN: 1021-335X
MAHADEVAN DARUKA ET AL: "Gene Expression and Serum Cytokine Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3 and CXCL9/CXCR3 as Regulators of Cell Proliferation, Survival and Migration.", HUMAN GENOMICS AND PROTEOMICS, vol. 2009, 453634, 24 June 2009 (2009-06-24), pages 1 - 12, XP002626571, ISSN: 1757-4242
WANG Z ET AL: "Amino acid limitation induces down-regulation of WNT5a at transcriptional level", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 378, no. 4, 23 January 2009 (2009-01-23), ACADEMIC PRESS INC. ORLANDO, FL, US, pages 789 - 794, XP025841497, ISSN: 0006-291X, [retrieved on 20081206], DOI: 10.1016/J.BBRC.2008.11.124
LU DESHENG ET AL: "Activation of the Wnt signaling pathway in chronic lymphocytic leukemia", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES (PNAS), vol. 101, no. 9, 2 March 2004 (2004-03-02), pages 3118 - 3123, XP002466299, ISSN: 0027-8424, DOI: 10.1073/PNAS.0308648100
MAHADEVAN DARUKA ET AL: "Gene Expression and Serum Cytokine Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3 and CXCL9/CXCR3 as Regulators of Cell Proliferation, Survival and Migration.", HUMAN GENOMICS AND PROTEOMICS, vol. 2009, 453634, 24 June 2009 (2009-06-24), pages 1 - 12, XP002626571, ISSN: 1757-4242
WANG Z ET AL: "Amino acid limitation induces down-regulation of WNT5a at transcriptional level", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 378, no. 4, 23 January 2009 (2009-01-23), ACADEMIC PRESS INC. ORLANDO, FL, US, pages 789 - 794, XP025841497, ISSN: 0006-291X, [retrieved on 20081206], DOI: 10.1016/J.BBRC.2008.11.124
LU DESHENG ET AL: "Activation of the Wnt signaling pathway in chronic lymphocytic leukemia", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES (PNAS), vol. 101, no. 9, 2 March 2004 (2004-03-02), pages 3118 - 3123, XP002466299, ISSN: 0027-8424, DOI: 10.1073/PNAS.0308648100
Attorney, Agent or Firm:
HARTVICHOVA, Katerina (Na Belidle 3, Praha 5, CZ)
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