METHOD FOR DETERMINATION OF INHIBITORY ACTIVITY ON DNA METHYLATION

WIPO Patent Application WO/2008/029668

A1

Disclosed is a method for detecting an inhibitor substance which inhibits the methylation of DNA rapidly and in a simple manner without the need of using a sample in a large quantity and which can be used for the screening for a DNA methylation inhibitor. The method comprises the steps of: mixing a test DNA having a methylation site therein and having a fluorescent label attached thereto, a substance to be tested and a DNA-methylating enzyme; reacting the test DNA with a methylation-sensitive active substance capable of cleaving an unmethylated methylation site selectively; measuring a fluorescence intensity of the test DNA after the reaction with the methylation-sensitive restriction enzyme; and determining whether or not the test DNA is cleaved with the methylation-sensitive active substance based on the fluorescence intensity, wherein it is determined whether or not the substance to be tested is a inhibitor substance capable of inhibiting the methylation of the test DNA depending on whether or not the test DNA is cleaved.

YAMADA, Yoshifumi (43-2 Hatagaya 2-chom, Shibuya-ku Tokyo 72, 1510072, JP)
山田純史 (〒72 東京都渋谷区幡ヶ谷２丁目４３番２号オリンパス株式会社内 Tokyo, 1510072, JP)

JP2007/066624

March 13, 2008

August 28, 2007

Click for automatic bibliography generation  

OLYMPUS CORPORATION (43-2, Hatagaya 2-chome Shibuya-ku Tokyo, 72, 1510072, JP)
オリンパス株式会社 (〒72 東京都渋谷区幡ヶ谷２丁目４３番２号 Tokyo, 1510072, JP)
YAMADA, Yoshifumi (43-2 Hatagaya 2-chom, Shibuya-ku Tokyo 72, 1510072, JP)

C12Q1/68

AKASHI, Masaki et al. (5F YH Bldg, 6-8 Shinkawa 2-chom, Chuo-ku Tokyo 33, 1040033, JP)

View/Download PDF      PDF Help

Previous Patent: METHOD OF DETECTING BINDING SITE TO DNA-BINDING PROTEIN IN DNA

Next Patent: DISPLAY ELEMENT