Title:
METHOD FOR DETERMINATION WHETHER OR NOT CELL POPULATION IS SUITABLE FOR FOREIGN GENE TRANSFER FOR THE PURPOSE OF PREPARATION OF ADIPOCYTES FOR GENE THERAPY APPLICATION
Document Type and Number:
WIPO Patent Application WO/2011/090091
Kind Code:
A1
Abstract:
Disclosed is a method for determining whether or not adipocytes are a cell population suitable for the gene transfer of a foreign gene in the preparation of the adipocytes for use in a gene therapy comprising introducing the foreign gene using a viral vector. In the method, the suitability is determined on the basis of the level of the expression of CD54 in the adipocytes, and it is determined that the adipocytes having a lower expression level of CD54 are suitable. An example of the cell population of adipocytes suitable for the foreign gene transfer is a cell population in which the positive rate for the expression of CD54 is less than 3% and/or the average fluorescence intensity ratio for CD54 is less than 2 as measured by a flow cytometry using a fluorescent antibody.
Inventors:
ASO MASAYUKI (JP)
BUJO HIDEAKI (JP)
SAITO YASUSHI (JP)
KURODA MASAYUKI (JP)
BUJO HIDEAKI (JP)
SAITO YASUSHI (JP)
KURODA MASAYUKI (JP)
Application Number:
PCT/JP2011/050919
Publication Date:
July 28, 2011
Filing Date:
January 20, 2011
Export Citation:
Assignee:
CELLGENTECH INC (JP)
UNIV CHIBA NAT UNIV CORP (JP)
ASO MASAYUKI (JP)
BUJO HIDEAKI (JP)
SAITO YASUSHI (JP)
KURODA MASAYUKI (JP)
UNIV CHIBA NAT UNIV CORP (JP)
ASO MASAYUKI (JP)
BUJO HIDEAKI (JP)
SAITO YASUSHI (JP)
KURODA MASAYUKI (JP)
International Classes:
C12N5/10; C12Q1/02; G01N33/483; G01N33/53; G01N33/533; C12N15/09
Domestic Patent References:
| WO2008108344A1 | 2008-09-12 | |||
| WO2006134871A1 | 2006-12-21 | |||
| WO1997049827A2 | 1997-12-31 | |||
| WO2003106663A1 | 2003-12-24 |
Foreign References:
| JP2000083656A | 2000-03-28 |
Other References:
MASAYUKI ASO: "Idenshi Donyu Saibo Iyakuhin ni yoru Kisho Shikkan Chiryo eno Koken", GEKKAN PHARM STAGE, vol. 8, no. 6, 2008, pages 60 - 61
MIYAZAKI, T. ET AL.: "isolation of two numan fibroblastic cell populations with multiple but distinct potential of mesenchymal differentiation by ceiling culture of mature fat cells from subcutaneous adipose tissue", DIFFERENTIATION; RESEARCH IN BIOLOGICAL DIVERSITY, vol. 73, no. 2-3, 2005, pages 69 - 78
MIYAZAKI, T. ET AL.: "isolation of two numan fibroblastic cell populations with multiple but distinct potential of mesenchymal differentiation by ceiling culture of mature fat cells from subcutaneous adipose tissue", DIFFERENTIATION; RESEARCH IN BIOLOGICAL DIVERSITY, vol. 73, no. 2-3, 2005, pages 69 - 78
Attorney, Agent or Firm:
TSUJITA Takashi et al. (JP)
Yukifumi Tsujita (JP)
Yukifumi Tsujita (JP)
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Claims: