METHOD FOR DETERMINING THE ACTIVITY OF A PROTEOLYTIC ENZYME (VARIANTS), DEVICE FOR THE IMPLEMENTATION OF SAME AND DIAGNOSTIC METHOD

WIPO Patent Application WO/2013/015717

A8

The invention relates to the field of biotechnology. The method for determining the spatial and temporal distribution of the activity of a proteolytic enzyme in an in vitro heterogeneous system, such as blood or blood plasma, involves the introduction of a luminescent, fluorogenic or chromogenic substrate into a sample with the subsequent release of a detectable tag as the proteolytic enzyme cleaves the substrate, and the recording of the optical characteristics of the sample, which makes it possible to assess the spatial and temporal distribution of the activity of the enzyme. The device for the implementation of the above method comprises an in vitro system, a means for illuminating the sample, a recording means and a control means. A method for diagnosing homeostatic imbalances according to a change in the spatial and temporal distribution of the activity of a proteolytic enzyme in a blood sample is also proposed.

ATAULLAKHANOV FAZOIL INOYATOVICH (RU)
DASHKEVICH NATALJYA MIKHAJLOVNA (RU)
OVANESOV MIKHAIL VLADIMIROVICH (RU)
SARBASH VASILII IVANOVICH (RU)
PANTELEEV MIKHAIL ALEKSANDROVICH (RU)
KARAMZIN SERGEY SERGEEVICH (RU)
KONDRATOVICH ANDREY YURJEVICH (RU)

PCT/RU2012/000570

February 20, 2014

July 16, 2012

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OBSCHESTVO S OGRANICHENNOY OTVETSTVENNOSTYU GEMATOLOGICHESKAYA KORPORATSIYA (RU)
ATAULLAKHANOV FAZOIL INOYATOVICH (RU)
DASHKEVICH NATALJYA MIKHAJLOVNA (RU)
OVANESOV MIKHAIL VLADIMIROVICH (RU)
SARBASH VASILII IVANOVICH (RU)
PANTELEEV MIKHAIL ALEKSANDROVICH (RU)
KARAMZIN SERGEY SERGEEVICH (RU)
KONDRATOVICH ANDREY YURJEVICH (RU)

C12Q1/56; G01N21/64; G01N21/76; G01N33/48

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