Title:
METHOD FOR DETERMINING CHROMATIN STRUCTURE
Document Type and Number:
WIPO Patent Application WO2003004702
Kind Code:
A3
Abstract:
A method of determining chromatin structure is described. The method comprises the steps of (i) fragmenting a nucleotide sequence at multiple HSs; and (ii) analysing fragments formed in step (i) from a plurality of sequences. In a preferred aspect, the present invention provides a method of determining chromatin structure in a nucleic acid sample comprising the steps of (i) treating the sample to fragment the nucleic acid therein at multiple HSs; and (ii) analysing fragments formed in step (i) from a plurality of genes.
Inventors:
WEINZIERL ROBERT OTTO JOHANNES (GB)
Application Number:
PCT/GB2002/003080
Publication Date:
October 16, 2003
Filing Date:
July 04, 2002
Export Citation:
Assignee:
IMP COLLEGE INNOVATIONS LTD (GB)
WEINZIERL ROBERT OTTO JOHANNES (GB)
WEINZIERL ROBERT OTTO JOHANNES (GB)
International Classes:
A61P43/00; C12Q1/68; C12Q1/683; (IPC1-7): C12Q1/68; A61P43/00
Other References:
HATADA I ET EL: "A genomic scanning method for higher organisms using restriction sites and landmarks", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 88, November 1991 (1991-11-01), pages 9523 - 9527, XP002953607, ISSN: 0027-8424
PFEIFER G P ET AL: "Chromatin structure analysis by ligation-mediated and terminal transferase-mediated polymerase chain reaction.", METHODS IN ENZYMOLOGY, vol. 304, 1999, 1999 Academic Press Inc.; Academic Press Ltd. 525 B Street, Suite 1900, San Diego, CA, 92101-4495, USA; 24-28 Oval Road, London, NW1 7DX, UK, pages 548 - 571, XP001147579, ISSN: 0-12-1822-5-2
GROSS D S ET AL: "NUCLEASE HYPERSENSITIVE SITES IN CHROMATIN", ANNUAL REVIEW OF BIOCHEMISTRY, PALTO ALTO, CA, US, vol. 57, 1988, pages 159 - 197, XP000879087, ISSN: 0066-4154
HUBER MATTHIAS C ET AL: "Dynamic changes in the chromatin of the chicken lysozyme gene domain during differentiation of multipotent progenitors to macrophages.", DNA AND CELL BIOLOGY, vol. 14, no. 5, 1995, pages 397 - 402, XP001152643, ISSN: 1044-5498
SMITH C L ET AL: "Strategies for mapping and cloning macroregions of mammalian genomes.", METHODS IN ENZYMOLOGY. UNITED STATES 1987, vol. 151, 1987, pages 461 - 489, XP001147570, ISSN: 0076-6879
PFEIFER G P ET AL: "Chromatin structure analysis by ligation-mediated and terminal transferase-mediated polymerase chain reaction.", METHODS IN ENZYMOLOGY, vol. 304, 1999, 1999 Academic Press Inc.; Academic Press Ltd. 525 B Street, Suite 1900, San Diego, CA, 92101-4495, USA; 24-28 Oval Road, London, NW1 7DX, UK, pages 548 - 571, XP001147579, ISSN: 0-12-1822-5-2
GROSS D S ET AL: "NUCLEASE HYPERSENSITIVE SITES IN CHROMATIN", ANNUAL REVIEW OF BIOCHEMISTRY, PALTO ALTO, CA, US, vol. 57, 1988, pages 159 - 197, XP000879087, ISSN: 0066-4154
HUBER MATTHIAS C ET AL: "Dynamic changes in the chromatin of the chicken lysozyme gene domain during differentiation of multipotent progenitors to macrophages.", DNA AND CELL BIOLOGY, vol. 14, no. 5, 1995, pages 397 - 402, XP001152643, ISSN: 1044-5498
SMITH C L ET AL: "Strategies for mapping and cloning macroregions of mammalian genomes.", METHODS IN ENZYMOLOGY. UNITED STATES 1987, vol. 151, 1987, pages 461 - 489, XP001147570, ISSN: 0076-6879
Download PDF: