Title:
METHOD FOR DETERMINING ENZYMATIC ACTIVITY OF GLYCOSYL TRANSFERASE
Document Type and Number:
WIPO Patent Application WO2002092845
Kind Code:
A3
Abstract:
The invention relates to a method for determining the enzymatic activity of glycosyl transferase in a sample, wherein the sample is reacted with a glycosyl donor and a glycosyl receptor immobilized on a carrier, especially a cellulose membrane, wherein a transferase product is determined in the presence of a enzymatic glycosyl transferase activity and the carrier is a cellulose membrane.
Inventors:
HUMMEL GERD (DE)
JOBRON LAURENCE (DE)
SUJINO KEIKO (CA)
PALCIC MONICA M (CA)
JOBRON LAURENCE (DE)
SUJINO KEIKO (CA)
PALCIC MONICA M (CA)
Application Number:
PCT/EP2002/005101
Publication Date:
March 11, 2004
Filing Date:
May 08, 2002
Export Citation:
Assignee:
JERINI AG (DE)
HUMMEL GERD (DE)
JOBRON LAURENCE (DE)
SUJINO KEIKO (CA)
PALCIC MONICA M (CA)
HUMMEL GERD (DE)
JOBRON LAURENCE (DE)
SUJINO KEIKO (CA)
PALCIC MONICA M (CA)
International Classes:
C12Q1/48; (IPC1-7): C12Q1/48; C12N9/10
Domestic Patent References:
| WO1994010340A1 | 1994-05-11 |
Foreign References:
| US5173407A | 1992-12-22 | |||
| EP0387875A2 | 1990-09-19 |
Other References:
SADLER J E ET AL: "PURIFICATION OF MAMMALIAN GLYCOSYLTRANSFERASES", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 83, 1982, pages 458 - 514, XP000749292, ISSN: 0076-6879
WENSCHUH HOLGER ET AL: "Coherent membrane supports for parallel microsynthesis and screening of bioactive peptides.", BIOPOLYMERS, vol. 55, no. 3, 2000, pages 188 - 206, XP002255029, ISSN: 0006-3525
BAXTER A ET AL: "A RAPID SENSITIVE DISC ASSAY FOR THE DETERMINATION OF GLYCO PROTEIN GLYCOSYL TRANSFERASES", ANALYTICAL BIOCHEMISTRY, vol. 98, no. 1, 1979, pages 95 - 101, XP009011760, ISSN: 0003-2697
REINEKE U ET AL: "Applications of peptide arrays prepared by the SPOT-technology", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 12, no. 1, February 2001 (2001-02-01), pages 59 - 64, XP002234261, ISSN: 0958-1669
GU, XINBIN ET AL: "Direct Assay of Glycosphingolipid Glycosyltransferase Activities on Thin-Layer Chromatogram", ANALTICAL BIOCHEMISTRY, vol. 185, 1990, pages 151 - 155, XP009017591
WENSCHUH HOLGER ET AL: "Coherent membrane supports for parallel microsynthesis and screening of bioactive peptides.", BIOPOLYMERS, vol. 55, no. 3, 2000, pages 188 - 206, XP002255029, ISSN: 0006-3525
BAXTER A ET AL: "A RAPID SENSITIVE DISC ASSAY FOR THE DETERMINATION OF GLYCO PROTEIN GLYCOSYL TRANSFERASES", ANALYTICAL BIOCHEMISTRY, vol. 98, no. 1, 1979, pages 95 - 101, XP009011760, ISSN: 0003-2697
REINEKE U ET AL: "Applications of peptide arrays prepared by the SPOT-technology", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 12, no. 1, February 2001 (2001-02-01), pages 59 - 64, XP002234261, ISSN: 0958-1669
GU, XINBIN ET AL: "Direct Assay of Glycosphingolipid Glycosyltransferase Activities on Thin-Layer Chromatogram", ANALTICAL BIOCHEMISTRY, vol. 185, 1990, pages 151 - 155, XP009017591
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