Title:
METHOD FOR DETERMINING AN INTEGRAL SUN PROTECTION FACTOR ENCOMPASSING UVA AND UVB RADIATION
Document Type and Number:
WIPO Patent Application WO2005103659
Kind Code:
A3
Abstract:
The invention relates to a method for determining an integral sun protection factor (ò SPF or iSPF) which encompasses both UVA and UVB radiation and can be used for classifying cosmetic and dermatological sunscreens. The method comprises the steps of applying a defined amount of the sunscreen onto a defined area of a skin substrate impregnated with a spin trap, exposing the skin substrate to a defined amount of UVA- and UVB rays by a sun simulator, subjecting the skin substrate exposed to radiation to an electron spin resonance (ESR) measurement, recording the number of free radicals trapped as a real number x 1012 free radicals / mg of skin sample, and determining the integral sun protection factor ò SPF according to the equations (1) and (2) and (8).
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Inventors:
FERRERO LOUIS (FR)
ZASTROW LEONHARD (MC)
GOLZ-BERNER KARIN (MC)
GROTH NORBERT (DE)
HERRLING THOMAS (DE)
ZASTROW LEONHARD (MC)
GOLZ-BERNER KARIN (MC)
GROTH NORBERT (DE)
HERRLING THOMAS (DE)
Application Number:
PCT/EP2005/004148
Publication Date:
March 09, 2006
Filing Date:
April 14, 2005
Export Citation:
Assignee:
COTY BV (NL)
FERRERO LOUIS (FR)
ZASTROW LEONHARD (MC)
GOLZ-BERNER KARIN (MC)
GROTH NORBERT (DE)
HERRLING THOMAS (DE)
FERRERO LOUIS (FR)
ZASTROW LEONHARD (MC)
GOLZ-BERNER KARIN (MC)
GROTH NORBERT (DE)
HERRLING THOMAS (DE)
International Classes:
G01R33/60; (IPC1-7): G01R33/60
Other References:
T. HERRLING ET AL: "UV-induced free radicals in the skin detected by ESR spectroscopy and imaging using nitroxides", FREE RADICAL BIOLOGY AND MEDICINE, vol. 35, 2003, pages 59 - 67, XP002345868
HAYWOOD RACHEL ET AL: "Sunscreens inadequately protect against ultraviolet-A-induced free radicals in skin: Implications for skin aging and melanom?", JOURNAL OF INVESTIGATIVE DERMATOLOGY, NEW YORK, NY, US, vol. 121, no. 4, October 2003 (2003-10-01), pages 862 - 868, XP002271671, ISSN: 0022-202X
HITOSHI MASAKI ET AL.: "detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 206, no. 2, 1995, pages 474 - 479, XP002345869
HITOSHI MASAKI ET AL.: "increased generation of hydrogen peroxide possibly from mitochondrial respiratory chain after UVB irradiation of murine fibroblasts", JOURNAL OF DERMATOLOGICAL SCIENCE, vol. 14, 1977, pages 207 - 216, XP002345870
FRANCOISE BERNERD ET AL.: "The sun protection factor (SPF) inadequately defines broad spectrum photoprotection: demonstration using skin reconstructed in vitro exposed to UVA, UVB or UV-solar simulated radiation", EUROPEAN JOURNAL OF DERMATOLOGY, vol. 13, 2003, pages 242 - 249, XP002345871
HAYWOOD RACHEL ET AL: "Sunscreens inadequately protect against ultraviolet-A-induced free radicals in skin: Implications for skin aging and melanom?", JOURNAL OF INVESTIGATIVE DERMATOLOGY, NEW YORK, NY, US, vol. 121, no. 4, October 2003 (2003-10-01), pages 862 - 868, XP002271671, ISSN: 0022-202X
HITOSHI MASAKI ET AL.: "detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 206, no. 2, 1995, pages 474 - 479, XP002345869
HITOSHI MASAKI ET AL.: "increased generation of hydrogen peroxide possibly from mitochondrial respiratory chain after UVB irradiation of murine fibroblasts", JOURNAL OF DERMATOLOGICAL SCIENCE, vol. 14, 1977, pages 207 - 216, XP002345870
FRANCOISE BERNERD ET AL.: "The sun protection factor (SPF) inadequately defines broad spectrum photoprotection: demonstration using skin reconstructed in vitro exposed to UVA, UVB or UV-solar simulated radiation", EUROPEAN JOURNAL OF DERMATOLOGY, vol. 13, 2003, pages 242 - 249, XP002345871
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