Title:
METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES
Document Type and Number:
WIPO Patent Application WO2003020895
Kind Code:
A3
Abstract:
The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
Inventors:
WILLIAMS PETER
TAYLOR THOMAS J
WILLIAMS DANIEL J B
GOULD IAN
HAYES MARK A
TAYLOR THOMAS J
WILLIAMS DANIEL J B
GOULD IAN
HAYES MARK A
Application Number:
PCT/US2002/027605
Publication Date:
August 21, 2003
Filing Date:
August 28, 2002
Export Citation:
Assignee:
UNIV ARIZONA (US)
International Classes:
C12Q1/68; C12N15/09; (IPC1-7): C12Q1/68; C12P19/34; C07H19/00; C07H21/00
Domestic Patent References:
| WO1989009283A1 | 1989-10-05 | |||
| WO1991006678A1 | 1991-05-16 |
Foreign References:
| EP0223618A2 | 1987-05-27 | |||
| US5436149A | 1995-07-25 |
Other References:
See also references of EP 1427851A4
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