Title:
METHOD FOR DETERMINING PRESENCE OR ABSENCE OF CANCER CELL DERIVED FROM HEPATOCELLULAR CARCINOMA, AND DETERMINATION MARKER AND KIT
Document Type and Number:
WIPO Patent Application WO/2013/129397
Kind Code:
A1
Abstract:
The present invention relates to a method for analyzing the state of methylation of a CpG site occurring in a promoter region for at least one gene selected from ACTG1, EPHA4 and TSC22D1 that are contained in DNA extracted from a biological sample and determining the presence or absence of a cancer cell derived from hepatocellular carcinoma on the basis of the results of the analysis. The present invention also relates to a determination maker and a kit, both of which can be used in the method.
Inventors:
SAKAI AYAKO (JP)
NAGAE GENTA (JP)
MIDORIKAWA YUTAKA (JP)
KAJITA MASAHIRO (JP)
ABURATANI HIROYUKI (JP)
NAGAE GENTA (JP)
MIDORIKAWA YUTAKA (JP)
KAJITA MASAHIRO (JP)
ABURATANI HIROYUKI (JP)
Application Number:
PCT/JP2013/054961
Publication Date:
September 06, 2013
Filing Date:
February 26, 2013
Export Citation:
Assignee:
SYSMEX CORP (JP)
UNIV TOKYO (JP)
SAKAI AYAKO (JP)
NAGAE GENTA (JP)
MIDORIKAWA YUTAKA (JP)
KAJITA MASAHIRO (JP)
ABURATANI HIROYUKI (JP)
UNIV TOKYO (JP)
SAKAI AYAKO (JP)
NAGAE GENTA (JP)
MIDORIKAWA YUTAKA (JP)
KAJITA MASAHIRO (JP)
ABURATANI HIROYUKI (JP)
International Classes:
C12N15/09; C12Q1/68; G01N27/62
Foreign References:
| JP2011177139A | 2011-09-15 |
Other References:
DENG, Y.B. ET AL.: "Identification of genes preferentially methylated in hepatitis C virus-related hepatocellular carcinoma.", CANCER SCI., vol. 101, no. 6, 2010, pages 1501 - 1510, XP055163819
TAO, R. ET AL.: "Methylation profile of single hepatocytes derived from hepatitis B virus- related hepatocellular carcinoma.", PLOS ONE, vol. 6, no. 5, 2011, pages E19862, XP055163821
SHIN, S.H. ET AL.: "Identification of novel methylation markers in hepatocellular carcinoma using a methylation array.", J. KOREAN MED. SCI., vol. 25, no. 8, 2010, pages 1152 - 1159, XP055163822
HERNANDEZ-VARGAS, H. ET AL.: "Hepatocellular carcinoma displays distinct DNA methylation signatures with potential as clinical predictors.", PLOS ONE, vol. 5, no. 3, 2010, pages E9749, XP055163824
BIBIKOVA, M. ET AL.: "High-throughput DNA methylation profiling using universal bead arrays.", GENOME RES., vol. 16, no. 3, 2006, pages 383 - 393, XP002445638
See also references of EP 2821487A4
YING-BING DENG ET AL., CANCER SCI, vol. 101, no. 6, June 2010 (2010-06-01), pages 1501 - 1510
RAN TAO ET AL., PROS ONE, vol. 6, no. 5, May 2011 (2011-05-01), pages E19862
TAO, R. ET AL.: "Methylation profile of single hepatocytes derived from hepatitis B virus- related hepatocellular carcinoma.", PLOS ONE, vol. 6, no. 5, 2011, pages E19862, XP055163821
SHIN, S.H. ET AL.: "Identification of novel methylation markers in hepatocellular carcinoma using a methylation array.", J. KOREAN MED. SCI., vol. 25, no. 8, 2010, pages 1152 - 1159, XP055163822
HERNANDEZ-VARGAS, H. ET AL.: "Hepatocellular carcinoma displays distinct DNA methylation signatures with potential as clinical predictors.", PLOS ONE, vol. 5, no. 3, 2010, pages E9749, XP055163824
BIBIKOVA, M. ET AL.: "High-throughput DNA methylation profiling using universal bead arrays.", GENOME RES., vol. 16, no. 3, 2006, pages 383 - 393, XP002445638
See also references of EP 2821487A4
YING-BING DENG ET AL., CANCER SCI, vol. 101, no. 6, June 2010 (2010-06-01), pages 1501 - 1510
RAN TAO ET AL., PROS ONE, vol. 6, no. 5, May 2011 (2011-05-01), pages E19862
Attorney, Agent or Firm:
NOGAWA, Shintaro et al. (JP)
Shintaro Nogawa (JP)
Shintaro Nogawa (JP)
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Claims: