METHOD AND DEVICE FOR CORRECTING LEVEL OF EXPRESSION OF SMALL RNA

Title:

METHOD AND DEVICE FOR CORRECTING LEVEL OF EXPRESSION OF SMALL RNA

Document Type and Number:

WIPO Patent Application WO/2016/084848

Kind Code:

A1

Abstract:

Disclosed is a means capable of accurately correcting a measurement value of the level of expression of target small RNA among a plurality of specimens using a target substance in order to comparatively analyze the expression level. In the method for correcting the level of expression of small RNA in the present invention, a nucleic acid that is 200 bases or more in length is used as the target substance. A fixed quantity of the target substance is added to a fixed quantity of each specimen, nucleic acid is extracted from the specimens, the amounts of the extracted target small RNA and target substance are measured, and the level of expression of the target small RNA is corrected using the measured value of the extracted amount of target substance. According to the present invention, the level of expression of small RNA among specimens can be corrected with greater accuracy than conventional methods.

Inventors:

KONDOU SATOSHI (JP)
KOZONO SATOKO (JP)

Application Number:

PCT/JP2015/083079

Publication Date:

June 02, 2016

Filing Date:

November 25, 2015

Export Citation:

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Assignee:

TORAY INDUSTRIES (JP)

International Classes:

C12N15/09; G16B25/10; C12M1/00; C12Q1/68

Domestic Patent References:

WO2011145614A1

2011-11-24

Foreign References:

JP2007075095A	2007-03-29
JP2007097429A	2007-04-19
JP2014007995A	2014-01-20
JP2010119331A	2010-06-03
JP2010519893A	2010-06-10

Other References: