Title:
METHOD AND DEVICE FOR DISTINGUISHING MULTIPLE NUCLEIC ACID SPECIMENS IMMOBILIZED ON SUBSTRATE
Document Type and Number:
WIPO Patent Application WO/2011/108344
Kind Code:
A1
Abstract:
Provided is a method whereby multiple kinds of nucleic acid specimens, which are immobilized on a substrate, can be distinguished without a need for separately adding index sequences for distinguishing the nucleic acid specimens or immobilizing the nucleic acid specimens at sites on the substrate that have been intentionally determined for distinguishing the nucleic acid specimens. This method comprises: preparing multiple kinds of nucleic acid specimens to be distinguished which are immobilized at random on the surface of the substrate and carry signal probes [P(L)]; detecting signals derived from the signal probes [P(L)]; acquiring, based on the aforesaid signals, the immobilization site data on the substrate of the individual nucleic acid specimens of multiple kinds having been immobilized as described above; and distinguishing the multiple kinds of nucleic acid specimens one another based on the site data.
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Inventors:
KAWABE TAKASHI (JP)
WADA YUKIHISA (JP)
MORIYA NAOJI (JP)
WADA YUKIHISA (JP)
MORIYA NAOJI (JP)
Application Number:
PCT/JP2011/052881
Publication Date:
September 09, 2011
Filing Date:
February 10, 2011
Export Citation:
Assignee:
SHIMADZU CORP (JP)
KAWABE TAKASHI (JP)
WADA YUKIHISA (JP)
MORIYA NAOJI (JP)
KAWABE TAKASHI (JP)
WADA YUKIHISA (JP)
MORIYA NAOJI (JP)
International Classes:
C12Q1/68; C12M1/00; C12N15/09
Other References:
DAVID W CRAIG ET AL.: "Identification of genetic variants using bar-coded multiplexed sequencing", NATURE METHODS, vol. 5, no. 10, October 2008 (2008-10-01), pages 887 - 893, XP002662394, DOI: doi:10.1038/NMETH.1251
MIO TONOUCHI: "Jisedai Sequencer de Gan Genome o Kaimei susu: Illumina Genome Analyzer", BIO CLINICA, vol. 23, no. 9, 2008, pages 817 - 821
MASAFUMI YODA: "Jisedai Genome Kaiseki Gijutsu - Yotsudomoe no Tatakai no Yukue", BIO TECHNOLOGY JOURNAL, 2007, pages 392 - 393
MIO TOMOUCHI: "Genome Kaiseki no Kakumeiteki Tool: Illumina Genome Analyzer", BIO TECHNOLOGY JOURNAL, vol. 11, 2007, pages 742 - 743
MIO TONOUCHI: "Jisedai Sequencer de Gan Genome o Kaimei susu: Illumina Genome Analyzer", BIO CLINICA, vol. 23, no. 9, 2008, pages 817 - 821
MASAFUMI YODA: "Jisedai Genome Kaiseki Gijutsu - Yotsudomoe no Tatakai no Yukue", BIO TECHNOLOGY JOURNAL, 2007, pages 392 - 393
MIO TOMOUCHI: "Genome Kaiseki no Kakumeiteki Tool: Illumina Genome Analyzer", BIO TECHNOLOGY JOURNAL, vol. 11, 2007, pages 742 - 743
Attorney, Agent or Firm:
OKADA, MASAHIRO (JP)
Patent attorney Masahiro Okada (JP)
Patent attorney Masahiro Okada (JP)
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Claims:
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