Title:
METHOD AND DEVICE FOR FINDING SUBSTANCE MIXTURES FOR SUBSTANCE LIBRARIES
Document Type and Number:
WIPO Patent Application WO2003006152
Kind Code:
A3
Abstract:
The invention relates to a method for finding substance mixtures that are suitable for establishing substance libraries containing low-molecular, organic substances. The inventive method is characterized by preparing the mixtures for analytical purposes, separating the mixtures by chromatography and electrophoresis methods, and identifying the low-molecular, organic substances contained in the respective mixture by establishing a profile from at least two physical parameters for every mixture by detecting every fraction by means of at least two suitable detectors to characterize the substances contained in the mixture, comparing the parameters so obtained with the parameters from a parameter collection of known substances and determining the substances known in said collection for every mixture. The method is specifically characterized in that the new and known substances so identified are quantified, the mixtures in which selected substances are present in a minimum number and/or minimum quantity are selected, the first physical parameter is the retention time and the second physical parameter is the molar mass determined by mass spectrometry.
Inventors:
JAKUPOVIC JASMIN (DE)
BINKELE HEIDRUN (DE)
WOLF DIETMAR (DE)
SIEMS KARSTEN (DE)
BINKELE HEIDRUN (DE)
WOLF DIETMAR (DE)
SIEMS KARSTEN (DE)
Application Number:
PCT/EP2002/007761
Publication Date:
December 18, 2003
Filing Date:
July 11, 2002
Export Citation:
Assignee:
ANALYTICON DISCOVERY GMBH (DE)
JAKUPOVIC JASMIN (DE)
BINKELE HEIDRUN (DE)
WOLF DIETMAR (DE)
SIEMS KARSTEN (DE)
JAKUPOVIC JASMIN (DE)
BINKELE HEIDRUN (DE)
WOLF DIETMAR (DE)
SIEMS KARSTEN (DE)
International Classes:
G16C20/62; G01N30/02; G01N30/74; (IPC1-7): B01J19/00
Other References:
LAMBERT P-H ET AL: "QUALITY ASSESSMENT OF COMBINATORIAL POOLED LIBRARIES USING MASS SPECTROMETRY", COMBINATORIAL CHEMISTRY AND HIGH THROUGHPUT SCREENING, HILVERSUM, NL, vol. 4, no. 4, June 2001 (2001-06-01), pages 317 - 332, XP008017386, ISSN: 1386-2073
HOSTETTMANN K ET AL: "SEARCH FOR NEW LEAD COMPOUNDS FROM HIGHER PLANTS", CHIMIA, AARAU, CH, vol. 54, no. 11, 2000, pages 652 - 657, XP008017376, ISSN: 0009-4293
JIANG Y ET AL: "ON-LINE COUPLING OF HOLLOW FIBER MEMBRANES WITH ELECTROSPRAY IONIZATION MASS SPECTROMETRY FOR CONTINUOUS AFFINITY SELECTION, CONCENTRATION AND IDENTIFICATION OF SMALL-MOLECULE LIBRARIES", JOURNAL OF MASS SPECTROMETRY, WILEY, CHICHESTER, GB, vol. 36, no. 6, 2001, pages 664 - 669, XP008017384, ISSN: 1076-5174
NDJOKO K ET AL: "DETERMINATION OF PYRROLIZIDINE ALKALOIDS IN SENECIO SPECIES BY LIQUID CHROMATOGRAPHY/THERMOSPRAY-MASS SPECTROMETRY AND LIQUID CHROMATOGRAPHY/NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY", PLANTA MEDICA, THIEME, STUTTGART, DE, vol. 65, no. 6, 1999, pages 562 - 566, XP008017380, ISSN: 0032-0943
WOLFENDER J-L ET AL: "THE IMPORTANCE OF LC-MS AND LC-NMR IN THE DISCOVERY OF NEW LEAD COMPOUNDS FROM PLANTS", PHARMACEUTICAL BIOLOGY, SWETS AND ZEITLINGER, LISSE,, NL, vol. 38, no. SUPPL, 2000, pages 41 - 54, XP008017373, ISSN: 1388-0209
FANG L ET AL: "EVALUATION OF EVAPORATIVE LIGHT-SCATTERING DETECTOR FOR COMBINATORIAL LIBRARY QUANTITATION BY REVERSED PHASE HPLC", JOURNAL OF COMBINATORIAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, US, vol. 2, no. 3, May 2000 (2000-05-01), pages 254 - 257, XP001157527, ISSN: 1520-4766
HSU B H ET AL: "APPLICATION OF EVAPORATIVE LIGHT SCATTERING DETECTION TO THE CHARACTERIZATION OF COMBINATORIAL AND PARALLEL SYNTHESIS LIBRARIES FOR PHARMACEUTICAL DRUG DISCOVERY", JOURNAL OF CHROMATOGRAPHY. BIOMEDICAL APPLICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 725, no. 1, 2 April 1999 (1999-04-02), pages 103 - 112, XP001157525, ISSN: 0378-4347
HOSTETTMANN K ET AL: "SEARCH FOR NEW LEAD COMPOUNDS FROM HIGHER PLANTS", CHIMIA, AARAU, CH, vol. 54, no. 11, 2000, pages 652 - 657, XP008017376, ISSN: 0009-4293
JIANG Y ET AL: "ON-LINE COUPLING OF HOLLOW FIBER MEMBRANES WITH ELECTROSPRAY IONIZATION MASS SPECTROMETRY FOR CONTINUOUS AFFINITY SELECTION, CONCENTRATION AND IDENTIFICATION OF SMALL-MOLECULE LIBRARIES", JOURNAL OF MASS SPECTROMETRY, WILEY, CHICHESTER, GB, vol. 36, no. 6, 2001, pages 664 - 669, XP008017384, ISSN: 1076-5174
NDJOKO K ET AL: "DETERMINATION OF PYRROLIZIDINE ALKALOIDS IN SENECIO SPECIES BY LIQUID CHROMATOGRAPHY/THERMOSPRAY-MASS SPECTROMETRY AND LIQUID CHROMATOGRAPHY/NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY", PLANTA MEDICA, THIEME, STUTTGART, DE, vol. 65, no. 6, 1999, pages 562 - 566, XP008017380, ISSN: 0032-0943
WOLFENDER J-L ET AL: "THE IMPORTANCE OF LC-MS AND LC-NMR IN THE DISCOVERY OF NEW LEAD COMPOUNDS FROM PLANTS", PHARMACEUTICAL BIOLOGY, SWETS AND ZEITLINGER, LISSE,, NL, vol. 38, no. SUPPL, 2000, pages 41 - 54, XP008017373, ISSN: 1388-0209
FANG L ET AL: "EVALUATION OF EVAPORATIVE LIGHT-SCATTERING DETECTOR FOR COMBINATORIAL LIBRARY QUANTITATION BY REVERSED PHASE HPLC", JOURNAL OF COMBINATORIAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, US, vol. 2, no. 3, May 2000 (2000-05-01), pages 254 - 257, XP001157527, ISSN: 1520-4766
HSU B H ET AL: "APPLICATION OF EVAPORATIVE LIGHT SCATTERING DETECTION TO THE CHARACTERIZATION OF COMBINATORIAL AND PARALLEL SYNTHESIS LIBRARIES FOR PHARMACEUTICAL DRUG DISCOVERY", JOURNAL OF CHROMATOGRAPHY. BIOMEDICAL APPLICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 725, no. 1, 2 April 1999 (1999-04-02), pages 103 - 112, XP001157525, ISSN: 0378-4347
Download PDF: