Title:
METHOD AND DEVICE FOR ISOLATING SINGLE-STRANDED NUCLEIC ACID, MICROARRAY AND DNA CHIP
Document Type and Number:
WIPO Patent Application WO/2006/093084
Kind Code:
A1
Abstract:
A method for isolating a single-stranded nucleic acid characterized by carrying out nucleic acid amplification using a first primer to which a second substance that can bind specifically to a first substance is bound and a second primer to which the second substance is not bound, allowing a double-stranded nucleic acid obtained by the nucleic acid amplification to bind to the first substance and separating the double-stranded nucleic acid bound to the first substance into single strands; and a device for isolating a single-stranded nucleic acid characterized by comprising a nucleic acid amplifyication unit 1 that carries out nucleic acid amplification using a first primer to which a second substance that can bind specifically to the first substance is bound and a second primer to which the second substance is not bound, a binding unit 2 that allows a double-stranded nucleic acid obtained by the nucleic acid amplification to bind to the first substance, and a separation unit 3 that allows the double-stranded nucleic acid bound to the first substance to separate into single strands.
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Inventors:
MATSUNAGA TADASHI (JP)
TAKEYAMA HARUKO (JP)
TANAKA TSUYOSHI (JP)
TANAAMI TAKEO (JP)
SATO SAYA (JP)
KATAKURA HISAO (JP)
MITSUMORI YUJI (JP)
SHIGEKI GOSUKE (JP)
TAKEYAMA HARUKO (JP)
TANAKA TSUYOSHI (JP)
TANAAMI TAKEO (JP)
SATO SAYA (JP)
KATAKURA HISAO (JP)
MITSUMORI YUJI (JP)
SHIGEKI GOSUKE (JP)
Application Number:
PCT/JP2006/303592
Publication Date:
September 08, 2006
Filing Date:
February 27, 2006
Export Citation:
Assignee:
YOKOGAWA ELECTRIC CORP (JP)
MATSUNAGA TADASHI (JP)
TAKEYAMA HARUKO (JP)
TANAKA TSUYOSHI (JP)
TANAAMI TAKEO (JP)
SATO SAYA (JP)
KATAKURA HISAO (JP)
MITSUMORI YUJI (JP)
SHIGEKI GOSUKE (JP)
MATSUNAGA TADASHI (JP)
TAKEYAMA HARUKO (JP)
TANAKA TSUYOSHI (JP)
TANAAMI TAKEO (JP)
SATO SAYA (JP)
KATAKURA HISAO (JP)
MITSUMORI YUJI (JP)
SHIGEKI GOSUKE (JP)
International Classes:
C12Q1/68; C12M1/00; C12N15/09; G01N33/533; G01N37/00
Domestic Patent References:
| WO1989012063A1 | 1989-12-14 | |||
| WO2004042073A2 | 2004-05-21 |
Foreign References:
| EP0476457A2 | 1992-03-25 | |||
| US20020055146A1 | 2002-05-09 | |||
| EP1208909A2 | 2002-05-29 |
Other References:
HORAI S. ET AL.: "Mitochondria DNA no Bunshi Idengakuteki Kenkyu, Kensaho no Shinpo", JAPANES JOURNAL OF CLINICAL MEDICINE, vol. 51, no. 9, 1993, pages 42 - 47, XP003004046
DEBUIRE B. ET AL.: "Fast, Manual, Nonradioactive Method for DNA Sequencing", CLINICAL CHEMISTRY, vol. 39, no. 8, 1993, pages 1682 - 1685, XP000396972
DEBUIRE B. ET AL.: "Fast, Manual, Nonradioactive Method for DNA Sequencing", CLINICAL CHEMISTRY, vol. 39, no. 8, 1993, pages 1682 - 1685, XP000396972
Attorney, Agent or Firm:
Naito, Teruo (7-13 Nishi-Shimbashi 1-chom, Minato-ku Tokyo 03, JP)
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