Title:
METHOD AND DEVICE FOR THE SYNTHESIS AND THE ANALYSIS OF SUPPORT-BOUND ARRAYS OF OLIGOMERS, ESPECIALLY OF PRIMER PAIRS FOR PCR, AS WELL AS OLIGOMER-CARRYING SUPPORTS
Document Type and Number:
WIPO Patent Application WO2001056691
Kind Code:
A3
Abstract:
The aim of the invention is to synthesize a support-bound array of oligomers with free ends A, said oligomers having a temporary protecting group on the 5'-OH end of the oligomers and permanent protecting groups on the reactive side-groups. To this end, the temporary protecting group is removed from the end B after the combinatorial synthesis of the oligomers onto the support. The free ends B are cross-linked before the permanent protecting groups are removed and the covalent bonds of the synthesized oligomers on the support can be removed via the end A so that free ends A are formed and so that a part of the synthesized oligomers covalently binds to the support even when the free ends A have been removed from the support.
Inventors:
BREITLING FRANK (DE)
POUSTKA ANNEMARIE (DE)
GROSS KARL-HEINZ (DE)
BREITLING FRIEDER (DE)
POUSTKA ANNEMARIE (DE)
GROSS KARL-HEINZ (DE)
BREITLING FRIEDER (DE)
Application Number:
PCT/DE2001/000435
Publication Date:
December 27, 2002
Filing Date:
February 02, 2001
Export Citation:
Assignee:
DEUTSCHES KREBSFORSCH (DE)
BREITLING FRANK (DE)
POUSTKA ANNEMARIE (DE)
GROSS KARL-HEINZ (DE)
BREITLING FRIEDER (DE)
BREITLING FRANK (DE)
POUSTKA ANNEMARIE (DE)
GROSS KARL-HEINZ (DE)
BREITLING FRIEDER (DE)
International Classes:
B01J19/00; C07B61/00; C07H21/00; C12Q1/68; C40B40/06; (IPC1-7): C12Q1/68; C07H21/00
Domestic Patent References:
| WO1998028438A1 | 1998-07-02 | |||
| WO1998036094A1 | 1998-08-20 | |||
| WO1998020020A2 | 1998-05-14 |
Foreign References:
| US5550215A | 1996-08-27 |
Other References:
See also references of EP 1289646A2
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