Title:
METHOD FOR DIFFERENTIAL ASSEMBLY OF HETEROGENEOUS CELL-CONTAINING ASSEMBLY UNITS BY USING FARADAY WAVE, SYSTEM, AND APPLICATION
Document Type and Number:
WIPO Patent Application WO/2023/035768
Kind Code:
A1
Abstract:
Provided are a method for differential assembly of heterogeneous cell-containing assembly units by using a Faraday wave, a system, and an application. The method comprises: adding heterogeneous cell-containing assembly units to a dispersant and mixing well to obtain a suspension system, wherein the heterogeneous cell-containing assembly units satisfy the conditions that: the diameters range from 2 to 4900 μm and/or the buoyant densities range from 1 to 10 g/cm3, and multiple cell-containing assembly units have different diameters and/or buoyant densities; adding the suspension system to an assembly chamber, and after the cell-containing assembly units settle to the bottom of the assembly chamber, performing Faraday wave differential assembly to obtain a cellular microstructure having differentially arranged heterogeneous cells. By adjusting the size and/or buoyancy density of the cell-containing assembly units, real-time differential assembly of the heterogeneous cell-containing assembly units in a Faraday wave sound field is implemented, so that the method is suitable for the requirement for precise differential arrangement of heterogeneous cells in tissue engineering and biomanufacturing, and has great application prospects and technical popularization values.
Inventors:
CHEN PU (CN)
GU LONGJUN (CN)
ZHENG LIXIN (CN)
WANG HENG (CN)
ZHOU JINSHENG (CN)
GU LONGJUN (CN)
ZHENG LIXIN (CN)
WANG HENG (CN)
ZHOU JINSHENG (CN)
Application Number:
PCT/CN2022/105627
Publication Date:
March 16, 2023
Filing Date:
July 14, 2022
Export Citation:
Assignee:
SHENZHEN CONVERGENCE BIO MFG CO LTD (CN)
International Classes:
C12N13/00; C12M1/42; C12M3/02; C12N11/082
Domestic Patent References:
| WO2016069493A2 | 2016-05-06 | |||
| WO2019038453A1 | 2019-02-28 |
Foreign References:
| CN113817715A | 2021-12-21 | |||
| US20160145566A1 | 2016-05-26 |
Other References:
CHEN, P. ET AL.: "Biotunable Acoustic Node Assembly of Organoids", ADVANCED HEALTHCARE MATERIALS, vol. 4, 7 July 2015 (2015-07-07), XP093029597, DOI: 10.1002/adhm.201500279
SERPOOSHAN, V. ET AL.: "Bioacoustic-enabled patterning of human iPSC-derived cardiomyocytes into 3D cardiac tissue", BIOMATERIALS, vol. 131, 28 March 2017 (2017-03-28), pages 47 - 57, XP029991664, DOI: 10.1016/j.biomaterials.2017.03.037
GUEX, A. G. ET AL.: "The waves that make the pattern: a review on acoustic manipulation in biomedical research", PHYSICAL REVIEW E, vol. 10, 24 March 2021 (2021-03-24), XP093029599, DOI: 10.1016/j.mtbio.2021.100110
SHAO, X. ET AL.: "Experimental observation of Faraday waves in soft gels", PHYSICAL REVIEW E, vol. 102, 23 December 2020 (2020-12-23), XP093029602, DOI: 10.1103/PhysRevE.102.060602
SERPOOSHAN, V. ET AL.: "Bioacoustic-enabled patterning of human iPSC-derived cardiomyocytes into 3D cardiac tissue", BIOMATERIALS, vol. 131, 28 March 2017 (2017-03-28), pages 47 - 57, XP029991664, DOI: 10.1016/j.biomaterials.2017.03.037
GUEX, A. G. ET AL.: "The waves that make the pattern: a review on acoustic manipulation in biomedical research", PHYSICAL REVIEW E, vol. 10, 24 March 2021 (2021-03-24), XP093029599, DOI: 10.1016/j.mtbio.2021.100110
SHAO, X. ET AL.: "Experimental observation of Faraday waves in soft gels", PHYSICAL REVIEW E, vol. 102, 23 December 2020 (2020-12-23), XP093029602, DOI: 10.1103/PhysRevE.102.060602
Attorney, Agent or Firm:
WUHAN KEHAO INTELLECTUAL PROPERTY ATTORNEYS(SPECIAL GENERAL PARTNERSHIP) (CN)
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