Title:
METHOD FOR DIFFERENTIATION INDUCTION OF MYOCARDIAL CELL FROM PLURIPOTENT STEM CELL
Document Type and Number:
WIPO Patent Application WO/2007/126077
Kind Code:
A1
Abstract:
The object is to provide a method for differentiation induction of a myocardial cell from a stem cell in a selective manner with high efficiency. Disclosed is a method for differentiation induction of a myocardial cell from a pluripotent stem cell, comprising the following steps (i) and (ii): (i) culturing the pluripotent stem cell in a culture medium containing no substance capable of promoting the activation of the canonical Wnt signaling pathway, wherein the culturing is started at the point of time at which the differentiation induction is initiated and is ended 24 hours before the expression of canonical Wnt gene starts to increase; and (ii) culturing the pluripotent stem cell in a culture medium containing a substance capable of promoting the activation of the canonical Wnt signaling pathway, wherein the culturing is started 24 to 0 hour before the expression of the canonical Wnt gene starts to increase and is continued for 24 to 96 hours.
Inventors:
KOSHIMIZU UICHI (JP)
TANAKA TOMOFUMI (JP)
KAWASHIMA KAYOKO (JP)
KADOKURA MICHINORI (JP)
TANAKA TOMOFUMI (JP)
KAWASHIMA KAYOKO (JP)
KADOKURA MICHINORI (JP)
Application Number:
PCT/JP2007/059242
Publication Date:
November 08, 2007
Filing Date:
April 27, 2007
Export Citation:
Assignee:
ASUBIO PHARMA CO LTD (JP)
KOSHIMIZU UICHI (JP)
TANAKA TOMOFUMI (JP)
KAWASHIMA KAYOKO (JP)
KADOKURA MICHINORI (JP)
KOSHIMIZU UICHI (JP)
TANAKA TOMOFUMI (JP)
KAWASHIMA KAYOKO (JP)
KADOKURA MICHINORI (JP)
International Classes:
C12N5/00; C07K14/47; C12N5/077; A61L27/00; C12N15/09
Domestic Patent References:
| WO2005118782A2 | 2005-12-15 | |||
| WO2000049137A2 | 2000-08-24 | |||
| WO2005033298A1 | 2005-04-14 | |||
| WO2006022377A1 | 2006-03-02 | |||
| WO2003006950A2 | 2003-01-23 | |||
| WO1999027076A1 | 1999-06-03 |
Foreign References:
| US2168763A | 1939-08-08 | |||
| US6015671A | 2000-01-18 | |||
| JP2006023770A | 2006-01-26 | |||
| US5843780A | 1998-12-01 | |||
| US6090622A | 2000-07-18 | |||
| JP2005224155A | 2005-08-25 | |||
| US5340740A | 1994-08-23 | |||
| US5656479A | 1997-08-12 |
Other References:
TSENG A.-S. ET AL.: "The GSK-3 inhibitor BIO promotes proliferation in mammalian cardiomyocytes", CHEM. BIOL., vol. 13, no. 9, September 2006 (2006-09-01), pages 957 - 963, XP005657324
NAITO A.T. ET AL.: "Phosphatidylinositol 3-Kinase-Akt pathway plays as critical role in early cardiomyogenesis by regulating canonical Wnt signaling", CIRC. RES., vol. 97, no. 2, 22 July 2005 (2005-07-22), pages 144 - 151, XP003018975
KOYANAGI M. ET AL.: "Non-canonical Wnt signaling enhances differentiation of human circulating progenitor cells to cardiomyogenic cells", J. BIOL. CHEM., vol. 280, no. 17, 29 April 2005 (2005-04-29), pages 16838 - 16842, XP003018976
TERAMI H. ET AL.: "Wnt11 facilitates embryonic stem cell differentiation to Nkx2.5-positive cardiomyocytes", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 325, no. 3, 17 December 2004 (2004-12-17), pages 968 - 975, XP004635519
YUASA S. ET AL.: "Transient inhibition of BMP signaling by Noggin induces cardiomyocyte differentiation of mouse embryonic stem cells", NAT. BIOTECHNOL., vol. 23, no. 5, May 2005 (2005-05-01), pages 607 - 611, XP002421916
SUEMORI ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 345, 2006, pages 926
MITALIPOVA ET AL., CLONING, vol. 3, 2001, pages 59
PETITTE ET AL., MECH. DEV., vol. 121, 2004, pages 1159
FISHMAN, SCIENCE, vol. 294, 2001, pages 1290
MUNSIE ET AL., CURR. BIOL., vol. 10, 2000, pages 989
WAKAYAMA ET AL., SCIENCE, vol. 292, 2001, pages 740
HWANG ET AL., SCIENCE, vol. 303, 2004, pages 1669
VRANA K ET AL., PROC. NATL. ACAD. SCI. USA, vol. 100, pages 11911 - 6
TADA ET AL., CURR. BIOL., vol. 11, 2001, pages 1553
WILLERT ET AL., NATURE, vol. 423, 2003, pages 448
KISHIDA ET AL., MOL. CELL. BIOL., vol. 24, pages 4487, Retrieved from the Internet
MARTINEZ ET AL., MED. RES. REV., vol. 22, 2002, pages 373
MEIJER L ET AL., TRENDS PHARMACOL. SCI., vol. 25, 2004, pages 471
LIU ET AL., ANGEW. CHEM. INT. ED. ENGL., vol. 44, 2005, pages 1987
YUASA ET AL., NAT. BIOTECHNOL., vol. 23, 2005, pages 607
BEQQALI ET AL., STEM CELLS, vol. 24, 2006, pages 1956
"Monoclonal Antibodies: principles and practice, Third Edition", 1993, ACAD. PRESS
"Antibody Engineering: A Practical Approach", 1996, IRL PRESS AT OXFORD UNIVERSITY PRESS
KLUG ET AL., J. CLIN. INVEST., vol. 98, 1996, pages 216
SHIMIZU ET AL., CIRC. RES., vol. 90, 2002, pages E40
MURRY ET AL., COLD SPRING HARB. SYMP. QUANT. BIOL., vol. 67, 2002, pages 519
MENASCHE, ANN. THORAC. SURG., vol. 75, 2003, pages S20
DOWELL ET AL., CARDIOVASC. RES., vol. 58, 2003, pages 336
REFFELMANN ET AL., HEART FAIL. REV., vol. 8, 2003, pages 201
MALTSEV ET AL., MECH. DEV., vol. 44, 1993, pages 41
CIRC. RES., vol. 75, 1994, pages 233
DOEVENDANS ET AL., J. MOL. CELL. CARDIOL., vol. 32, 2000, pages 839
LAFLAMME ET AL., AM. J. PATHOL., vol. 167, pages 663
"Manipulating the Mouse Embryo: A Laboratory Manual", 1994, COLD SPRING HARBOR LABORATORY PRESS
"Embryonic Stem Cells: Methods and Protocols", 2002, HUMANA PRESS
SOONPAA MH ET AL., SCIENCE, vol. 264, 1994, pages 98
MALTSEV VA ET AL., MECHANISM OF DEVELOPMENT, vol. 44, 1993, pages 41
MALTSEV VA ET AL., CIRCULATION RESEARCH, vol. 75, 1994, pages 233
KLUG MG ET AL., JOURNAL OF CLINICAL INVESTIGATION, vol. 98, 1996, pages 216
THOMSON JA ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 92, 1995, pages 7844
THOMSON JA ET AL., SCIENCE, vol. 282, 1998, pages 1145
SHAMBLOTT MJ ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 95, 1998, pages 13726
KEHAT I ET AL., JOURNAL OF CLINICAL INVESTIGATION, vol. 108, 2001, pages 407
XU C ET AL., CIRCULATION RESEARCH, vol. 91, 2002, pages 501
WOBUS AM ET AL., JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, vol. 29, 1997, pages 1525
TAKAHASHI T ET AL., CIRCULATION, vol. 107, 2003, pages 1912
BEHFAR A ET AL., FASEB JOURNAL, vol. 16, 2002, pages 1558
SACHINIDIS ET AL., CARDIOVASCULAR RESEARCH, vol. 58, 2003, pages 278
VENTURA C ET AL., CIRCULATION RESEARCH, vol. 92, 2003, pages 623
SAUER H ET AL., FEBS LETTERS, vol. 476, 2000, pages 218
LI J ET AL., JOURNAL OF CELL BIOLOGY, vol. 158, 2002, pages 103
YUASA S ET AL., NATURE BIOTECHNOLOGY, vol. 23, 2005, pages 607
NUSSE R, CELL RESEARCH, vol. 15, 2005, pages 28
WIDELITZ R, GROWTH FACTORS, vol. 23, 2005, pages 111
KÜHL M ET AL., TRENDS IN GENETICS, vol. 16, 2000, pages 279
HAO J ET AL., DEVELOPMENTAL BIOLOGY, vol. 290, 2006, pages 81
SCHNEIDER VA; MERCOLA M, GENES AND DEVELOPMENT, vol. 15, 2001, pages 304
MARVIN MJ ET AL., GENES AND DEVELOPMENT, vol. 15, 2001, pages 316
PANDUR P ET AL., NATURE, vol. 418, 2002, pages 636
TERAMI H ET AL., BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATION, vol. 325, 2004, pages 968
KOYANAGI M ET AL., JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 280, 2005, pages 16838
NAKAMURA T ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 100, 2003, pages 5834
NAITO AT ET AL., CIRCULATION RESEARCH, vol. 97, 2005, pages 144
YAMASHITA JK ET AL., FASEB JOURNAL, vol. 19, 2002, pages 1534
NAITO AT ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 103, 2006, pages 19812
SAMBROOK; RUSSELL: "Molecular Cloning: A Laboratory Manual, Third Edition", 2001, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular biology", 1987, JOHN WILEY & SONS
"Methods in Enzymology series", ACADEMIC PRESS
"PCR Protocols: Methods in Molecular Biology", 2003, HUMANA PRESS
"Animal Cell Culture: A Practical Approach, Third Edition", 2000, OXFORD UNIVERSITY PRESS
"Antibodies: A Laboratory Manual", 1987, COLD SPRING HARBOR LABORATORY PRESS
"Guide to Techniques in Mouse Development", 1993, ACADEMIC PRESS
M.V. WILES: "Embryonic Stem Cell Differentiation in vitro", METH. ENZYMOL., vol. 225, 1993, pages 900, XP009097134, DOI: doi:10.1016/0076-6879(93)25057-9
"Manipulating the Mouse Embryo: A laboratory manual", 1994, COLD SPRING HARBOR LABORATORY PRESS
"Embryonic Stem Cells", 2002, HUMANA PRESS
MATSUI ET AL., CELL, vol. 70, 1992, pages 841
THOMSON ET AL., SCIENCE, vol. 282, 1998, pages 114
SHAMBLOTT ET AL., PROC. NATL. ACAD. SCI. USA, vol. 95, 1998, pages 13726
REUBINOFF ET AL., NAT. BIOTECH., vol. 18, 2000, pages 399
PROC. NATL. ACAD. SCI. USA, vol. 92, 1996, pages 7844
LANNACCONE ET AL., DEV. BIOL., vol. 163, 1994, pages 288
PAIN ET AL., DEVELOPMENT, vol. 122, 1996, pages 2339
WHEELER ET AL., REPROD. FERTIL. DEV., vol. 6, 1994, pages 563
SHIM ET AL., BIOL. REPROD., vol. 57, 1997, pages 1089
THOMSON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 92, 1995, pages 7844
SUEMORI ET AL., DEV. DYN., vol. 222, 2001, pages 273
SASAKI ET AL., STEM CELLS., vol. 23, 2005, pages 1304
See also references of EP 2014766A4
NAITO A.T. ET AL.: "Phosphatidylinositol 3-Kinase-Akt pathway plays as critical role in early cardiomyogenesis by regulating canonical Wnt signaling", CIRC. RES., vol. 97, no. 2, 22 July 2005 (2005-07-22), pages 144 - 151, XP003018975
KOYANAGI M. ET AL.: "Non-canonical Wnt signaling enhances differentiation of human circulating progenitor cells to cardiomyogenic cells", J. BIOL. CHEM., vol. 280, no. 17, 29 April 2005 (2005-04-29), pages 16838 - 16842, XP003018976
TERAMI H. ET AL.: "Wnt11 facilitates embryonic stem cell differentiation to Nkx2.5-positive cardiomyocytes", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 325, no. 3, 17 December 2004 (2004-12-17), pages 968 - 975, XP004635519
YUASA S. ET AL.: "Transient inhibition of BMP signaling by Noggin induces cardiomyocyte differentiation of mouse embryonic stem cells", NAT. BIOTECHNOL., vol. 23, no. 5, May 2005 (2005-05-01), pages 607 - 611, XP002421916
SUEMORI ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 345, 2006, pages 926
MITALIPOVA ET AL., CLONING, vol. 3, 2001, pages 59
PETITTE ET AL., MECH. DEV., vol. 121, 2004, pages 1159
FISHMAN, SCIENCE, vol. 294, 2001, pages 1290
MUNSIE ET AL., CURR. BIOL., vol. 10, 2000, pages 989
WAKAYAMA ET AL., SCIENCE, vol. 292, 2001, pages 740
HWANG ET AL., SCIENCE, vol. 303, 2004, pages 1669
VRANA K ET AL., PROC. NATL. ACAD. SCI. USA, vol. 100, pages 11911 - 6
TADA ET AL., CURR. BIOL., vol. 11, 2001, pages 1553
WILLERT ET AL., NATURE, vol. 423, 2003, pages 448
KISHIDA ET AL., MOL. CELL. BIOL., vol. 24, pages 4487, Retrieved from the Internet
MARTINEZ ET AL., MED. RES. REV., vol. 22, 2002, pages 373
MEIJER L ET AL., TRENDS PHARMACOL. SCI., vol. 25, 2004, pages 471
LIU ET AL., ANGEW. CHEM. INT. ED. ENGL., vol. 44, 2005, pages 1987
YUASA ET AL., NAT. BIOTECHNOL., vol. 23, 2005, pages 607
BEQQALI ET AL., STEM CELLS, vol. 24, 2006, pages 1956
"Monoclonal Antibodies: principles and practice, Third Edition", 1993, ACAD. PRESS
"Antibody Engineering: A Practical Approach", 1996, IRL PRESS AT OXFORD UNIVERSITY PRESS
KLUG ET AL., J. CLIN. INVEST., vol. 98, 1996, pages 216
SHIMIZU ET AL., CIRC. RES., vol. 90, 2002, pages E40
MURRY ET AL., COLD SPRING HARB. SYMP. QUANT. BIOL., vol. 67, 2002, pages 519
MENASCHE, ANN. THORAC. SURG., vol. 75, 2003, pages S20
DOWELL ET AL., CARDIOVASC. RES., vol. 58, 2003, pages 336
REFFELMANN ET AL., HEART FAIL. REV., vol. 8, 2003, pages 201
MALTSEV ET AL., MECH. DEV., vol. 44, 1993, pages 41
CIRC. RES., vol. 75, 1994, pages 233
DOEVENDANS ET AL., J. MOL. CELL. CARDIOL., vol. 32, 2000, pages 839
LAFLAMME ET AL., AM. J. PATHOL., vol. 167, pages 663
"Manipulating the Mouse Embryo: A Laboratory Manual", 1994, COLD SPRING HARBOR LABORATORY PRESS
"Embryonic Stem Cells: Methods and Protocols", 2002, HUMANA PRESS
SOONPAA MH ET AL., SCIENCE, vol. 264, 1994, pages 98
MALTSEV VA ET AL., MECHANISM OF DEVELOPMENT, vol. 44, 1993, pages 41
MALTSEV VA ET AL., CIRCULATION RESEARCH, vol. 75, 1994, pages 233
KLUG MG ET AL., JOURNAL OF CLINICAL INVESTIGATION, vol. 98, 1996, pages 216
THOMSON JA ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 92, 1995, pages 7844
THOMSON JA ET AL., SCIENCE, vol. 282, 1998, pages 1145
SHAMBLOTT MJ ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 95, 1998, pages 13726
KEHAT I ET AL., JOURNAL OF CLINICAL INVESTIGATION, vol. 108, 2001, pages 407
XU C ET AL., CIRCULATION RESEARCH, vol. 91, 2002, pages 501
WOBUS AM ET AL., JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, vol. 29, 1997, pages 1525
TAKAHASHI T ET AL., CIRCULATION, vol. 107, 2003, pages 1912
BEHFAR A ET AL., FASEB JOURNAL, vol. 16, 2002, pages 1558
SACHINIDIS ET AL., CARDIOVASCULAR RESEARCH, vol. 58, 2003, pages 278
VENTURA C ET AL., CIRCULATION RESEARCH, vol. 92, 2003, pages 623
SAUER H ET AL., FEBS LETTERS, vol. 476, 2000, pages 218
LI J ET AL., JOURNAL OF CELL BIOLOGY, vol. 158, 2002, pages 103
YUASA S ET AL., NATURE BIOTECHNOLOGY, vol. 23, 2005, pages 607
NUSSE R, CELL RESEARCH, vol. 15, 2005, pages 28
WIDELITZ R, GROWTH FACTORS, vol. 23, 2005, pages 111
KÜHL M ET AL., TRENDS IN GENETICS, vol. 16, 2000, pages 279
HAO J ET AL., DEVELOPMENTAL BIOLOGY, vol. 290, 2006, pages 81
SCHNEIDER VA; MERCOLA M, GENES AND DEVELOPMENT, vol. 15, 2001, pages 304
MARVIN MJ ET AL., GENES AND DEVELOPMENT, vol. 15, 2001, pages 316
PANDUR P ET AL., NATURE, vol. 418, 2002, pages 636
TERAMI H ET AL., BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATION, vol. 325, 2004, pages 968
KOYANAGI M ET AL., JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 280, 2005, pages 16838
NAKAMURA T ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 100, 2003, pages 5834
NAITO AT ET AL., CIRCULATION RESEARCH, vol. 97, 2005, pages 144
YAMASHITA JK ET AL., FASEB JOURNAL, vol. 19, 2002, pages 1534
NAITO AT ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 103, 2006, pages 19812
SAMBROOK; RUSSELL: "Molecular Cloning: A Laboratory Manual, Third Edition", 2001, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular biology", 1987, JOHN WILEY & SONS
"Methods in Enzymology series", ACADEMIC PRESS
"PCR Protocols: Methods in Molecular Biology", 2003, HUMANA PRESS
"Animal Cell Culture: A Practical Approach, Third Edition", 2000, OXFORD UNIVERSITY PRESS
"Antibodies: A Laboratory Manual", 1987, COLD SPRING HARBOR LABORATORY PRESS
"Guide to Techniques in Mouse Development", 1993, ACADEMIC PRESS
M.V. WILES: "Embryonic Stem Cell Differentiation in vitro", METH. ENZYMOL., vol. 225, 1993, pages 900, XP009097134, DOI: doi:10.1016/0076-6879(93)25057-9
"Manipulating the Mouse Embryo: A laboratory manual", 1994, COLD SPRING HARBOR LABORATORY PRESS
"Embryonic Stem Cells", 2002, HUMANA PRESS
MATSUI ET AL., CELL, vol. 70, 1992, pages 841
THOMSON ET AL., SCIENCE, vol. 282, 1998, pages 114
SHAMBLOTT ET AL., PROC. NATL. ACAD. SCI. USA, vol. 95, 1998, pages 13726
REUBINOFF ET AL., NAT. BIOTECH., vol. 18, 2000, pages 399
PROC. NATL. ACAD. SCI. USA, vol. 92, 1996, pages 7844
LANNACCONE ET AL., DEV. BIOL., vol. 163, 1994, pages 288
PAIN ET AL., DEVELOPMENT, vol. 122, 1996, pages 2339
WHEELER ET AL., REPROD. FERTIL. DEV., vol. 6, 1994, pages 563
SHIM ET AL., BIOL. REPROD., vol. 57, 1997, pages 1089
THOMSON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 92, 1995, pages 7844
SUEMORI ET AL., DEV. DYN., vol. 222, 2001, pages 273
SASAKI ET AL., STEM CELLS., vol. 23, 2005, pages 1304
See also references of EP 2014766A4
Attorney, Agent or Firm:
SHAMOTO, Ichio et al. (Section 206 New Ohtemachi Bldg.,2-1, Ohtemachi 2-chom, Chiyoda-ku Tokyo 04, JP)
Download PDF:
Previous Patent: CISPLATIN RESISTANCE MARKER FOR OVARIAN TUMOR
Next Patent: BAG HAVING THREE-DIMENSIONAL DESIGN
Next Patent: BAG HAVING THREE-DIMENSIONAL DESIGN