METHOD FOR DILUTE PLASMA SEPARATION USING CONTAINER FOR BLOOD DILUTION AND STORAGE CONTAINING GELLING AGENT FOR PLASMA SEPARATION

Title:

METHOD FOR DILUTE PLASMA SEPARATION USING CONTAINER FOR BLOOD DILUTION AND STORAGE CONTAINING GELLING AGENT FOR PLASMA SEPARATION

Document Type and Number:

WIPO Patent Application WO/2015/189961

Kind Code:

A1

Abstract:

Provided is a method for separating plasma in a sample obtained by diluting a very small amount of blood with a blood dilution buffer-solution. When a blood dilution buffer is added to a very small amount of blood of 20 to 100 µL and the biological components in the diluted plasma are to be assayed, the diluted blood cell component and the diluted plasma component must be stored separately to be transported. In the present invention, it was discovered that a polymer gelling agent for plasma separation can separate the diluted plasma from diluted blood cells and plasma when a conventional polymer gel for plasma separation is introduced to a container containing a blood dilution buffer-solution. The diluted blood cells will separate to below the polymer gelling agent and the diluted plasma will separate to above the gelling agent. The method can be used to assay the biological components in blood using a very small amount of blood for determining health status, early detection of disease, and disease prevention. The method has an advantage in that blood can be collected independent of time and place.

Inventors:

SUGIMOTO SHINYA (JP)
YONEKUBO ISAO (JP)
KAWAGUCHI MASATO (JP)
OSAWA SUSUMU (JP)

Application Number:

PCT/JP2014/065628

Publication Date:

December 17, 2015

Filing Date:

June 12, 2014

Export Citation:

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Assignee:

LEISURE INC (JP)

International Classes:

G01N33/48

Foreign References:

JP2003294731A	2003-10-15
JPH02134564A	1990-05-23
JPH08201391A	1996-08-09
JPH08108096A	1996-04-30
JP2001255323A	2001-09-21

Other References: