Title:
METHOD FOR DIRECT QUANTIFICATION OF MULTIPLE APOLIPOPROTEINS IN SERUM
Document Type and Number:
WIPO Patent Application WO/2024/014865
Kind Code:
A1
Abstract:
The present invention relates to a method for isolating and/or detecting apolipoprotein from a biological sample by preprocessing with an organic solvent. Additionally, the invention relates to a matrix and internal standard composition for mass analysis of proteins including sera from ruminant animals. The present invention not only allows direct quantification of various proteoforms even without enzymatic cleavage, but also uses animal serum as a substrate to simply maintain the matrix of the sample without removing the target protein in the sample, and the objectivity and accuracy of quantitative results were ensured at minimal cost by using proteins in animal serum as internal standards instead of existing synthetic peptides or synthetic proteins that requires time and cost for their synthesis. Thus, the present invention can be advantageously used as an efficient analytical method that enables the extraction of target proteins with high purity from samples and provides highly reliable quantitative information on individual proteoforms with various glycosylation patterns, as well as information on the total amount of all proteoforms in the sample.
Inventors:
CHUN JONG KEE (KR)
BAEK JE HYUN (KR)
YANG WON SUK (KR)
KIM HYOJIN (KR)
CHEON DONG HUEY (KR)
BAEK JE HYUN (KR)
YANG WON SUK (KR)
KIM HYOJIN (KR)
CHEON DONG HUEY (KR)
Application Number:
PCT/KR2023/009932
Publication Date:
January 18, 2024
Filing Date:
July 12, 2023
Export Citation:
Assignee:
SEEGENE MEDICAL FOUND (KR)
International Classes:
G01N1/40; G01N27/623; G01N30/72; G01N33/68; G01N33/92
Foreign References:
| KR20140053848A | 2014-05-08 | |||
| US9810702B2 | 2017-11-07 |
Other References:
PEPAJ MILAIM, LUNDANES ELSA, GREIBROKK TYGE: "Separation of Apolipoprotein A‐I from Human Plasma by On‐Line Two Dimensional Liquid Chromatography", JOURNAL OF LIQUID CHROMATOGRAPHY AND RELATED TECHNOLOGIES, MONTICELLO, NY, US, vol. 30, no. 13, 1 May 2007 (2007-05-01), US , pages 1879 - 1894, XP093129608, ISSN: 1082-6076, DOI: 10.1080/10826070701386439
LEESA J. DETERDING; JENNY M. CUTALO; MORTEZA KHALEDI; KENNETH B. TOMER: "Separation and characterization of human high‐density apolipoproteins using a nonaqueous modifier in capillary electrophoresis‐mass spectrometry", ELECTROPHORESIS, VERLAG CHEMIE, HOBOKEN, USA, vol. 23, no. 14, 23 July 2002 (2002-07-23), Hoboken, USA, pages 2296 - 2305, XP071496160, ISSN: 0173-0835, DOI: 10.1002/1522-2683(200207)23:14<2296::AID-ELPS2296>3.0.CO;2-I
TETAZ, T. ; KECORIUS, E. ; GREGO, B. ; FIDGE, N.: "Separation of human aplipoproteins A-IV, A-I and E by reversed-phase high-performance liquid chromatography on a TSK Phenyl-5PW column", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER, AMSTERDAM, NL, vol. 511, 1 January 1990 (1990-01-01), AMSTERDAM, NL, pages 147 - 153, XP026549967, ISSN: 0021-9673, DOI: 10.1016/S0021-9673(01)93280-7
LEESA J. DETERDING; JENNY M. CUTALO; MORTEZA KHALEDI; KENNETH B. TOMER: "Separation and characterization of human high‐density apolipoproteins using a nonaqueous modifier in capillary electrophoresis‐mass spectrometry", ELECTROPHORESIS, VERLAG CHEMIE, HOBOKEN, USA, vol. 23, no. 14, 23 July 2002 (2002-07-23), Hoboken, USA, pages 2296 - 2305, XP071496160, ISSN: 0173-0835, DOI: 10.1002/1522-2683(200207)23:14<2296::AID-ELPS2296>3.0.CO;2-I
TETAZ, T. ; KECORIUS, E. ; GREGO, B. ; FIDGE, N.: "Separation of human aplipoproteins A-IV, A-I and E by reversed-phase high-performance liquid chromatography on a TSK Phenyl-5PW column", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER, AMSTERDAM, NL, vol. 511, 1 January 1990 (1990-01-01), AMSTERDAM, NL, pages 147 - 153, XP026549967, ISSN: 0021-9673, DOI: 10.1016/S0021-9673(01)93280-7
Attorney, Agent or Firm:
PADO IP LAW PLLC (KR)
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