Title:
METHOD OF DISTINGUISHING MESENCHYMAL STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2016/086403
Kind Code:
A1
Abstract:
A method of distinguishing mesenchymal stem cells (MSCs) in a primary culture of cells derived from a placenta-related tissue is provided. Also provided is a method of increasing the purity of MSC population in a primary culture of cells derived from a placenta-related tissue. Further provided is a method of isolating a MSC population which is more responsive in an inflammatory environment. Said methods each comprise a step of sorting the cells by a marker of EphA2.
Inventors:
HSUAN CHANG-YO (CN)
LIU WEI-TING (CN)
SHEN SHIH-PEI (CN)
LIN YUN (CN)
LI YUAN-TSUNG (CN)
CHANG CHIA-CHI (CN)
LIU WEI-TING (CN)
SHEN SHIH-PEI (CN)
LIN YUN (CN)
LI YUAN-TSUNG (CN)
CHANG CHIA-CHI (CN)
Application Number:
PCT/CN2014/093109
Publication Date:
June 09, 2016
Filing Date:
December 05, 2014
Export Citation:
Assignee:
MERIDIGEN BIOTECH CO LTD (CN)
International Classes:
G01N33/536; C12N5/0775; C12Q1/68
Domestic Patent References:
| WO2005005601A2 | 2005-01-20 |
Other References:
TATIANA TAIS SIBOV?P ET AL.: "Mesenchymal stem cells from umbilical cord blood: parameters for isolation, characterization and adipogenic differentiation", CYTOTECHNOLOGY, vol. 64, no. 5, 31 October 2012 (2012-10-31), pages 511 - 521, XP055220346, ISSN: 0920-9069, DOI: doi:10.1007/s10616-012-9428-3
TATIANA TAÎS SIBOV?P ET AL.: "Mesenchymal stem cells from umbilical cord blood: parameters for isolation, characterization and adipogenic differentiation", CYTOTECHNOLOGY, vol. 64, no. 5, 31 October 2012 (2012-10-31), pages 511 - 521, XP055220346, ISSN: 0920-9069, DOI: doi:10.1007/s10616-012-9428-3
DAI, NA ET AL.: "Influence of Vascular Endothelial Growth Factor on Endothelial Components in Human Bone Marrrow and Umbilical Cord Mesenchymal Stem Cells", JOURNAL OF EXPERIMENTAL HEMATOLOGY, vol. 20, no. 3, 31 December 2012 (2012-12-31), pages 717 - 721, XP008185476, ISSN: 1009-2137
See also references of EP 3227682A4
TATIANA TAÎS SIBOV?P ET AL.: "Mesenchymal stem cells from umbilical cord blood: parameters for isolation, characterization and adipogenic differentiation", CYTOTECHNOLOGY, vol. 64, no. 5, 31 October 2012 (2012-10-31), pages 511 - 521, XP055220346, ISSN: 0920-9069, DOI: doi:10.1007/s10616-012-9428-3
DAI, NA ET AL.: "Influence of Vascular Endothelial Growth Factor on Endothelial Components in Human Bone Marrrow and Umbilical Cord Mesenchymal Stem Cells", JOURNAL OF EXPERIMENTAL HEMATOLOGY, vol. 20, no. 3, 31 December 2012 (2012-12-31), pages 717 - 721, XP008185476, ISSN: 1009-2137
See also references of EP 3227682A4
Attorney, Agent or Firm:
GE CHENG & CO., LTD. (Tower E3 The Towers, Oriental Plaza, No.1, East Chang An Avenue,Dongcheng District, Beijing 8, CN)
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