Title:
METHOD FOR EDITING FILAMENTOUS FUNGAL GENOME THROUGH DIRECT INTRODUCTION OF GENOME-EDITING PROTEIN
Document Type and Number:
WIPO Patent Application WO/2018/123134
Kind Code:
A1
Abstract:
The present invention provides a method for editing a filamentous fungal genome through direct introduction of a genome-editing protein molecule or complex. Said method includes three aspects. According to the first aspect, a genome-editing protein molecule or complex for a target gene is directly introduced into an Aspergillus fungal cell to edit a gene in the Aspergillus fungal genome. According to the second aspect, a desired DNA fragment and a genome-editing protein molecule or complex for a target region in the filamentous fungal genome is directly introduced into a filamentous fungal cell to knock-in the DNA fragment at a desired target site in the filamentous fungal genome. According to the third aspect, genome-editing protein molecules or complexes for multiple target genes are directly introduced into a filamentous fungal cell to co-edit the multiple genes in the filamentous fungal genome.
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Inventors:
IWASHITA KAZUHIRO (JP)
ODA KEN (JP)
SHIMAMOTO KOHEI (JP)
WADA YUSAKU (JP)
ODA KEN (JP)
SHIMAMOTO KOHEI (JP)
WADA YUSAKU (JP)
Application Number:
PCT/JP2017/030501
Publication Date:
July 05, 2018
Filing Date:
August 25, 2017
Export Citation:
Assignee:
NATIONAL RES INSTITUTE OF BREWING (JP)
FASMAC CO LTD (JP)
FASMAC CO LTD (JP)
International Classes:
C12N15/09; C12N1/15
Domestic Patent References:
| WO2015054507A1 | 2015-04-16 |
Other References:
POHL, C. ET AL.: "CRISPR/Cas9 Based Genome Editing of Penicillium chrysogenum", ACS SYNTHETIC BIOLOGY, vol. 5, 15 July 2016 (2016-07-15), pages 754 - 764, XP055390602
KATAYAMA, TAKUYA ET AL.: "Development of a genome editing technique using the CRISPR/Cas9 system in the industrial filamentous fungus Aspergillus oryzae", BIOTECHNOLOGY LETTERS, vol. 38, 19 December 2015 (2015-12-19), pages 637 - 642, XP035901398, [retrieved on 20151219]
FULLER, KEVIN K. ET AL.: "Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus", EUKARYOTIC CELL, vol. 14, no. 11, 1 November 2015 (2015-11-01), pages 1073 - 1080, XP002755315
NODVIG, CHRISTINA S. ET AL.: "A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi", PLOS ONE, vol. 10, 15 July 2015 (2015-07-15), pages 1 - 18, XP055256394
ZHANG, CHI ET AL.: "Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus", FUNGAL GENETICS AND BIOLOGY, vol. 86, 14 December 2015 (2015-12-14), pages 47 - 57, XP055256363
SANDER, JEFFRY D. ET AL.: "CRISPR-Cas systems for editing, regulating and targeting genomes", NATURE BIOTECHNOLOGY., vol. 32, no. 4, 1 April 2014 (2014-04-01), pages 347 - 355, XP055481941
SHIMAMOTO, K. ET AL.: "Positive Selection ga Dekinai Idenshi no Kojikin Genome Henshuho no Kaihatsu", PROCEEDINGS OF THE 2017 ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECNOLOGY AND AGROCHEMISTRY, 5 March 2017 (2017-03-05)
SHIMAMOTO, K. ET AL.: "Cas9 tanpakushitsu o Mochiita Konjikin no Genome Henshu Gijutsu no Kaihatsu", ABSTRACTS OF THE 68TH ANNUAL MEETING OF THE SOCIETY BIOTECHNOLOGY, JAPAN, vol. 68, 25 August 2016 (2016-08-25), pages 279
KATAYAMA, TAKUYA ET AL.: "Development of a genome editing technique using the CRISPR/Cas9 system in the industrial filamentous fungus Aspergillus oryzae", BIOTECHNOLOGY LETTERS, vol. 38, 19 December 2015 (2015-12-19), pages 637 - 642, XP035901398, [retrieved on 20151219]
FULLER, KEVIN K. ET AL.: "Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus", EUKARYOTIC CELL, vol. 14, no. 11, 1 November 2015 (2015-11-01), pages 1073 - 1080, XP002755315
NODVIG, CHRISTINA S. ET AL.: "A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi", PLOS ONE, vol. 10, 15 July 2015 (2015-07-15), pages 1 - 18, XP055256394
ZHANG, CHI ET AL.: "Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus", FUNGAL GENETICS AND BIOLOGY, vol. 86, 14 December 2015 (2015-12-14), pages 47 - 57, XP055256363
SANDER, JEFFRY D. ET AL.: "CRISPR-Cas systems for editing, regulating and targeting genomes", NATURE BIOTECHNOLOGY., vol. 32, no. 4, 1 April 2014 (2014-04-01), pages 347 - 355, XP055481941
SHIMAMOTO, K. ET AL.: "Positive Selection ga Dekinai Idenshi no Kojikin Genome Henshuho no Kaihatsu", PROCEEDINGS OF THE 2017 ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECNOLOGY AND AGROCHEMISTRY, 5 March 2017 (2017-03-05)
SHIMAMOTO, K. ET AL.: "Cas9 tanpakushitsu o Mochiita Konjikin no Genome Henshu Gijutsu no Kaihatsu", ABSTRACTS OF THE 68TH ANNUAL MEETING OF THE SOCIETY BIOTECHNOLOGY, JAPAN, vol. 68, 25 August 2016 (2016-08-25), pages 279
Attorney, Agent or Firm:
TANIGAWA AND PARTNERS, PATENT FIRM (JP)
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