Title:
METHOD FOR EFFICIENTLY AND STABLY TRANSDUCING ANTIGEN CHIMERIC RECEPTOR DOUBLE-NEGATIVE T CELLS IN VITRO
Document Type and Number:
WIPO Patent Application WO/2023/078425
Kind Code:
A1
Abstract:
The invention provides a safe and efficient method for in vitro transduction of double-negative T cells (DNT), and cells prepared using the method, and a preparation containing the cells. In the present invention, under the condition that a transduction-assisting cationic reagent is not added, stable and efficient transduction efficiency can be obtained using fresh enriched or cryopreserved recovered double-negative T cells which are activated and amplified by lentivirus transduction. A chimeric antigen receptor - double-negative T cell (CAR-DNT) prepared using the method of the present invention not only has higher cell viability and purity, but also maintains high tumor-killing activity, and avoids the high residual hidden dangers caused by adding a cationic transduction reagent during CAR-DNT cell production.
Inventors:
YANG LIMING (CN)
WANG DAN (CN)
WANG LIUYANG (CN)
ZHU HONGLIN (CN)
XU MAN (CN)
WANG DAN (CN)
WANG LIUYANG (CN)
ZHU HONGLIN (CN)
XU MAN (CN)
Application Number:
PCT/CN2022/130037
Publication Date:
May 11, 2023
Filing Date:
November 04, 2022
Export Citation:
Assignee:
ZHEJIANG RUIJIAMEI BIOTECH CO LTD (CN)
International Classes:
A61K39/00; C12N5/10; A61P35/00; C07K19/00; C12N15/62; C12N15/867
Domestic Patent References:
| WO2021178975A1 | 2021-09-10 |
Foreign References:
| CN113528449A | 2021-10-22 | |||
| CN104789595A | 2015-07-22 | |||
| CN107567461A | 2018-01-09 |
Other References:
LIU, HAN ET AL.: "Research Progress of Double Negative T Cells", HEBEI MEDICAL JOURNAL, vol. 35, no. 12, 26 June 2013 (2013-06-26), XP009545943, ISSN: 1002-7386
YAO, JUNLIN ET AL.: "Human double negative T cells target lung cancer via ligand-dependent mechanisms that can be enhanced by IL-15", THE JOURNAL FOR IMMUNOTHERAPY OF CANCER, vol. 7, no. 1, 22 January 2019 (2019-01-22), XP021270879, ISSN: 2051-1426, DOI: 10.1186/s40425-019-0507-2
YAO, JUNLIN ET AL.: "Human double negative T cells target lung cancer via ligand-dependent mechanisms that can be enhanced by IL-15", THE JOURNAL FOR IMMUNOTHERAPY OF CANCER, vol. 7, no. 1, 22 January 2019 (2019-01-22), XP021270879, ISSN: 2051-1426, DOI: 10.1186/s40425-019-0507-2
Attorney, Agent or Firm:
XU&PARTNERS, LLC. (CN)
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