Title:
METHOD FOR THE ELECTRICAL DETECTION OF PEPTIDES, PROTEINS AND OTHER MACROMOLECULES
Document Type and Number:
WIPO Patent Application WO/2017/220875
Kind Code:
A3
Abstract:
The invention relates to the use of an aerolysin nanopore or a nanotube for the electrical detection of peptides, different proteins of at least one amino acid and other macromolecules, such as polysaccharides or synthetic or natural polymers present in a preparation, in which the nanopore or nanotube is inserted into a lipid membrane that is subjected to a potential difference greater than -160mV, in a reaction medium comprising an alkali metal halide electrolyte solution having a concentration of less than 6M and a temperature of less than 40°C, for the purpose of differentiating the aforementioned peptides, proteins and other molecules according to length and mass.
Inventors:
PELTA JUAN (FR)
OUKHALED ABDELGHANI (FR)
MANIVET PHILIPPE (FR)
PIGUET FABIEN (FR)
OULDALI HADJER (FR)
KRUPOVA ZUZANA (FR)
DEFRENAIX PIERRE (FR)
OUKHALED ABDELGHANI (FR)
MANIVET PHILIPPE (FR)
PIGUET FABIEN (FR)
OULDALI HADJER (FR)
KRUPOVA ZUZANA (FR)
DEFRENAIX PIERRE (FR)
Application Number:
PCT/FR2017/000129
Publication Date:
March 08, 2018
Filing Date:
June 26, 2017
Export Citation:
Assignee:
EXCILONE (FR)
International Classes:
G01N15/12; C12Q1/68; G01N27/327; G01N33/487
Other References:
YONG WANG ET AL: "Nanopore Sensing of Botulinum Toxin Type B by Discriminating an Enzymatically Cleaved Peptide from a Synaptic Protein Synaptobrevin 2 Derivative", ACS APPLIED MATERIALS AND INTERFACES, vol. 7, no. 1, 14 January 2015 (2015-01-14), US, pages 184 - 192, XP055362496, ISSN: 1944-8244, DOI: 10.1021/am5056596
JEFF NIVALA ET AL: "Discrimination among Protein Variants Using an Unfoldase-Coupled Nanopore", ACS NANO, vol. 8, no. 12, 23 December 2014 (2014-12-23), US, pages 12365 - 12375, XP055241720, ISSN: 1936-0851, DOI: 10.1021/nn5049987
CHAN CAO ET AL: "Discrimination of oligonucleotides of different lengths with a wild-type aerolysin nanopore", NATURE NANOTECHNOLOGY, vol. 11, no. 8, 25 April 2016 (2016-04-25), GB, pages 713 - 719, XP055362275, ISSN: 1748-3387, DOI: 10.1038/nnano.2016.66
AZIZ FENNOURI ET AL: "Kinetics of Enzymatic Degradation of High Molecular Weight Polysaccharides through a Nanopore: Experiments and Data-Modeling", ANALYTICAL CHEMISTRY, vol. 85, no. 18, 17 September 2013 (2013-09-17), US, pages 8488 - 8492, XP055362279, ISSN: 0003-2700, DOI: 10.1021/ac4020929
JAMES CLARKE ET AL: "Continuous base identification for single-molecule nanopore DNA sequencing", NATURE NANOTECHNOLOGY, vol. 4, no. 4, 1 April 2009 (2009-04-01), pages 265 - 270, XP055028415, ISSN: 1748-3387, DOI: 10.1038/nnano.2009.12
FABIEN PIGUET ET AL: "Electroosmosis through [alpha]-Hemolysin That Depends on Alkali Cation Type", JOURNAL OF PHYSICAL CHEMISTRY LETTERS, vol. 5, no. 24, 18 December 2014 (2014-12-18), US, pages 4362 - 4367, XP055362464, ISSN: 1948-7185, DOI: 10.1021/jz502360c
SÉVERINE BOULON ET AL: "Establishment of a Protein Frequency Library and Its Application in the Reliable Identification of Specific Protein Interaction Partners", MOLECULAR & CELLULAR PROTEOMICS, vol. 9, no. 5, 20 December 2009 (2009-12-20), US, pages 861 - 879, XP055402747, ISSN: 1535-9476, DOI: 10.1074/mcp.M900517-MCP200
ROBERT M MARTIN ET AL: "Principles of protein targeting to the nucleolus", NUCLEUS, vol. 6, no. 4, 4 July 2015 (2015-07-04), pages 314 - 325, XP055405680, ISSN: 1949-1034, DOI: 10.1080/19491034.2015.1079680
ABDELGHANI OUKHALED ET AL: "Sensing Proteins through Nanopores: Fundamental to Applications", ACS CHEMICAL BIOLOGY, vol. 7, no. 12, 21 December 2012 (2012-12-21), US, pages 1935 - 1949, XP055362448, ISSN: 1554-8929, DOI: 10.1021/cb300449t
JEFF NIVALA ET AL: "Discrimination among Protein Variants Using an Unfoldase-Coupled Nanopore", ACS NANO, vol. 8, no. 12, 23 December 2014 (2014-12-23), US, pages 12365 - 12375, XP055241720, ISSN: 1936-0851, DOI: 10.1021/nn5049987
CHAN CAO ET AL: "Discrimination of oligonucleotides of different lengths with a wild-type aerolysin nanopore", NATURE NANOTECHNOLOGY, vol. 11, no. 8, 25 April 2016 (2016-04-25), GB, pages 713 - 719, XP055362275, ISSN: 1748-3387, DOI: 10.1038/nnano.2016.66
AZIZ FENNOURI ET AL: "Kinetics of Enzymatic Degradation of High Molecular Weight Polysaccharides through a Nanopore: Experiments and Data-Modeling", ANALYTICAL CHEMISTRY, vol. 85, no. 18, 17 September 2013 (2013-09-17), US, pages 8488 - 8492, XP055362279, ISSN: 0003-2700, DOI: 10.1021/ac4020929
JAMES CLARKE ET AL: "Continuous base identification for single-molecule nanopore DNA sequencing", NATURE NANOTECHNOLOGY, vol. 4, no. 4, 1 April 2009 (2009-04-01), pages 265 - 270, XP055028415, ISSN: 1748-3387, DOI: 10.1038/nnano.2009.12
FABIEN PIGUET ET AL: "Electroosmosis through [alpha]-Hemolysin That Depends on Alkali Cation Type", JOURNAL OF PHYSICAL CHEMISTRY LETTERS, vol. 5, no. 24, 18 December 2014 (2014-12-18), US, pages 4362 - 4367, XP055362464, ISSN: 1948-7185, DOI: 10.1021/jz502360c
SÉVERINE BOULON ET AL: "Establishment of a Protein Frequency Library and Its Application in the Reliable Identification of Specific Protein Interaction Partners", MOLECULAR & CELLULAR PROTEOMICS, vol. 9, no. 5, 20 December 2009 (2009-12-20), US, pages 861 - 879, XP055402747, ISSN: 1535-9476, DOI: 10.1074/mcp.M900517-MCP200
ROBERT M MARTIN ET AL: "Principles of protein targeting to the nucleolus", NUCLEUS, vol. 6, no. 4, 4 July 2015 (2015-07-04), pages 314 - 325, XP055405680, ISSN: 1949-1034, DOI: 10.1080/19491034.2015.1079680
ABDELGHANI OUKHALED ET AL: "Sensing Proteins through Nanopores: Fundamental to Applications", ACS CHEMICAL BIOLOGY, vol. 7, no. 12, 21 December 2012 (2012-12-21), US, pages 1935 - 1949, XP055362448, ISSN: 1554-8929, DOI: 10.1021/cb300449t
Attorney, Agent or Firm:
CÉLANIE, Christian (FR)
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