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Title:
METHOD FOR THE ELECTROCHEMICAL DETECTION OF TARGET NUCLEIC ACID SEQUENCES
Document Type and Number:
WIPO Patent Application WO2007099236
Kind Code:
B1
Abstract:
The invention relates to a method and a collection for the electrochemical detection of target nucleic acid sequences. According to the method, a biological sample that may contain a nucleic acid is provided, said nucleic acid being capable of containing a target sequence, said biological sample being mixed with an oxidizing agent, said target sequence comprising at least one nucleotide base that can be oxidized by said oxidizing agent; complementary means capable of coupling with said target sequence are provided; according to the invention, said complementary means comprise activatable amplification means suitable for replicating said target sequence, said amplification means comprising at least nucleotides which include said nucleotide base, wherein said nucleotides are able to be consumed during replication so as to constitute replicated nucleic acids; and the presence of said target sequence is determined by applying an electric field to said sample and recording the decrease in the electric current.

Inventors:
MARCHAL DAMIEN (FR)
LIMOGES BENOIT (FR)
DEQUAIRE MURIELLE (FR)
Application Number:
FR2007000373W
Publication Date:
December 13, 2007
Filing Date:
March 02, 2007
Export Citation:
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Assignee:
UNIVERSITE DE PARIS - DENIS DIDEROT
UNIVERSITE DE BOURGOGNE
MARCHAL, DAMIEN
LIMOGES, BENOIT
DEQUAIRE, MURIELLE
International Classes:
C12Q1/68
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