METHOD FOR ELIMINATING STEM CELLS, METHOD FOR PROTECTING DIFFERENTIATED CELLS, AND CULTURE MEDIUM COMPOSITION

Title:

METHOD FOR ELIMINATING STEM CELLS, METHOD FOR PROTECTING DIFFERENTIATED CELLS, AND CULTURE MEDIUM COMPOSITION

Document Type and Number:

WIPO Patent Application WO/2016/163374

Kind Code:

A1

Abstract:

Provided is a method for eliminating stem cells whereby undifferentiated stem cells can be surely eliminated. For this purpose, a cell population, which contains stem cells and somatic cells differentiated and induced therefrom, is cultured in a culture medium composition containing a photosensitizer. Then, the cell population is irradiated with light of a definite wavelength so as to selectively eliminate the stem cells. The stem cells are pluripotent stem cells or somatic stem cells. The pluripotent stem cells comprise either ES cells (embryonic stem cells) or iPS cells (induced pluripotent stem cells). The somatic stem cells comprise any of reproductive stem cells, reproductive cells, stem cells having multiple differentiation potency and stem cells having single differentiation potency.

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Inventors:

YOSHIDA SHUNSUKE (JP)
INAMURA MITSURU (JP)
TANAKA TOHRU (JP)
ISHIKAWA HIROYUKI (JP)
ITO HIDENORI (JP)

Application Number:

PCT/JP2016/061189

Publication Date:

October 13, 2016

Filing Date:

April 06, 2016

Export Citation:

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Assignee:

REPROCELL INCORPORATED (JP)
SBI PHARMACEUTICALS CO LTD (JP)

International Classes:

C12N1/00; C12N5/10; C12N1/02; C12N5/0735

Domestic Patent References:

WO2014157257A1	2014-10-02
WO2014126146A1	2014-08-21
WO2013005379A1	2013-01-10

Other References:

ZHANG BAOQIN ET AL.: "Toxicity to the normal hemocytes by ALA-PDT for the ex vivo purging of hematopoietic stem cell grafts", ACADEMIC JOURNAL OF XI'AN JIAOTONG UNIVERSITY, vol. 20, no. 4, 2008, pages 250 - 255
SUSANTO, JIMMY ET AL.: "Porphyrin ZHomeostasis Maintained by ABCG2 Regulates Self-Renewal of Embryonic Stem Cells", PLOS ONE, vol. 3, no. 12, 2008, pages 5, XP055320025
See also references of EP 3282011A4