The term"exogenous chemical substance"as used herein means a chemical substance, whether naturally or synthetically obtained, which is applied to a plant to result in expressing a desired biological activity. The term"biological activity"as used herein means elicitation of a stimulatory, inhibitory, regulatory, therapeutic, toxic or lethal response in the plant or in a pathogen, parasite or feeding organism present in or on the plant. Examples of exogenous chemical substances include, but are not limited to, chemical pesticides (such as herbicides, algicides, fungicides, bactericides, viricides, insecticides, miticides, nematicides and molluscicides), plant growth regulators, fertilizers and nutrients, gametocides, defoliants, desiccants, mixtures thereof and the like.

The term"biological effectiveness"is used herein to denote the degree to which a desired biological activity is expressed upon application of an exogenous chemical substance to foliage of a plant, or alternatively to denote the dosage or rate of application of the exogenous chemical substance that results in the desired biological activity being expressed to a given degree. For example, where the exogenous chemical substance is a herbicide, biological effectiveness can be measured by the degree of inhibition of plant growth resulting from application of a particular rate of the herbicide, or by the application rate of the herbicide required to cause a particular degree of inhibition, e. g., 50% or 85% inhibition. Thus increased or enhanced biological effectiveness of a herbicide can be exhibited for example as an increased level of plant growth inhibition at a given rate of the herbicide, or as a reduction in the minimum rate of the herbicide giving a certain threshold level of plant growth inhibition.

BACKGROUND OF THE INVENTION For many purposes in agriculture and related endeavors it is desired to treat plants with exogenous chemical substances of various kinds. Many exogenous chemical substances are applied to foliage (i. e., leaves and other non-woody above-ground parts) of a plant, and have a

site of action in the plant either close to or remote from the locus of application. Such substances are referred to herein as foliar-applied exogenous chemical substances.

Typically, when an exogenous chemical substance is applied to foliage by plant treatment processes known in the art, only a small portion of the amount applied reaches sites of action in the plant where a desired biological activity of the exogenous chemical substance can be usefully expressed. It is therefore a major desideratum in agriculture and related endeavors to enhance the efficiency of delivery of foliar-applied exogenous chemical substances to their sites of action in plants, and thereby to enhance the biological effectiveness of the exogenous chemical substance for the purpose for which the exogenous chemical substance is used.

Application to foliage of an exogenous chemical substance by processes known in the art does not universally result in inefficient delivery to sites of action. In some situations such processes provide excellent biological effectiveness, even at a low use rate of the exogenous chemical substance. In other situations the same processes, using the same rate of the same exogenous chemical substance, provide inadequate biological effectiveness. Thus, these processes are inconsistent in the result they provide, or they cannot be relied upon to provide the desired result.

A problem is that it is seldom possible to identify in advance those situations where good biological effectiveness will be obtained, partly because so many factors influence efficiency of delivery. These factors include weather (temperature, relative humidity, daylength, cloudiness, precipitation, wind, etc.) preceding, during and following application, soil conditions (fertility, aeration, etc.), plant growth stage, health and physiological status, equipment-related inaccuracies in application, and other factors. Therefore, to help ensure reliable or consistent biological effectiveness of a foliar-applied exogenous chemical substance, the user typically applies the substance at a higher rate than is truly necessary in the majority of situations.

Variability in biological effectiveness in field conditions is an especially troublesome problem in the case of exogenous chemical substances that are acids, and are typically formulated as water-soluble salts in which the exogenous chemical substance is present in an anionic form. Sometimes by converting such acid substances to esters, this variability can be moderated; however, in many cases esters show reduced biological effectiveness, for example due to inadequate conversion back to the parent acid once inside the treated plant. There remains a strong need for enhanced biological effectiveness, and enhanced reliability of biological

effectiveness, of foliar-applied exogenous chemical substances, particularly anionic exogenous chemical substances.

The term"anionic exogenous chemical substance"as used herein means an exogenous chemical substance whose molecular structure includes one or more acid, or proton-donating, sites, and is therefore capable of forming an anion in the presence of a proton acceptor. The term therefore embraces substances that are zwitterionic. In describing an exogenous chemical substance as"anionic"herein, it is not implied that the exogenous chemical substance is necessarily in anionic form or that it is dissociated.

Benefits of a process providing greater reliability of biological effectiveness include an ability to reduce rates of application of exogenous chemical substances without sacrificing consistency of biological effectiveness. Pressures felt by the agricultural industry to reduce pesticide, particularly herbicide, usage are well evidenced by symposia on the subject, such as that held in 1993 by the Weed Science Society of America and documented in Weed Technology 8,331-386 (1994). Reduced use rates bring rewards not only environmentally but also economically, as the cost per unit area treated decreases.

Foliar-applied exogenous chemical substances have frequently been applied together with amphiphilic materials, particularly amphiphilic surface-active agents, otherwise known as surfactants. Surfactants can influence biological effectiveness of a foliar-applied exogenous chemical substance in numerous ways.

When a dilute aqueous composition of an exogenous chemical substance is applied to foliage by conventional hydraulic spraying, the presence of surfactant in the dilute aqueous composition can alter the size distribution of the spray droplets, typically increasing the percentage of spray volume in the form of small droplets and reducing the percentage of spray volume in the form of large droplets. As smaller droplets have lower momentum than larger droplets, these smaller droplets are less likely to rebound from a foliar surface and consequently are more likely to be retained on that surface. Spray retention can also be facilitated by adhesion of surfactant molecules in a spray droplet to the foliar surface, which in most plants is waxy and hydrophobic. This adhesion reduces not only rebound but also run-off of spray droplets from the foliar surface. Surfactants also tend to increase the area of contact between a spray droplet and a foliar surface, and in many cases enhance penetration of an exogenous chemical substance from the droplet into and through cuticles of leaves to reach internal leaf tissues.

Through these and perhaps other effects, amphiphilic materials including surfactants have long been known to increase the biological effectiveness of exogenous chemical substances. It is therefore commonplace for one or more surfactants to be included in commercial formulations of foliar-applied exogenous chemical substances, even in formulations that do not require the presence of surfactants for acceptable physical stability or handling properties, for example as emulsifying or suspending agents or dispersants.

One of the most extensively studied of foliar-applied anionic exogenous chemical substances, from the point of view of the role of surfactants in enhancing biological effectiveness, is the herbicide glyphosate. As well as being a phytotoxic agent, glyphosate has been used as a plant growth regulator.

Glyphosate (N-phosphonomethylglycine) in its strict sense is an acid compound, but the word"glyphosate"is herein used in a less restrictive sense, except where the context dictates otherwise, to encompass not only glyphosate acid but also salts, adducts and esters thereof, and compounds which are converted to glyphosate in plant tissues or which otherwise provide glyphosate ions. In most commercial formulations of glyphosate, the glyphosate is present as a water-soluble salt. In this respect, glyphosate is typical of most exogenous chemical substances that are acids or that form anions.

Herbicidal salts of glyphosate are disclosed, for example, in U. S. Patent No. 3,799,758 to Franz, U. S. Patent No. 3,853,530 to Franz, U. S. Patent No. 4,140,513 to Prill, U. S. Patent No.

4,315,765 to Large, U. S. Patent No. 4,405,531 to Franz, U. S. Patent No. 4,481,026 to Prisbylla and U. S. Patent No. 4,507,250 to Bakel. Typical of such salts are alkali metal, for example sodium and potassium, salts; ammonium salt; and numerous salts having an ammonium, sulfonium or sulfoxonium cation substituted with 1-3 organic groups containing in total 1-6 carbon atoms, for example dimethylammonium, isopropylammonium, ethanolammonium and trimethylsulfonium salts.

Commercial formulations of glyphosate salts include, for example, Roundups, Accords, Roundup (R) Ultra and Roundup Xtra herbicides of Monsanto Company, which contain the isopropylammonium salt, Roundup'2) Dry and Rivals herbicides of Monsanto Company, which contain the ammonium salt, Roundup Geoforce herbicide of Monsanto Company, which contains the sodium salt, and Touchdown herbicide of Zeneca, which contains the trimethylsulfonium salt.

Glyphosate as a herbicide has many advantages, particularly environmental advantages including biodegradability and low ecotoxicity. However, studies have shown that even the most biologically effective formulations of glyphosate presently in use do not deliver glyphosate efficiently to sites in the plant where the glyphosate exerts its phytotoxic effect. Typically, only a small fraction of the applied herbicide arrives at such sites.

The small fraction of applied glyphosate which reaches sites of phytotoxic action is related to the fact that the glyphosate must go through several barriers. Among these, one of the most important is believed to be the lipophilic cuticle on the foliar surface to which the glyphosate is applied. It has therefore been theorized that it would be desirable to place the glyphosate into an amphiphilic medium which would provide greater compatibility between the lipophilic cuticle and the hydrophilic glyphosate, and thereby facilitate penetration of glyphosate into and through the cuticle. Similar thinking has been applied to other exogenous chemical substances, particularly those typically formulated as water-soluble salts.

That the concept of an amphiphilic medium as an aid to cuticular penetration and thereby enhanced biological effectiveness, for example of glyphosate, has validity is demonstrated by many studies in which foliar uptake or effectiveness has been enhanced by surfactants. An extensive study by Wyrill & Burnside, Weed Science 25,275-287,1977 led to a conclusion that "an effective surfactant is a critical component of any glyphosate spray mixture", but noted great variation among surfactant types in the degree of enhancement of herbicidal effectiveness afforded. The authors cautioned that"effectiveness of surfactant combinations was quite variable and difficult to predict"and that"the indiscriminate addition of surfactants or wetting agents to glyphosate spray mixtures which already contain a surfactant should be avoided". The Wyrill & Burnside study generally involved application of surfactants at very high rates relative to the rates of glyphosate applied. Under these conditions, and on the particular plant species they studied, the authors found that in general, cationic surfactants gave greater enhancement of the herbicidal effectiveness of glyphosate than did nonionic surfactants. It is well accepted by those of skill in the art that relative effectiveness of surfactants in enhancing biological activity of an exogenous chemical substance, particularly glyphosate, at high surfactant levels does not permit reliable prediction of relative effectiveness at much lower surfactant levels, as in the realm of the present invention.

Data are reported in International Publication No. WO 98/06259 for a wide variety of cationic, nonionic, anionic and amphoteric surfactants applied either in mixture with, or in sequence following, a glyphosate composition.

The term"alkyl", as used herein in relation to hydrophobic moieties of surfactants, has the broad meaning, as conventionally applied in surfactant art, of aliphatic hydrocarbyl, embracing unsaturated groups, for example alkenyl and alkynyl groups, as well as saturated alkyl groups, unless the context demands otherwise.

Surfactants having a hydrophilic moiety comprising one or more protonatable amino groups or cationic ammonium groups together with a total of 1 to about 100 oxyethylene units in one or more oxyethylene chains constitute a favored selection of surfactants useful in formulating glyphosate and other anionic exogenous chemical substances. For example, commercial glyphosate herbicide products marketed under the trademark Roundup have been formulated with surfactant compositions based on polyoxyethylene C822 alkylamines. For example, the surfactant composition MON 0818 of Monsanto Company, which has been extensively used in the formulation of Roundup (S) herbicide, contains a polyoxyethylene tallowamine having an average of about 15 oxyethylene units per molecule.

Numerous patents disclose compositions comprising glyphosate and an oxyethylene or polyoxyethylene amine or ammonium surfactant.

U. S. Patent No. 5,668,085 to Forbes et al. discloses compositions comprising glyphosate and a polyoxyethylene C8-22 alkylamine surfactant having an average of up to about 12 oxyethylene units per molecule. Australian Patent Application No. 57565/90 discloses compositions comprising glyphosate and a polyoxyethylene C8-22 alkyldiaminopropane surfactant. U. S. Patent No. 5,317,003 to Kassebaum & Berk discloses compositions comprising glyphosate and a quaternary polyoxyethylene C6 l4 alkylmethylammonium surfactant having about 5 to about 50 oxyethylene units per molecule. U. S. patent No. 5,652,197 to Claude et al. discloses compositions comprising glyphosate and a quaternary polyoxypropylene oxyethylene tri- (CI-3 alkyl) ammonium surfactant having 2 to 20 oxypropylene units per molecule. U. S.

Patent No. 5,118,444 to Nguyen discloses cempositions comprising glyphosate and a polyoxyethylene C6-20 alkylamine oxide surfactant having about 5 to about 25 oxyethylene units per molecule. U. S. Patent No. 5,750,468 to Wright discloses compositions comprising glyphosate and a polyoxyethylene tertiary alkyletheramine, polyoxyethylene quaternary

alkyletherammonium or polyoxyethylene alkyletheramine oxide surfactant. French Patent Application No. 2 648 316 discloses compositions comprising glyphosate and a polyoxyethylene N-alkyl-1,3-diaminopropane surfactant.

Polyoxyethylene Cl622 alkylether surfactants have been less frequently disclosed in compositions with glyphosate, and generally at surfactant to glyphosate weight ratios outside the realm of the present invention. For example, European Patent No. 0 206 537 discloses solid compositions comprising glyphosate and Plurafac A-39 surfactant of BASF, which is a polyoxyethylene C16-18 alkylether surfactant having an average of about 55 oxyethylene units per molecule. The lowest weight ratio of Plurafac A-39 to glyphosate acid equivalent disclosed therein can be calculated as about 1.16: 1 (composition 12 of Table IV of the cited patent).

Wyrill & Burnside, op. cit., disclose plant treatment compositions comprising glyphosate and a 1: 1 mixture of a polyoxyethylene alkylether surfactant identified as Plurafac A-46 with polyoxyethylene alkylamine surfactants Ethomeen T/15 of Akzo and MON 0818 of Monsanto, at surfactant to glyphosate ratios far outside the realm of the present invention. Probably because the surfactant concentration in the compositions was so high (1% weight/volume), no significant benefit was evident for the mixtures over the alkylamine surfactants alone.

At lower surfactant to glyphosate weight ratios, strong enhancement of glyphosate herbicidal effectiveness has been reported for mixtures of a polyoxyethylene alkylamine or alkylammonium surfactant and a polyoxyethylene alkylether surfactant, where the alkylether is derived from a secondary alcohol, as in International Publication No. WO 95/16351, a Guerbet alcohol, as in U. S. Patent No. 5,663,117 to Warner, or an acetylenic diol, as in U. S. Patent No.

5,639,711 to Kassebaum et al. European Patent Application No. 0 582 561 discloses a solid granular glyphosate composition containing a polyoxyethylene quaternary alkylammonium surfactant (EthoquadTM 18/25 of Akzo) and a polyoxyethylene C 13 alkylether surfactant (Trycol 5943 of Henkel) but does not report herbicidal effectiveness of this composition.

Townson, in her Ph. D. thesis, Influence offormulation and application variables in relation to the performance of glyphosate and imazapyr for control of Imperata cylindrica (L.) Raeuschel, University of Bristol, U. K., 312 pp., 1990, compared polyoxyethylene C16-18 alkylethers having respectively 3,12 and 19 oxyethylene units for enhancement of foliar retention, uptake, translocation and herbicidal effectiveness of glyphosate and imazapyr.

Comparison was also made with polyoxyethylene alkylethers having shorter alkyl chain lengths

(C9 Ci2-i5 and ¬13-15). The study further included polyoxyethylene alkylamine surfactants but no blends of alkylether and amine surfactants were tested.

It is an objective of the present invention to provide a new method of enhancing the biological effectiveness of an exogenous chemical substance, in particular an anionic exogenous chemical substance, applied to foliage of a plant.

Another object of the invention is to provide a useful alternative to existing methods for enhancing biological effectiveness of anionic exogenous chemical substances.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a graphical plot of the natural logarithm of amplitude of glyphosate proton NMR resonance,"ln (amplitude)", against the square of field gradient,"gradient2", for composition 24-04 of the present invention, as explained in Example 24. The plotted data form a curve that can be resolved into two straight-line components, one representing a free pool of glyphosate and one representing an entrapped pool of glyphosate.

Figure 2 is a graphical plot of the fraction of glyphosate in the entrapped pool against the weight ratio of lecithin to MON 0818, as explained in Example 24.

SUMMARY OF THE INVENTION In one embodiment of the present invention, a method is provided for eliciting biological activity of an anionic exogenous chemical substance applied to foliage of a plant, comprising (i) preparing a plant treatment composition that comprises (a) water; having dissolved or dispersed therein (b) an anionic exogenous chemical substance in a biologically effective amount; (c) an alkylether surfactant component consisting of one or more surfactants each having the formula (I) R"-0- (CH2CH20). ( (CHR) 20)R" (I) wherein R12 is an aliphatic saturated or unsaturated hydrocarbyl group having about 16 to about 22 carbon atoms, n is an average number of about 5 to about 100, m is an average number of 0 to about 5, one R in each- ( (CHR) 20)- group is hydrogen and the other R is methyl, and Rl3 is a hydrogen, Cl 4 alkyl or C24 acyl group; and

(d) an amine surfactant component consisting of one or more surfactants each having a molecular structure that comprises (1) a hydrophobic moiety having one or a plurality of independently saturated or unsaturated, branched or unbranched, aliphatic, alicyclic or aromatic C3 20 hydrocarbyl or hydrocarbylene groups joined together by 0 to about 7 ether linkages and having in total about 8 to about 24 carbon atoms, and (2) a hydrophilic moiety comprising an amino group that is cationic or that can be protonated to become cationic, having attached directly thereto 1 to 3 oxyethylene groups or polyoxyethylene chains, these oxyethylene groups and polyoxyethylene chains comprising on average 1 to about 50 oxyethylene units per surfactant molecule, the hydrophobic moiety being attached either to the amino group or via an ether linkage to an oxyethylene unit; the weight ratio of the alkylether surfactant component to the amine surfactant component being about 1: 10 to about 10: 1; wherein the alkylether and amine surfactant components are present in total in an adjuvant amount of about 0.05 to about 0.5 parts by weight per part by weight of the anionic exogenous chemical substance, expressed as acid equivalent; and (ii) applying a biologically effective amount of the plant treatment composition to foliage of the plant; whereby the alkylether and amine surfactant components interact synergistically to elicit greater biological effectiveness of the anionic exogenous chemical substance than would be elicited by either component alone at the same total surfactant concentration in the plant treatment composition.

In another embodiment of the present invention, a method is provided for eliciting biological activity of an anionic exogenous chemical substance applied to foliage of a plant, comprising (i) preparing a plant treatment composition as described immediately above; and

(ii) applying a biologically effective amount of the plant treatment composition to foliage of the plant within about 6 hours prior to rain or activation of overhead irrigation on the plant.

In this embodiment, rainfastness of the composition has in many instances been found to be surprisingly good.

In yet another embodiment of the present invention, a method is provided for eliciting herbicidal activity of an anionic herbicide applied to foliage of a plant, comprising (i) preparing a plant treatment composition that comprises (a) water; having dissolved or dispersed therein (b) a first herbicide that is anionic and is present in a herbicidally effective amount; (c) a second herbicide that is present in an amount effective to elicit a visible symptom of phytotoxicity in the plant within about 4 days after application to the foliage; (d) an alkylether surfactant component as described immediately above; and (e) an amine surfactant component as described immediately above; the weight ratio of the alkylether surfactant component to the amine surfactant component being about 1: 10 to about 10: 1; wherein the alkylether and amine surfactant components are present in total in an adjuvant amount of about 0.05 to about 0.5 parts by weight per part by weight of the first herbicide, expressed as acid equivalent; and (ii) applying a biologically effective amount of the plant treatment composition to foliage of the plant.

In this embodiment, a surprisingly low level of antagonism, or even total absence of antagonism, of the herbicidal effectiveness of the first herbicide by the presence of the second herbicide has been observed. The plant treatment composition used in this embodiment is itself an embodiment of the present invention.

Amine surfactants useful in the present invention preferably have a chemical structure that, when present in an aqueous medium having a pH of about 4, can be individually represented by the formula (II)

where Re is hydrogen or a linear or branched C8-20 aliphatic hydrocarbyl group; each z is independently 2 or 3; each Rd is hydrogen or methyl whereby if z is 2 at least one Rd in the two- (CHRd} groups is methyl; y is 0 to 7 such that the total number of carbon atoms in the group Rc-(O-(CHRd)z)y- is 8 to 24; x is 0 to 5; Rf is hydrogen, C14 alkyl or benzyl; Rg is C14 alkyl or- (CHZCHZ-O) x. R' and Rh is C1-4 alkyl or -(CH2CH2-O) XRC,(CH2CH2-O) XRC, where Rc is hydrogen, C14 alkyl or C2-4 acyl and x'and x"are average numbers such that x + x'+ x" (the total number of oxyethylene units in a molecule of the amine surfactant) is 1 to about 50; Zizis a suitable anion; and p is 1 or 2; or by the formula (III) where Rd,Rc,Rg,Rh, x, y and z are as defined immediately above and Y- is an anionic group selected from -O-, -(CHRb)w-COO- and -(CHRb)w-SO3- where w is 1 to 3 and each Rb is independently hydrogen, hydroxyl, C alkyl or hydroxy- (C 1-4 alkyl).

Other useful amine surfactants include those that can be represented by the formula (IV) where Rd, Re, x, y and z are as defined immediately above, v is a number from 2 to 6, and R', Rt and R"are independently C1-4 alkyl or -(CH2CH2-O)kRc where Rc is hydrogen, C 1-4 alkyl or C1-4 acyl and each k is an average number such that the total number of oxyethylene units in a molecule of the amine surfactant is 1 to about 50.

The alkylether surfactant (s) and the amine surfactant (s), when provided together as a surfactant mixture in a ratio in the indicated range, have been found generally to have at least one of the following unexpected effects:

(i) the surfactants interact in a synergistic manner in enhancing biological effectiveness of the anionic exogenous chemical substance when the plant treatment composition is applied to foliage of a plant; (ii) the surfactants together provide surprisingly enhanced rainfastness when the plant treatment composition is applied to foliage of a plant and rain falls or overhead irrigation is activated shortly (e. g., within about 6 hours) after application; (iii) the surfactants together unexpectedly reduce antagonism of biological effectiveness of the anionic exogenous chemical substance by a second exogenous chemical substance also present in the composition, in particular where the exogenous chemical substances are herbicides.

A"synergistic"interaction between one or more alkylether surfactants and one or more amine surfactants is to be understood herein as one satisfying the following test, notwithstanding any other definition of synergism that has been applied in the art. This test is conducted using a total amount of alkylether and/or amine surfactants that is a"suboptimal adjuvant amount"as defined herein. If such total amount of a mixture of alkylether and amine surfactants elicits biological effectiveness of a co-applied anionic exogenous chemical substance that is greater than the biological effectiveness elicited either by the same total amount of the alkylether surfactant (s) in the substantial absence of amine surfactant, or by the same total amount of the amine surfactant (s) in the substantial absence of alkylether surfactant, the alkylether and amine surfactant components of the mixture are considered to interact in a synergistic manner.

An"adjuvant amount"of a surfactant or mixture of surfactants is an amount sufficient, when applied to plant foliage together with an anionic exogenous chemical substance, to elicit a degree of biological effectiveness that is observably greater than that provided by the anionic exogenous chemical substance applied in the absence of any surfactant. A"suboptimal adjuvant amount"of a surfactant or mixture of surfactants is an adjuvant amount eliciting a degree of biological effectiveness that is observably less than that elicited by the same surfactant or mixture of surfactants in twice the amount. Surfactant amounts are conveniently expressed as weight/volume concentrations in aqueous plant treatment compositions and as weight/weight concentrations in liquid or solid concentrate compositions.

The present invention is not to be construed as being limited to situations where the surfactant mixture is used in a suboptimal adjuvant amount. Only the test for synergism between

the alkylether and amine components of the surfactant mixture requires application in a suboptimal adjuvant amount.

It is further to be understood that the synergistic interaction between the alkylether and amine surfactants characteristic of compositions of the invention is not necessarily exhibited on all plant species or under all application conditions, being subject to the normal variability of complex biological phenomena. However, the generally superior biological effectiveness resulting from this synergistic interaction is exhibited with sufficient frequency and consistency to represent a major advance in the art.

By"rainfastness"herein is meant the degree to which biological effectiveness of an exogenous chemical substance is maintained when rain falls or overhead irrigation is activated shortly after foliar application of a plant treatment composition containing the exogenous chemical substance. Rainfastness can be measured by comparing biological effectiveness with and without rain or overhead irrigation. A suitable test for rainfastness involves overhead watering of treated plants by means of a sprinkler or spray system, in an amount of about 2.5 to about 25 mm at a rate of about 10 to about 100 mm/hour, beginning about 5 minutes to about 6 hours after application of the plant treatment composition; and recording biological effectiveness by comparison with treated plants not subjected to such overhead watering.

By"antagonism"herein is meant a reduction in biological effectiveness of a first exogenous chemical substance when applied in a plant treatment composition, such reduction resulting from the inclusion in the same plant treatment composition of a second exogenous chemical substance.

A plant treatment composition useful in the method of the invention can be provided to the end-user by a commercial manufacturer or formulator as a"ready-to-use"product. As an alternative, the plant treatment composition can be prepared by the end-user by dissolving, dispersing or diluting in water a first concentrate composition containing the anionic exogenous chemical substance, a second concentrate composition containing the alkylether component of the surfactant mixture and a third concentrate composition containing the amine component of the surfactant mixture. As a further alternative, the plant treatment composition can be prepared by the end-user by dissolving, dispersing or diluting in water a first concentrate composition containing the anionic exogenous chemical substance and a second concentrate composition containing the surfactant mixture. As a still further alternative, the plant treatment composition

can be prepared by the end-user by dissolving, dispersing or diluting in water a single concentrate composition containing the anionic exogenous chemical substance and the surfactant mixture. Other ways of preparing the plant treatment composition will be apparent to those of skill in the art.

Also useful in practice of the present invention is a concentrate composition for application in an aqueous carrier to foliage to elicit a biological effect, comprising about 10% to about 90% by weight of an anionic exogenous chemical substance expressed as acid equivalent (a. e.), together with an alkylether surfactant and an amine surfactant such that, when the concentrate composition is dissolved, dispersed or diluted in a suitable volume of water, a plant treatment composition useful in the method of the invention as provided above is formed. Such concentrate composition can be solid or liquid. A contemplated solid concentrate composition contains up to about 90% a. e. by weight of the exogenous chemical substance. A contemplated liquid concentrate composition contains up to about 50% by weight of the exogenous chemical substance expressed as acid equivalent (a. e.).

DETAILED DESCRIPTION OF THE INVENTION The anionic exogenous chemical substance Examples of anionic exogenous chemical substances that can be used in compositions useful in the method of the present invention include, but are not limited to, chemical pesticides (such as herbicides, algicides, fungicides, bactericides, viricides, insecticides, aphicides, miticides, nematicides and molluscicides), plant growth regulators, fertilizers and nutrients, gametocides, defoliants, desiccants, mixtures thereof and the like. Although the disclosure herein relates to"an anionic exogenous chemical substance", it is to be understood that more than one anionic exogenous chemical substance can be included if desired.

A preferred group of anionic exogenous chemical substances consists of those that are normally applied post-emergence to foliage of plants, i. e., foliar-applied anionic exogenous chemical substances. An especially preferred group of foliar-applied anionic exogenous chemical substances consists of those that are systemic in plants, that is, translocated to some extent from their point of entry in the foliage to other parts of the plant where they can usefully exert their desired biological effect.

Especially preferred among these are herbicides, plant growth regulators and nematicides, particularly those that have a molecular weight, excluding counterions, of less than about 300.

Among such compounds, an even more preferred category consists of nematicides such as those disclosed in U. S. Patent No. 5,389,680, the disclosure of which is incorporated herein by reference. Preferred nematicides of this group are 3,4,4-trifluoro-3-butenoic acid or N- (3,4,4- trifluoro-1-oxo-3-butenyl)glycine.

In one embodiment, the anionic exogenous chemical substance is a herbicide. Suitable herbicides include, without restriction, acifluorfen, asulam, benazolin, bentazon, bilanafos, bromacil, bromoxynil, carfentrazone, chloramben, clopyralid, 2,4-D, 2,4-DB, dalapon, dicamba, dichlorprop, diclofop, endothall, fenac, fenoxaprop, flamprop, fluazifop, flumiclorac, fluoroglycofen, fomesafen, fosamine, glufosinate, glyphosate, haloxyfop, imazameth, imazamethabenz, imazamox, imazapic, imazapyr, imazaquin, imazethapyr, ioxynil, MCPA, MCPB, mecoprop, methylarsonic acid, naptalam, nonanoic acid, picloram, quinclorac, quizalofop, sulfamic acid, 2,3,6-TBA, TCA, triclopyr and agriculturally acceptable salts thereof.

Especially preferred herbicides are those whose molecular structure comprises at least one of each of amine, carboxylate, and either phosphonate or phosphinate functional groups. This category includes the herbicides N-phosphonomethylglycine (glyphosate) and DL-homoalanin- 4-yl (methyl) phosphinate (glufosinate). Another preferred group of herbicides are those of the imidazolinone class, including imazameth, imazamethabenz, imazamox, imazapic, imazapyr, imazaquin and imazethapyr.

The invention is illustrated herein by particular reference to glyphosate. Although glyphosate has three acid sites, and can therefore form tribasic salts, preferred aqueous compositions have a pH value not greater than about 8, at which pH value the fraction of glyphosate existing as a tribasic salt is negligibly small. Only the two acid sites that are significantly deprotonated at pH 8 are therefore considered herein. One of these is on the phosphonate moiety, and the other is on the carboxylate moiety, of the glyphosate molecule. At a pH of around 4 to 5, monovalent glyphosate anions predominate.

In plant treatment compositions useful in the method of the invention, the amount of anionic exogenous chemical substance present, in all forms thereof, is sufficient when applied to foliage of a plant to elicit the desired biological activity. Such compositions are sometimes referred to as"spray compositions","sprayable compositions"or"ready-to-use compositions" and typically contain about 0.02% by weight to about 2% by weight of the anionic exogenous

chemical substance, expressed as acid equivalent (a. e.). For some purposes such compositions can contain up to about 5% a. e. by weight or even 10% a. e. by weight.

In concentrate compositions useful in practicing the invention, the amount of anionic exogenous chemical substance present, in all forms thereof, is sufficient, upon dilution, dissolution or dispersion in a suitable volume of water to form a plant treatment composition, and upon application of the plant treatment composition to foliage of a plant, to elicit the desired biological activity.

As a significant portion of the cost of a packaged concentrate composition is the volume- related cost of packaging, transport and storage, it is desirable to increase to the maximum practicable extent the concentration, or loading, of exogenous chemical substance in the composition. Generally the factor that limits loading of a liquid composition is physical stability of the composition under a range of storage conditions. The upper limit of loading, particularly in a liquid concentrate composition, depends on the nature and concentration of other ingredients in the composition and can be readily determined by routine experimentation using procedures known in the art.

Although the anionic exogenous chemical substance can be present in its acid form, it is preferred that it be present predominantly in the form of a salt or mixture of salts. Preferably each such salt is water-soluble and has a cationic counterion of molecular weight lower than about 100. In especially preferred water-soluble salts the cationic counterion is monovalent and is selected from alkali metal cations, ammonium cations, and organic ammonium and sulfonium cations having in total 1-6 carbon atoms.

In particular where the anionic exogenous chemical substance is glyphosate, illustrative cationic counterions suitable for use in compositions of the invention are sodium, potassium, ammonium, dimethylammonium, isopropylammonium, monoethanolammonium and trimethylsulfonium cations.

Throughout this specification, all references to an anionic exogenous chemical substance in general can be taken to apply to glyphosate in particular, unless the context demands otherwise.

The alkylether surfactant As indicated above, the alkylether surfactant component of compositions useful in the method of the invention comprises one or more surfactants each having the formula (I)

R"-O- (CH2CH20) n ( (CHR) 20) n,-R" (I) wherein Rl2 is an aliphatic saturated or unsaturated hydrocarbyl group having about 16 to about 22 carbon atoms, n is an average number of about 5 to about 100, m is an average number of 0 to about 5, one R in each- ((CHR) 2O)-group is hydrogen and the other R is methyl, and Rl3 is a hydrogen, C14 alkyl or C24 acyl group.

Preferably RIZ is linear, more preferably a linear Cl6 or C 18 alkyl, alkenyl or alkadienyl group, for example a cetyl, stearyl, oleyl or linoleyl group. Advantageously the alkylether surfactant component is a mixture of surfactants having various R12 groups. For example, it may be a product described in cosmetic literature as"ceteareth", wherein R12 groups are predominantly cetyl and stearyl groups. Alternatively, the alkylether surfactant component can be derived from a natural oil or fat. For example, if the source is beef tallow, R12 groups are predominantly cetyl, stearyl and oleyl. If the source is corn oil or soybean oil, Rl2 groups are predominantly oleyl and linoleyl. If the source is palm oil, Rl2 groups are predominantly cetyl and oleyl. If the source is cottonseed oil, R groups are predominantly cetyl, oleyl and linoleyl.

Preferably n (the average number of oxyethylene units) is about 7 to about 50, more preferably about 10 to about 40. Preferably m is 0 and Rl3 is hydrogen.

Among particularly preferred alkylether surfactants are ceteth-10, available for example as Brij 56 of ICI; ceteth-20, available for example as Brij 58 of ICI; steareth-20, available for example as BrijTM 78 of ICI, Emthox 5888-A of Henkel and STA-20 of Heterene; steareth- 30, available for example as STA-30 of Heterene; ceteareth-20, available for example as HetoxolTM CS-20 of Heterene; ceteareth-27, available for example as Plurafac A-38 of BASF; and oleth-20, available for example as BrijTM 98 of ICI and Trycol 5971 of Henkel.

The amine surfactant As indicated above, the amine surfactant component of compositions useful in the method of the invention preferably comprises one or more surfactants each having, at a pH of about 4, the formula (II) or the formula (III)

where Re is hydrogen or a linear or branched C8-20 aliphatic hydrocarbyl group; each z is independently 2 or 3; each Rd is hydrogen or methyl whereby if z is 2 at least one Rd in the two- (CHRd)- groups is methyl; y is 0 to 7 such that the total number of carbon atoms in the group Rc-(O-(CHRd)z)y- is 8 to 24; x is 0 to 5; Rf is hydrogen, CI-4 alkyl or benzyl; Rg is Cl 4 alkyl or- (CH2CH2-O) XRC and Rh is C1-4 alkyl or -(CH2CH2-O) XRC,(CH2CH2-O) XRC, where Rc is hydrogen, C1-4 alkyl or C2-4 acyl and x'and x"are average numbers such that x + x'+ x" (the total number of oxyethylene units in a molecule of the amine surfactant) is 1 to about 50; ZP-is a suitable anion; p is 1 or 2; and Y~ is an anionic group selected from-0-,- (CHR b),-COO-and- (CHk),-S03- where w is 1 to 3 and each Rb is independently hydrogen, hydroxyl, C1-4 alkyl or hydroxy- (C 1-4 alkyl). Other useful amine surfactants include those that can be represented by the formula (IV) where Rd, Re, x, y and z are as defined immediately above, v is a number from 2 to 6, and Rs, RI and Ru are independently C1-4 alkyl or -(CH2CH2-O)kRc where Rc is hydrogen, C 4 alkyl or C24 acyl and each k is an average number such that the total number of oxyethylene units in a molecule of the amine surfactant is 1 to about 50.

When a maximum or minimum"average number"is recited herein with reference to a structural feature such as oxyethylene units, it will be understood by those skilled in the art that the integer number of such units in individual molecules in a surfactant preparation typically varies over a range that can include integer numbers greater than the maximum or smaller than the minimum"average number". The presence in a composition of individual surfactant molecules having an integer number of such units outside the stated range in"average number" does not remove the composition from the scope of the present invention, so long as the"average number"is within the stated range and other requirements are met.

Illustrative surfactant types that can be useful as part or all of the amine surfactant component of compositions useful in the method of the invention include the following: (A) Surfactants of formulas (II) or (III) where Re is a C820 aliphatic, saturated or unsaturated, linear or branched hydrocarbyl chain and y is 0. In this group Re alone forms the hydrophobic moiety of the surfactant and is attached directly to the amine or ammonium group, as in alkylamines, or by an ether linkage formed by the oxygen atom of an oxyethylene group or the terminal oxygen atom of a polyoxyethylene chain, as in certain alkyletheramines. Illustrative subtypes having different hydrophilic moieties include: (A-1) Surfactants of formula (II) wherein Rg is- (CH2CH2-0),. H and R h is (CH2CH2-O) H where x'+ x"is an average number of 2 to about 30, and Rf is hydrogen or methyl. This subtype includes commercial surfactants known in the art or referred to herein as"polyoxyethylene alkylamines" (where x is 0 and Rf is hydrogen), certain"polyoxyethylene alkyletheramines" (where x is 1-5 and Rf is hydrogen), and "polyoxyethylene N-methyl alkylammonium salts", illustratively chloride salts (where x is 0, Rf is methyl, ZP-is a chloride anion and p is 1). Suitable examples are polyoxyethylene (2) cocoamine, polyoxyethylene (5) tallowamine, polyoxyethylene (10) cocoamine and polyoxyethylene (15) tallowamine, available for example from Akzo as EthomeenTM C/12, Ethomeen T/15, EthomeenTM C/20 and Ethomeen T/25 respectively, a surfactant conforming, when its amine group is non-protonated, to the formula where Re is Cl2 5 alkyl, x is 3 and x'+ x"is an average number of about 5, as disclosed in U. S. Patent No. 5,750,468, and polyoxyethylene (2) N-methyl cocoammonium chloride, polyoxyethylene (2) N-methyl stearylammonium chloride, polyoxyethylene (10) N-methyl tallowammonium chloride and polyoxyethylene (15) N-methyl cocoammonium chloride, available for

example from Akzo as EthoquadTM18/12,EthoquadTMC/12, T/20 and EthoquadTM C/25 respectively. In cases where Rf is hydrogen, i. e., in tertiary amine surfactants as opposed to quaternary ammonium surfactants, the anion ZP-is typically not supplied with the surfactant.

However, in a glyphosate-containing formulation at a pH of about 4-5, it will be recognized that the anion ZP-can be an anion of the anionic exogenous chemical substance, for example glyphosate.

(A-2) Surfactants of formula (II) where x is 1-5, and R 9 R'and R are independently Cl 4 alkyl. This subtype includes certain"polyoxyethylene N-methyl alkyletherammonium salts", illustratively chloride salts (where Rf, R'and Rh are each methyl, Zizis a chloride anion and p is 1).

(A-3) Surfactants of formula (III) wherein Y- is an anionic oxide group. This subtype includes commercial surfactants known in the art or referred to herein as"polyoxyethylene alkylamine oxides" (where x is 0, Rg is- (CH2CH2-O) X H and Rh is dCH2CH2-O) x H where x'+ x"is an average number of 2 to about 30), and certain"polyoxyethylene alkyletheramine oxides" (where x is 1-5, Rg is -(CH2CH2-O)x'H and Rh is ACH2CH2-O) x H where x'+ x"is an average number of 2 to about 30). Suitable examples are polyoxyethylene (2) cocoamine oxide, sold by Akzo as AromoxTM C/12, and polyoxyethylene (10-20) tallowamine oxides, as disclosed in U. S.

Patent No. 5,118,444.

(B) Surfactants of formulas (II) or (III) where Reis a Cl-tu aliphatic, saturated or unsaturated, linear or branched hydrocarbyl chain, y is 1, z is 3, each Rd is hydrogen, and n is 0. In this group Re-O (CH2) 3- forms the hydrophobic moiety of the surfactant which is attached directly to the amine or ammonium group. These surfactants form a category of alkyletheramines as disclosed in U. S. Patent No.

5,750,468. Illustrative subtypes have the different hydrophilic moieties exemplified in (A-1) and (A-3) above. Suitable examples are a surfactant conforming, when its amine group is non-protonated, to the formula a surfactant conforming to the formula and a surfactant conforming to the formula

where, in each of the three formulas immediately above, Re is C12-15 alkyl and x'+ x"is an average number of about 5, as disclosed in U. S. Patent No. 5,750,468.

(C) Surfactants of formula (II) or (III) where Re is a Cs-is aliphatic, saturated or unsaturated, linear or branched hydrocarbyl chain, y is 1-5, each-O-(CHRd) z-is a group-OCH (CH3) CH2- and x is 0. In this group Re together with the -OCH (CH3) CH2- group or groups forms the hydrophobic moiety of the surfactant which is attached directly to the amino function. These surfactants form a further category of alkyletheramines as disclosed in U. S. Patent No. 5,750,468. Illustrative subtypes have the different hydrophilic moieties exemplified in (A-1) and (A-3) above. A suitable example is a surfactant of formula (II) conforming, when its amine group is non-protonated, to the formula where Rt is Cl2 5 alkyl, y is 2 and x'+ x"is an average number of about 5, as disclosed in U. S. Patent No. 5,750,468.

(D) Surfactants of formula (II) where Re is hydrogen, y is 3-8, each-O-(CHRd) z-is a group-OCH (CH3) CH2- and x is 1-3. In this group the polyether chain of -OCH (CH3) CH2- groups (a polyoxypropylene chain) forms the hydrophobic moiety of the surfactant which is linked via one or more oxyethylene units to the amino function. In preferred surfactants of this group, x is 1, and Rf, Rg and Rh are independently Cl 4 alkyl. These surfactants are a subclass of the polyoxypropylene quaternary ammonium salts disclosed in U. S. Patent No. 5,652,197. In a suitable example, y is 7, x is 1, Rf, R'and R are each methyl, ZP-is a chloride anion and p is 1.

(E) Surfactants of formula (IV) where Re is a Cg- ; 8 aliphatic, saturated or unsaturated, linear or branched hydrocarbyl chain, x is 0, y is 0 and Rs, Rt and Ru are independently- (CH2CH2-O) ; H groups where each k is an average number such that k is 1 to about 50. This type includes surfactants known in the art or referred to herein as polyoxyethylene N-alkyl alkylenediamines. An illustrative example is polyoxyethylene N-oleyl-1,3-diaminopropane having 3 oxyethylene units per molecule, as disclosed in French Patent Application No. 2 648 316.

In surfactants of formula (II), ZP-can be any suitable anion but preferably is chloride, bromide, iodide, sulfate, ethosulfate, phosphate, acetate, propionate, succinate, lactate, citrate or tartrate, or, as indicated above, an anion of the anionic exogenous chemical substance, for example glyphosate.

Presently the most preferred amine surfactants for use in compositions of the invention are polyoxyethylene (2-20) Cl2 alkylamines and alkylammonium chlorides.

Amounts and ratios of alkvlether and amine surfactants The present invention is based in part on the unexpected discovery that biological effectiveness of an anionic exogenous chemical substance such as glyphosate herbicide in the presence of a mixture of alkylether and amine surfactants as defined herein can be significantly greater than that obtained in the presence of either the alkylether or the amine surfactant alone, at the same total surfactant concentration. The ratio of the alkylether surfactant component to the amine surfactant component producing this surprisingly synergistic interaction is not narrowly critical between about 1: 10 and about 10: 1 by weight. However, it will generally be found suitable to provide a ratio of about 1: 5 to about 5: 1, for example about 1: 3 to about 3: 1. An

optimum ratio for particular surfactants, with a particular anionic exogenous chemical substance under particular conditions, can readily be determined by one of skill in the art by routine experimentation.

The present invention provides the greatest benefit where the amount of surfactant relative to the amount of anionic exogenous chemical substance applied is fairly low, specifically no higher than about 0.5 parts by weight of surfactant per part by weight of the anionic exogenous chemical substance, expressed as acid equivalent. At higher surfactant levels, typically either one of the surfactant components alone imparts a high degree of biological effectiveness and no great further advantage accrues from use of the mixture. At a surfactant rate lower than about 0.05 part by weight per part by weight of the anionic exogenous chemical substance, the synergistic interaction can be evident but the total amount of surfactant is normally insufficient to provide a useful magnitude of enhancement in biological effectiveness.

For example, where the anionic exogenous chemical substance is glyphosate, the greatest benefit of the invention is typically realized at a weight ratio of surfactant to glyphosate a. e. of about 0.1: 1 to about 0.4: 1.

In a plant treatment composition useful in the method of the invention, the concentration of alkylether and amine surfactants together is preferably not greater than about 7.5 g/l, although higher concentrations can be used if desired. More preferably, the concentration of alkylether and amine surfactants together is about 0.5 to about 5 g/l, for example about 1 to about 3 g/l.

In a liquid concentrate composition useful in practicing the invention, the concentration of alkylether and amine surfactants together is typically about 25 to about 250 g/I, more typically about 50 to about 150 g/l. In a solid concentrate composition useful in practicing the invention, the concentration of alkylether and amine surfactants together is typically about 3% to about 30% by weight, more typically about 6% to about 18% by weight.

Other ingredients Compositions useful in the method of the invention can contain agriculturally acceptable materials other than an anionic exogenous chemical substance or salt thereof, an alkylether surfactant and an amine surfactant.

For example, more than one anionic exogenous chemical substance can be included. For example, a glyphosate composition of the invention can optionally contain, in addition to glyphosate, any other anionic herbicide selected from those hereinbefore listed.

In a particular embodiment of the invention, glyphosate is present in a plant treatment composition together with a second anionic herbicide which is normally antagonistic to the biological effectiveness of glyphosate. The second anionic herbicide is typically one that produces visible symptoms of phytotoxicity in a treated plant within about 4 days after application to foliage. In preferred compositions useful in this embodiment, the second anionic herbicide is glufosinate in the form of a salt or salts thereof. Glufosinate typically elicits symptoms of phytotoxicity in a treated plant more rapidly than glyphosate and often antagonizes the longer-term herbicidal effectiveness of glyphosate when the two herbicides are co-applied in a formulation or tank-mixture of prior art. Surprisingly and by contrast, such antagonism has generally been found to be substantially reduced when glyphosate and glufosinate are accompanied by an alkylether and an amine surfactant in accordance with the present invention.

It is contemplated that reduction in antagonism is a feature of compositions containing any two anionic exogenous chemical substances, one of which normally antagonizes the biological effectiveness of the other.

Illustratively in a composition useful in the method of the invention containing glyphosate and a second anionic herbicide, the a. e. weight ratio of the second anionic herbicidal substance, e. g., glufosinate, to glyphosate can be about 1: 1 to about 1: 30, preferably about 1: 2 to about 1: 20.

A herbicidal composition useful in the method of the invention can optionally contain, in addition to an anionic herbicidal compound such as glyphosate or a salt thereof, a herbicidal compound that is other than anionic, such as for example an ester derivative of an anionic herbicide, or a herbicide selected from acetochlor, aclonifen, alachlor, ametryn, amidosulfuron, anilofos, atrazine, azafenidin, azimsulfuron, benfluralin, benfuresate, bensulfuron-methyl, bensulide, benzofenap, bifenox, bromobutide, bromofenoxim, butachlor, butamifos, butralin, butroxydim, butylate, cafenstrole, carbetamide, carfentrazone-ethyl, chlomethoxyfen, chlorbromuron, chloridazon, chlorimuron-ethyl, chlorotoluron, chlornitrofen, chlorotoluron, chlorpropham, chlorsulfuron, chlorthal-dimethyl, chlorthiamid, cinmethylin, cinosulfuron, clethodim, clodinafop-propargyl, clomazone, clomeprop, cloransulam-methyl, cyanazine, cycloate, cyclosulfamuron, cycloxydim, cyhalofop-butyl, daimuron, desmedipham, desmetryn, dichlobenil, diclofop-methyl, diflufenican, dimefuron, dimepiperate, dimethachlor, dimethametryn, dimethenamid, dinitramine, dinoterb, diphenamid, dithiopyr, diuron, EPTC,

esprocarb, ethalfluralin, ethametsulfuron-methyl, ethofumesate, ethoxysulfuron, etobenzanid, fenoxaprop-ethyl, fenuron, flamprop-methyl, flazasulfuron, fluazifop-butyl, fluchloralin, flumetsulam, flumiclorac-pentyl, flumioxazin, fluometuron, fluorochloridone, fluoroglycofen- ethyl, flupoxam, flurenol, fluridone, fluroxypyr-1-methylheptyl, flurtamone, fluthiacet-methyl, fomesafen, halosulfuron, haloxyfop-methyl, hexazinone, imazosulfuron, indanofan, isoproturon, isouron, isoxaben, isoxaflutole, isoxapyrifop, lactofen, lenacil, linuron, mefenacet, metamitron, metazachlor, methabenzthiazuron, methyldymron, metobenzuron, metobromuron, metolachlor, metosulam, metoxuron, metribuzin, metsulfuron, molinate, monolinuron, naproanilide, napropamide, naptalam, neburon, nicosulfuron, norflurazon, orbencarb, oryzalin, oxadiargyl, oxadiazon, oxasulfuron, oxyfluorfen, pebulate, pendimethalin, pentanochlor, pentoxazone, phenmedipham, piperophos, pretilachlor, primisulfuron, prodiamine, prometon, prometryn, propachlor, propanil, propaquizafop, propazine, propham, propisochlor, propyzamide, prosulfocarb, prosulfuron, pyraflufen-ethyl, pyrazolynate, pyrazosulfuron-ethyl, pyrazoxyfen, pyributicarb, pyridate, pyriminobac-methyl, quinclorac, quinmerac, quizalofop-ethyl, rimsulfuron, sethoxydim, siduron, simazine, simetryn, sulcotrione, sulfentrazone, sulfometuron, sulfosulfuron, tebutam, tebuthiuron, terbacil, terbumeton, terbuthylazine, terbutryn, thenylchlor, thiazopyr, thifensulfuron, thiobencarb, tiocarbazil, tralkoxydim, triallate, triasulfuron, tribenuron, trietazine, trifluralin, triflusulfuron and vernolate.

In a particular embodiment of the invention, glyphosate is present in a plant treatment composition together with a second herbicide that is other than anionic and is normally antagonistic to the biological effectiveness of glyphosate. Typically the second herbicide is one that produces visible symptoms of phytotoxicity in a treated plant within about 4 days after application to foliage. In preferred compositions useful in this embodiment, the second herbicide is selected from carfentrazone-ethyl, flumiclorac-pentyl, flumioxazin, fluoroglycofen-ethyl, fomesafen, lactofen and oxyfluorfen. Surprisingly, the antagonism of glyphosate herbicidal effectiveness often seen in prior art compositions or tank-mixtures with these herbicides has generally been found to be substantially reduced when glyphosate and the second herbicide, for example oxyfluorfen, are accompanied by an alkylether and an amine surfactant in accordance with the present invention.

Illustratively in a composition of the invention containing glyphosate and a second herbicide that is other than anionic, the weight ratio of the second herbicide, e. g., carfentrazone-

ethyl, to glyphosate a. e. can be about 1: 1 to about 1: 250. Where the second herbicide, e. g., oxyfluorfen, is required in higher dosage amounts to produce visible symptoms of phytotoxicity, the weight ratio of the second herbicide to glyphosate a. e. is preferably about 1: 1 to about 1: 30, more preferably about 1: 2 to about 1: 20.

Exogenous chemical substances useful in compositions of the invention can be selected from those listed in standard reference works such as The Pesticide Manual, 11th Edition, British Crop Protection Council (1997), and Farm Chemicals Handbook'97, Meister Publishing Company (1997).

Various agriculturally acceptable adjuvants or excipient substances can also be included, whether or not their purpose is to contribute directly to the biological effectiveness of an exogenous chemical substance in a treated plant. For example, where the exogenous chemical substance is a herbicide, liquid nitrogen fertilizer or ammonium sulfate can be included in the composition. Ammonium sulfate and certain other inorganic ammonium salts are known to enhance herbicidal effectiveness of glyphosate and other herbicides on certain plant species.

Other optional components of compositions useful in the method of the invention include agents to modify color, odor, viscosity, gelling properties, freezing point, stability or texture.

One or more surfactant (s), other those of the classes specifically described above, can also be included in a contemplated composition. A wide range of surfactants is available to the formulator of exogenous chemical substances and can be selected readily from standard works such as McCutcheon's Emulsifiers and Detergents, 1998 Edition, MC Publishing Company, or Handbook of Industrial Surfactants, 2nd Edition, Gower (1997).

There is no restriction on the type or chemical class of such additional surfactant that can be used. Nonionic, anionic, cationic and amphoteric types, or combinations of more than one of these types, are all useful in particular situations. For example, soybean lecithin, which contains amphoteric acyl phosphatidylcholine surfactants, has been found useful in stabilizing certain liquid concentrate compositions as more particularly described below.

Another class of excipient material that can be useful in compositions useful in the method of the present invention is an oil, such as a triglyceride ester of fatty acids of animal, vegetable or synthetic origin, a paraffin, a polysiloxane, or a fatty acid or an ester or amide thereof. Natural triglyceride oils can be fractionated or not. Fractionation permits elimination of certain fatty acid chain lengths so as to modify melting point.

In a particular embodiment of the invention, one or more oil (s) are included, each having a chemical structure corresponding to formula (V): wherein R4 is a hydrocarbyl group having about 5 to about 21 carbon atoms, R5 is a hydrocarbyl group having 1 to about 14 carbon atoms, the total number of carbon atoms in R4 and R5 is about 11 to about 27, and Q is O or NH. R4 and R are preferably linear hydrocarbyl chains. R4 preferably has about 11 to about 21 carbon atoms and is preferably derived from a natural saturated or unsaturated fatty acid. R5 is preferably an alkyl group with 1 to about 6 carbon atoms. Especially preferred oils of formula (V) are therefore Cri-6 alkylesters or C 1-6 alkylamides of fatty acids.

In certain preferred embodiments, an oil is included that is a Cl 4 alkylester of a C 12-18 fatty acid, more preferably a Cl 4 alkylester of a Cl218 saturated fatty acid. Examples include methyl oleate, ethyl oleate, isopropyl myristate, isopropyl palmitate and butyl stearate. Butyl stearate is especially preferred.

When present, one or more oil (s) of formula (V) are preferably included in a ratio of total weight of such oil (s) to weight of the anionic exogenous chemical substance, expressed as acid equivalent, of about 1: 100 to about 1: 1, though greater or lesser amounts can be found useful in particular situations.

Suitable concentrations of an oil of formula (V), if present, are about 0.001 % to about 0.1% by weight in a plant treatment composition, and about 0.05% to about 5% by weight in a liquid concentrate composition. Higher or lower concentrations can be useful in particular situations.

Oil (s), if present, can be emulsified in a composition by means of the alkylether and/or amine surfactants. If desired, additional surfactant (s) can be included as emulsifier (s) for such oil (s).

Glycols form another class of excipient that can optionally be present in compositions useful in the method of the invention. For example, diethylene glycol and/or propylene glycol can be present as antifreezes, pour point depressants or in some other role. Polyglycols, such as polyethylene glycol having a molecular weight in the range from about 200 to about 800, especially about 400 to about 600, can also be useful for similar functions and/or as aids to inhibit gelling of the amine surfactant.

In preparing highly concentrated aqueous compositions useful in practicing the invention, certain coupling agents may be found beneficial in enhancing stability. These include low molecular weight alcohols such as ethanol, isopropanol and butanol, also dimethyl sulfoxide (DMSO), urea and tetrabutylammonium hydroxide.

Yet another class of excipient that can optionally be present is a solid microparticulate or nanoparticulate substance such as silica, which can serve as a stabilizer and/or thickener in liquid concentrate compositions.

Concentrate compositions Although a plant treatment composition as described above can be prepared on-site as a dilute aqueous solution or dispersion immediately before application to foliage of a plant, a preferred embodiment of the invention involves dissolution, dispersion or dilution of a storage- stable concentrate composition. When dissolved or dispersed in, or diluted with, a suitable amount of water, such a concentrate composition forms a plant treatment composition useful in the method of the invention as described above. Thus the ratios of ingredients other than water defined for a plant treatment composition herein apply equally to a concentrate composition.

Typically in preparing a plant treatment composition, one part by weight of a concentrate composition is added to about 9 to about 99 parts by weight of water; however greater or lesser amounts of water can be useful in particular situations.

Concentrate compositions useful in practicing the invention can be solid or liquid.

Formulation types known in the art to be generally suitable for foliar-applied anionic exogenous chemical substances are useful for the present invention. These include, without restriction, concentrated aqueous solutions and dispersions, emulsions (including oil-in-water, water-in-oil and water-in-oil-in-water types), microemulsions, suspension concentrates, emulsifiable concentrates, suspoemulsions, wettable powders, water-soluble powders and granules, water- dispersible powders and granules, etc.

A solid concentrate composition useful in practicing the invention, such as a water- soluble or water-dispersible granule formulation, contains in total at least about 10% by weight and up to about 90% by weight of an anionic exogenous chemical substance expressed as acid equivalent. Preferably the content of exogenous chemical substance in a solid concentrate composition is about 25% to about 75%, more preferably about 50% to about 75%, a. e. by weight. Solid compositions are sometimes referred to as"dry"formulations; this should not be

taken to imply that such compositions are entirely free of water or other liquid, merely that they feel dry to the touch.

In accordance with one embodiment of the invention, a solid concentrate composition consists essentially of an anionic exogenous chemical substance or salt thereof, an alkylether surfactant and an amine surfactant as herein defined. In accordance with another embodiment of the invention, a solid concentrate composition comprises these same ingredients together with other excipients. In accordance with a particular embodiment of the invention, a solid concentrate composition comprises an anionic exogenous chemical substance or salt thereof, an alkylether surfactant and an amine surfactant as herein defined, and ammonium sulfate.

Preferred solid concentrate compositions are water-soluble or water-dispersible granules.

A liquid concentrate composition useful in practicing the invention, such as an aqueous solution or dispersion, contains in total at least about 10% by weight and up to about 50% or more by weight of an anionic exogenous chemical substance expressed as acid equivalent.

Preferably the content of anionic exogenous chemical substance in a liquid concentrate composition is about 15% to about 45%, more preferably about 20% to about 40%, a. e. by weight. Weight/volume concentrations will vary depending on specific gravity of the liquid composition; however, typically the anionic exogenous chemical substance is present at about 180 to about 540 g a. e./l, more typically about 240 to about 480 g a. e./l.

Preferred liquid concentrate compositions have a continuous aqueous phase wherein is dissolved the anionic exogenous chemical substance in the form of a water-soluble salt thereof, forming an aqueous solution. The alkylether surfactant typically does not dissolve readily in this aqueous solution and instead forms a dispersed phase. The amine surfactant can be associated with the dispersed phase or distributed in the aqueous phase (for example as micelles) or both.

The alkylether surfactant dispersion in such preferred compositions is stabilized by an emulsifying system, in which the amine surfactant can play a role. In one embodiment, the emulsifying system comprises acyl phosphatidylcholine, for example in the form of soybean lecithin, and an oil of formula (V) above, such as butyl stearate. Optionally a coupling agent such as a low molecular weight alcohol, DMSO, urea or tetrabutylammonium hydroxide can be included to enhance stability. This can be found especially beneficial when it is desired to increase the concentration of the anionic exogenous chemical substance to a high level, for example, in the case of glyphosate, above about 24% a. e. by weight.

Illustratively, a concentrate composition useful in this embodiment of the invention, wherein the anionic exogenous chemical substance is glyphosate in the form of its isopropylammonium (IPA) salt, the alkylether surfactant is ceteareth-27 (e. g., Plurafac A-38 of BASF) and the amine surfactant is polyoxyethylene (15) tallowamine (e. g., Ethomeen T/25 of Akzo), has the following ingredients (all percentages are by weight): glyphosate salt 24-48% (18-36% a. e.) ceteareth-27 2-10% polyoxyethylene (15) tallowamine 2-10% soybean lecithin 1-10% butyl stearate 0.5-10% coupling agent 0-5% water to 100% In liquid concentrate compositions useful in this embodiment of the invention, a fraction of the anionic exogenous chemical substance, for example glyphosate, is typically strongly associated with, or entrapped by, the dispersed phase. When the composition is diluted with water for foliar application, the fraction associated with or entrapped by the dispersed phase greatly declines; however, upon evaporation of water from a spray deposit on a leaf surface it is believed that association with or entrapment by supramolecular surfactant structures occurs again.

The association of glyphosate with supramolecular surfactant structures in liquid concentrate compositions of this embodiment is believed to be correlated with physical stability of the composition. Less physically stable compositions, for example those in which phase separation occurs within 24 hours when stored without agitation at 20-25°C, and in particular those with a relatively high weight ratio of amine surfactant to lecithin, typically show a lesser degree of glyphosate association with or entrapment by supramolecular surfactant structures.

Without being bound by theory, it is theorized that the strong association between glyphosate and supramolecular surfactant structures in compositions of this embodiment of the invention plays a role in enhancing glyphosate uptake across leaf cuticles. The association or entrapment is readily detectable by NMR spectroscopy techniques. One such technique involves the following illustrative procedure.

A sample of the liquid concentrate composition, conveniently about 200 to about 500 1, is placed in an NMR tube. A diffusion probe is used having a gradient coil capable of generating a linear field gradient of about 250 gauss/cm across the sample in response to a current pulse of 20 amp. Proton NMR spectra are recorded as a function of increasing field gradient. Data are collected using bipolar pulses and LEDS pulse sequence to measure diffusion by the pulse field gradient method of Wu et al., Journal of Magnetic Resonance, A115,260-264,1995.

The glyphosate resonance measured in the present procedure is that associated with the methylene group adjacent to the phosphonate moiety of the glyphosate molecule, which is well known in the art. Integrated intensity (amplitude) of the glyphosate resonance is measured in each spectrum and the natural logarithm of such amplitude is plotted against the square of the field gradient. Data falling on a straight line in such a plot are indicative of simple diffusion in a one-component system. Data falling on a curve that can be resolved into two straight-line components, as in Figure 1, are indicative of a two-component system having two pools of glyphosate that diffuse at different speeds. The faster-diffusing glyphosate represents the"free" pool, i. e., glyphosate present in the aqueous medium, and the slower-diffusing glyphosate represents the"entrapped"pool, i. e., glyphosate strongly associated with or entrapped by supramolecular aggregates formed by surfactants. The relative size of the two pools can be estimated by extrapolating the linear fit of each straight-line component to zero field gradient (the y-axis in the graph of Figure 1). The amount of glyphosate in a pool is proportional to the antilogarithm of the corresponding y-intercept value.

Certain aqueous concentrate compositions useful in practicing the invention can be described as stable dispersions. By"stable"in this context it is meant that no phase separation occurs during storage of a composition without agitation at 20-25°C for 24 hours. The more desirable aqueous concentrate compositions are dispersions in which no phase separation occurs during storage without agitation at constant or varying temperatures from about 10°C to about 40°C for 48 hours, even more desirably from about 0°C to about 50°C for 7 days, and most desirably about-10°C to about 60°C for 30 days. Stability at elevated temperatures for short time periods provides a good indication of long-term stability under normal storage conditions; it is contemplated that certain concentrate compositions useful in practicing the invention will be stable for periods of 1 year or more under normal storage conditions.

Process for making a liquid concentrate composition Liquid concentrate compositions can be prepared by mixing the ingredients together in a suitable vessel. The degree of agitation required depends on the precise ingredients as will be understood by those of skill in the art; it will likewise be recognized that certain ingredients require special treatment.

An aqueous concentrate composition containing lecithin and butyl stearate, as described above, can be prepared in the following illustrative way.

Lecithin is added to water in a suitable vessel and is fan-mixed, for example with a Variac mixer, set at 30% of maximum voltage, for about 10 minutes. This results in the lecithin becoming hydrated. It is preferred to hydrate lecithin at an elevated temperature, for example around 50°C. To the hydrated lecithin in water are then added, in any order, the anionic exogenous chemical substance in the form of a water-soluble salt thereof, the alkylether surfactant, the amine surfactant and the butyl stearate. The vessel containing the resulting mixture is first agitated moderately, e. g., by hand shaking in the case of a small-scale preparation, and is then subjected to more vigorous mixing until homogeneous. This can be accomplished, for example, by fan-mixing with a Variac mixer, set at 30% of maximum voltage, for about 10 minutes. Alternatively, it can be accomplished by mixing with a Turrax mixer at 20,000 rpm for about 8 minutes. Optionally, the composition can then be microfluidized, for example using a Model M-11 OF microfluidizer of Microfluidics International Corp., for 5 cycles at 15, 000 psi (69 MPa).

Liquid concentrate compositions useful in practicing the invention are not limited to those prepared by the above procedure or a variant thereof. Other processes suitable for making a liquid concentrate composition are described in the Examples or can be developed by one of skill in the art by routine experimentation.

Process for making a solid concentrate composition A process for preparing a solid concentrate composition useful in practicing the invention comprises a first step of mixing an anionic exogenous chemical substance, or a salt thereof, or a mixture of such anionic exogenous chemical substance and salt thereof, in solid particulate form with an alkylether surfactant and an amine surfactant, and optionally with other desired ingredients, together with sufficient water to form a wet mix of consistency suitable for further

process steps as described immediately below. The alkylether surfactant can be used in powder form or it can be melted prior to addition to the wet mix.

Such a process further comprises a second step of granulating the wet mix to form moist coherent granules, and a third step of drying the granules. Any granulating method known in the art to be suitable for the preparation of water-soluble or water-dispersible granules of an exogenous chemical substance can be used; preferred methods are pan granulation and extrusion granulation. The extrusion process described in United Kingdom Patent Application No.

1 433 882 is one illustrative process that can be useful in preparing granular compositions useful in practicing the present invention. Any drying method known in the art to be suitable for the preparation of water-soluble or water-dispersible granules of an exogenous chemical substance can be used; a preferred method is fluid-bed drying.

Solid concentrate compositions useful in practicing the invention are not limited to those prepared by the above procedure or a variant thereof. Other processes suitable for making a solid concentrate composition can be developed by one of skill in the art by routine experimentation.

Application of a plant treatment composition to foliage Exogenous chemical substances are applied to plants at a rate sufficient to give the desired effect. These application rates are usually expressed as amount of exogenous chemical substance per unit area treated, e. g. grams per hectare (g/ha). What constitutes a"desired effect" varies according to the standards and practice of those who investigate, develop, market and use a specific class of exogenous chemical substances. For example, in the case of a herbicide, the amount applied per unit area to give, consistently and reliably, at least 85% control of a plant species as measured by growth reduction or mortality is often used to define a commercially effective rate.

Herbicidal effectiveness is one of the biological effects that can be enhanced through this invention."Herbicidal effectiveness,"as used herein, refers to any observable measure of control of plant growth, which can include one or more of the actions of (1) killing, (2) inhibiting growth, reproduction or proliferation, and (3) removing, destroying, or otherwise diminishing the occurrence and activity of plants.

The selection of application rates that are biologically effective for a specific anionic exogenous chemical substance is within the skill of the ordinary agricultural scientist. Those of

skill in the art will likewise recognize that individual plant conditions, weather and growing conditions, as well as the specific anionic exogenous chemical substance and composition thereof selected, will influence the degree of biological effectiveness achieved in practicing this invention. Useful application rates for anionic exogenous chemical substances employed can depend upon all of the above conditions. With respect to the use of the method of this invention for glyphosate herbicide, much information is known about appropriate application rates. Over two decades of glyphosate use and published studies relating to such use have provided abundant information from which a weed control practitioner can select glyphosate application rates that are herbicidally effective on particular species at particular growth stages in particular environmental conditions.

Herbicidal compositions of glyphosate or derivatives thereof are used to control a very wide variety of plants worldwide. Glyphosate compositions in accordance with the invention can be applied to a plant in a herbicidally effective amount, and can effectively control one or more plant species of one or more of the following genera without restriction: Abutilon, Amaranthus, Artemisia, Asclepias, Avena, Axonopus, Borreria, Brachiaria, Brassica, Bromus, Chenopodium, Cirsium, Commelina, Convolvulus, Cynodon, Cyperus, Digitaria, Echinochloa, Eleusine, Elymus, Equisetum, Erodium, Helianthus, Imperata, Ipomoea, Kochia, Lolium, Malva, Oryza, Ottochloa, Panicum, Paspalum, Phalaris, Phragmites, Polygonum, Portulaca, Pteridium, Pueraria, Rubus, Salsola, Setaria, Sida, Sinapis, Sorghum, Triticum, Typha, Ulex, Xanthium and Zea.

Particularly important annual broadleaf species for which glyphosate compositions are used are exemplified without limitation by the following: velvetleaf (Abutilon theophrasti), pigweed (Amaranthus spp.), buttonweed (Borreria spp.), oilseed rape, canola, indian mustard, etc. (Brassica spp.), commelina (Commelina spp.), filaree (Erodium spp.), sunflower (Helianthus spp.), morningglory (Ipomoea spp.), kochia (Kochia scoparia), mallow (Malva spp.), wild buckwheat, smartweed, etc. (Polygonum spp.), purslane (Portulaca spp.), russian thistle (Salsola spp.), sida (Sida spp.), wild mustard (Sinapis arvensis) and cocklebur (Xanthium spp.) Particularly important annual narrowleaf species for which glyphosate compositions are used are exemplified without limitation by the following: wild oat (Avena fatua), carpetgrass (Axonopus spp.), downy brome (Bromus tectorum), crabgrass (Digitaria spp.), barnyardgrass (Echinochloa crus-galli), goosegrass (Eleusine indica), annual ryegrass (Lolium multiflorum),

rice (Oryza sativa), ottochloa (Ottochloa nodosa), bahiagrass (Paspalum notatum), canarygrass (Phalaris spp.), foxtail (Setaria spp.), wheat (Triticum aestivum) and corn (Zea mays).

Particularly important perennial broadleaf species for which glyphosate compositions are used are exemplified without limitation by the following: mugwort (Artemisia spp.), milkweed (Asclepias spp.), canada thistle (Cirsium arvense), field bindweed (Convolvulus arvensis) and kudzu (Pueraria spp.).

Particularly important perennial narrowleaf species for which glyphosate compositions are used are exemplified without limitation by the following: brachiaria (Brachiaria spp.), bermudagrass (Cynodon dactylon), yellow nutsedge (Cyperus esculentus), purple nutsedge (C. rotundus), quackgrass (Elynzus repens), lalang (Imperata cylindrica), perennial ryegrass (Lolium prenne), guineagrass (Panicum maximum), dallisgrass (Paspalum dilatatum), reed (Phragmites spp.), johnsongrass (Sorghum halepense) and cattail (Typha spp.).

Other particularly important perennial species for which glyphosate compositions are used are exemplified without limitation by the following: horsetail (Equisetum spp.), bracken (Pteridium aquilinum), blackberry (Rubus spp.) and gorse (Ulex europaeus).

Thus, the method of the invention where glyphosate is the anionic exogenous chemical substance can be useful on any of the above species. In a particular embodiment, a plant treatment composition as provided herein comprising glyphosate in the form of one or more salt (s) thereof is applied to foliage of crop plants genetically transformed to tolerate glyphosate, and simultaneously to foliage of weeds or undesired plants growing in close proximity to such crop plants. This process results in control of the weeds or undesired plants while leaving the crop plants substantially unharmed. Crop plants genetically transformed to tolerate glyphosate include those whose seeds are sold by Monsanto or under license from Monsanto bearing the Roundup ReadyX trademark. These include varieties of cotton, soybean, canola and corn.

Application of plant treatment compositions to foliage of plants is preferably accomplished by spraying, using any conventional means for spraying liquids, such as spray nozzles or spinning-disk atomizers. The method of the present invention can be used in precision farming techniques, in which apparatus is employed to vary the amount of exogenous chemical substance applied to different parts of a field, depending on variables such as the particular plant species present, plant growth stage, soil moisture status, etc. In one embodiment

of such techniques, a global positioning system operated with the spraying apparatus can be used to control application of the composition in desired amounts to different parts of a field.

A plant treatment composition is preferably dilute enough to be readily sprayed using standard agricultural spray equipment. Suitable application rates for the present invention vary depending upon a number of factors, including the type and concentration of active ingredient and the plant species involved. Useful rates for applying an aqueous composition to a field of foliage can range from about 25 to about 1,000 liters per hectare (1/ha), preferably about 50 to about 300 1/ha, by spray application.

EXAMPLES The following Examples are provided for illustrative purposes only and are not intended to limit the scope of the present invention. The Examples will permit better understanding of the invention and perception of its advantages and certain variations of execution.

The following procedure was used for testing compositions of the Examples to determine herbicidal effectiveness, except where otherwise indicated.

Seeds of the plant species indicated were planted in 85 mm square pots in a soil mix which was previously steam sterilized and prefertilized with a 14-14-14 NPK slow release fertilizer at a rate of 3.6 kg/m3. The pots were placed in a greenhouse with sub-irrigation. About one week after emergence, seedlings were thinned as needed, including removal of any unhealthy or abnormal plants, to create a uniform series of test pots.

The plants were maintained for the duration of the test in the greenhouse where they received a minimum of 14 hours of light per day. If natural light was insufficient to achieve the daily requirement, artificial light with an intensity of approximately 475 microeinsteins was used to make up the difference. Exposure temperatures were not precisely controlled but averaged about 27°C during the day and about 18°C during the night. Plants were sub-irrigated throughout the test to ensure adequate soil moisture levels.

Pots were assigned to different treatments in a fully randomized experimental design with 3 replications. A set of pots was left untreated as a reference against which effects of the treatments could later be evaluated.

Application of glyphosate compositions was made by spraying with a track sprayer fitted with a 9501E nozzle calibrated to deliver a spray volume of 93 1/ha at a pressure of 166 kPa.

After treatment, pots were returned to the greenhouse until ready for evaluation.

Treatments were made using dilute aqueous compositions. These could be prepared as spray compositions directly from their ingredients, or by dilution with water of preformulated concentrate compositions. All comparisons were made at equal glyphosate acid equivalent rates.

The required degree of dilution for a glyphosate concentrate composition to make a plant treatment composition is calculated from the equation A = RS/VC where A is the volume in milliliters (ml) of the glyphosate composition to be added to the plant treatment composition being prepared, R is the desired glyphosate rate in grams of acid equivalent per hectare (g a. e./ha), S is the total volume in milliliters (ml) of plant treatment composition being prepared, V is the application rate in liters per hectare (1/ha) of plant treatment composition, conventionally referred to as"spray volume", and C is the concentration of glyphosate in grams of acid equivalent per liter (g a. e./l) in the glyphosate composition.

For evaluation of herbicidal effectiveness, all plants in the test were examined by a single practiced technician, who recorded percent inhibition, a visual measurement of the effectiveness of each treatment by comparison with untreated plants. Inhibition of 0% indicates no effect, and inhibition of 100% indicates that all of the plants are completely killed. Inhibition of 85% or more is in most cases considered acceptable for normal herbicidal use; however in greenhouse tests such as those of the Examples it is normal to include rates which give less than 85% inhibition, as this makes it easier to discriminate among compositions having different levels of effectiveness.

Example 1 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table la. CS-20 of Heterene is ceteareth-20 (polyoxyethylene C16-18 alkylether having an average of 20 oxyethylene units per molecule). Plurafac A-38 of BASF is ceteareth-27 (polyoxyethylene C16-18 alkylether having an average of 27 oxyethylene units per molecule). Ethomeen T/25 of Akzo is polyoxyethylene tallowamine having an average of 15 oxyethylene units per molecule. The lecithin used was a soybean lecithin product of Avanti containing 45% phospholipid. All compositions of this Example are oil-in-water emulsions and were prepared by the following process.

Lecithin was first hydrated and dispersed in water as a 15% by weight stock by sonication using a Fisher Sonic Dismembrator, Model 550, fitted with a 2.4 cm probe tip with

the pulse period set at 15 seconds with 1 minute intervals between pulses to allow cooling.

Power output was set at level 8. Sonication was continued for 3 minutes (12 pulse periods).

Next, the required amounts of butyl stearate, lecithin and alkylether and/or amine surfactants were mixed thoroughly together, along with additional water if necessary. The alkylether surfactant was heated to bring it into a flowable condition before mixing. Then the required amount of glyphosate IPA salt (in the form of MON 0139, a 62% by weight aqueous solution of glyphosate IPA salt) was added to the resulting mixture with further agitation. The required amount of water was added to bring the concentration of glyphosate and other ingredients to the desired level. The composition was finally subjected to high-shear mixing using a Silverson L4RT-A mixer fitted with a medium emulsor screen, operated for 3 minutes at 7,000 rpm.

Table la Concentrate Glyphosate Weight % composition g a. e./l Lecithin Butyl Ethomeen CS-20 Plurafac stearateT/25 A-38 0.750.751.51-01220 1-02 220 0.75 0.75 1.5 1-03 220 0. 75 0. 75 3. 0 1-04 220 0. 75 7. 50 1. 5 1-05 220 0. 75 7. 50 3. 0 1-06 220 3. 75 3. 75 3. 0 1-07 220 1. 50 1. 50 3. 0 1-08 220 1. 50 1. 50 1. 5 1-09 220 3. 75 3. 75 1. 5 1. 5 1-10 220 1. 50 1. 50 1. 5 1. 5 3.757.501.51.51-11220 1-12 220 3. 75 1. 50 1. 5 1. 5 1-13 220 0. 75 3. 75 1. 5 1. 5 1-14 220 0. 75 7. 50 1. 5 1. 5 1-15 3.753.03.00.75 Concentrate Glyphosate Weight % composition g a. e./l Lecithin Butyl Ethomeen CS-20 Plurafac stearate T/25 A-38 1-16 220 0.75 7.50 3.0 3.0 1-17 220 7. 50 3. 0 1-18 220 0. 75 7. 50 3. 0

Glyphosate concentration in each of the above compositions was approximately 20% a. e. by weight. Thus the weight ratio of alkylether + amine surfactant to glyphosate a. e. was approximately 0.3: 1 where total alkylether + amine surfactant concentration was 6% by weight, and approximately 0.15: 1 where total alkylether + amine surfactant concentration was 3% by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet, a form of barnyardgrass (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 23 days after planting ABUTH and ECHCF, and evaluation of herbicidal effectiveness was done 18 days after application.

Two commercial concentrate formulations of glyphosate were diluted and applied as standard comparative treatments. These were Accord) herbicide of Monsanto, which consists essentially of 480 g/1 glyphosate IPA salt (approximately 360 g a. e./l) in aqueous solution, and Roundup (E) Ultra herbicide of Monsanto, which contains 480 g/1 glyphosate IPA salt (approximately 360 g a. e./1) in aqueous solution together with surfactant.

Results, averaged for all replicates of each treatment, are shown in Table Ib.

Table lb Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF Accord (t 100 12 62 200 5 55 300 23 63 400 43 78 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 1002782Roundup#Ultra 200 62 98 300 88 95 400 96 99 1-01 100 13 79 200 68 95 300 82 99 400 95 91 1-02 100 27 82 200 60 97 300 81 95 400 87 99 1-03 100 37 77 200 62 96 300 78 98 400 89 90 1-04 100 37 84 200 57 95 300 84 99 400 89 100 33771-05100 200 65 100 300 78 97 400 88 97 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 1-06 100 43 78 200 62 95 300 87 97 400 95 96 1-07 100 48 78 200 80 91 300 90 99 400 76 93 1-08 100 48 83 200 67 89 300 86 96 400 93 97 1-09 100 62 84 200 82 98 300 85 99 400 91 97 1-10 100 63 80 200 75 96 300 85 99 400 99 99 1-11 100 42 75 200 78 98 300 92 99 400 93 100 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 1-12 100 52 80 200 73 93 300 86 99 400 97 97 1-13 100 55 83 200 75 97 300 97 99 400 92 99 1-14 100 52 87 200 73 95 300 91 97 400 87 98 1-15 100 57 83 200 92 96 300 98 100 400 100 98 1-16 100 79 88 200 87 97 300 99 99 400 97 94 1-17 100 58 83 200 47 94 300 88 98 400 91 93 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 1-18 100 58 87 200 75 91 300 83 99 400 91 98

Outstanding herbicidal effectiveness was provided by composition 1-18, containing 3% of the alkylether surfactant Plurafac A-38. Addition of 3% of the amine surfactant Ethomeen T/25 (composition 1-16) further enhanced effectiveness. Composition 1-14, wherein the content of alkylether and amine surfactants were reduced to 1.5% each, surprisingly performed substantially equally to composition 1-18 (3% alkylether, no amine) and better than composition 1-05 (no alkylether, 3% amine). In all these compositions, lecithin content was 0.75% and butyl stearate content was 7.5%.

Perhaps because of the relatively high content of butyl stearate in these compositions, herbicidal effectiveness elicited by the alkylether surfactant was so high that a truly synergistic interaction between the alkylether and the amine surfactants was not able to be observed; however the excellent performance of composition 1-14 provides a strong hint of such interaction.

Example 2 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 2a. All are oil-in-water emulsions and were prepared by the process described in Example 1, except that for compositions 2-01 to 2-08 and 2-11 to 2-17 the lecithin was hydrated and dispersed not by ultrasonication but by microfluidizing, using a Model M-l 10F microfluidizer of Microfluidics International Corp., for 3 cycles.

Table 2a Conc. Glyphosate Weight % comp. g a. e./1 Lecithin Butyl Ethomeen MON CS-20 Plurafac stearate T/25 0818 A-38 2-01 220 0. 75 3. 75 3. 0 3. 0 2-02 220 0. 75 0. 75 3. 0 3. 0 2-03 220 0. 75 3. 75 3. 0 3. 0 2-04 220 0. 75 0. 75 3. 0 3. 0 2-OS 220 6. 00 1. 50 3. 0 3. 0 2-06 220 6. 00 1. 50 3. 0 3. 0 2-07 220 4. 00 1. 00 3. 0 3. 0 2-08 220 4. 00 1. 00 3. 0 3. 0 2-09 220 0. 75 3. 75 3. 0 3. 0 2-10 220 0. 75 0. 75 3. 0 3. 0 2-11 220 0. 75 3. 75 6. 0 2-12 220 0. 75 3. 75 6. 0 2-13 345 6. 00 1. 50 4. 5 4. 5 2-14 345 6. 00 1. 50 6. 0 3. 0 2-15 345 6. 00 1. 50 6. 0 6. 0 2-16 345 0. 50 7. 50 12. 0 6.001.504.54.53.02-17345

Glyphosate concentration in compositions 2-01 to 2-12 was approximately 20% a. e. by weight. Glyphosate concentration in compositions 2-13 to 2-17 was approximately 30% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 19 days after planting ABUTH and ECHCF, and evaluation of herbicidal effectiveness was done 15 days after application.

Accord and Roundup0 Ultra were diluted and applied as comparative treatments, as in Example 1. Results, averaged for all replicates of each treatment, are shown in Table 2b.

Table 2b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF Accord RO 150 45 82 250 55 71 350 80 72 450 88 77 1505583Roundup#Ultra 250 89 88 350 97 93 450 99 93 2-Ol 150 92 83 250 96 96 350 99 96 86450100 85932-02150 250 97 78 350 97 90 450 99 90 2-03 150 87 85 250 98 92 350 99 95 95450100 87892-04150 250 97 92 350 99 94 450 99 91 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 2-OS 150 87 77 250 98 89 350 99 93 450 99 84 2-06 150 12 18 250 96 73 350 99 85 450 99 84 2-07 150 82 89 250 88 96 350 96 98 450 97 97 2-08 150 88 94 250 95 90 350 99 98 450 99 98 2-09 150 94 94 250 95 100 350 97 99 450 99 98 2-10 150 94 94 250 98 99 350 99 97 450 99 96 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 2-11 150 83 81 250 94 88 350 98 93 450 99 99 2-12 150 68 79 250 95 96 350 98 100 450 99 98 2-13 150 86 98 250 95 98 350 99 100 450 100 98 2-14 150 85 98 250 98 98 350 99 98 450 100 98 2-15 150 86 95 250 97 97 350 99 95 450 100 96 2-16 150 93 94 250 98 98 350 99 98 450 100 97 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 2-17 150 95 96 250 98 100 350 100 100 450 100 98

Outstanding herbicidal effectiveness was provided by composition 2-11, containing 6% of the amine surfactant Ethomeen T/25. Composition 2-12, containing 6% of the alkylether surfactant CS-20, was slightly less effective at the lowest glyphosate rate on ABUTH.

Composition 2-03, containing 3% of each of Ethomeenz T/25 and CS-20, was at least as effective as composition 2-11. In all these compositions, lecithin content was 0.75% and butyl stearate content was 3.75%.

Herbicidal effectiveness elicited by the amine surfactant in this test was so high that a truly synergistic interaction between the alkylether and the amine surfactants was not able to be observed; however the excellent performance of composition 2-03 provides a strong hint of such interaction.

Example 3 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 3a. All were prepared by the following process.

Alkylether surfactant (CS-20 or Plurafac A-38) and amine surfactant (EthomeenTM T/25) were added to water in a formulating vessel and the resulting mixture was heated to 55°C for 2 hours in a shaker bath. The mixture was allowed to cool, then glyphosate IPA salt, in the form of MON 0139, was added with mild agitation to form a preliminary glyphosate/surfactant mixture. Lecithin (Avanti, 45% phospholipids) was then added to this preliminary mixture, with stirring to break up lumps. The mixture was left for about 1 hour to allow the lecithin to hydrate, then butyl stearate was added with further stirring. Stirring continued until no phase separation occurred. The mixture in the formulating vessel was then transferred to a microfluidizer (Microfluidics International Corp., Model M-l lOF) and microfluidized for 3-5 cycles at 10,000 psi (69 MPa). In each cycle, the vessel was rinsed with microfluidized mixture. In the last cycle, the finished composition was collected in a clean vessel.

Table 3a Conc. Glyphosate Weight % comp. g a. e./l Lecithin Butyl Ethomeen CS-20 Plurafac A-38stearateT/25 1.51.53.03.03-01220 3-02 220 1.5 1.5 3.0 3.0 3-03 220 1. 5 1. 5 6. 0 3. 0 3-04 220 1. 5 1. 5 6. 0 3. 0 3-05 220 3. 0 1. 5 3. 0 3. 0 3.01.53.03.03-06220 3-07 348 1. 5 1. 5 6. 0 3. 0 3-08 348 3. 0 1. 5 3. 0 3. 0

Glyphosate concentration in compositions 3-01 to 3-06 was approximately 20% a. e. by weight. Glyphosate concentration in compositions 3-07 and 3-08 was approximately 30% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 17 days after planting ABUTH and ECHCF, and evaluation of herbicidal effectiveness was done 18 days after application.

Accord and Roundup (t Ultra were diluted and applied as comparative treatments, as in Example 1. Results, averaged for all replicates of each treatment, are shown in Table 3b.

Table 3b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF Accord (t 100 28 32 200 41 37 300 73 64 400 22 30 Roundupt Ultra 100 38 32 200 82 73 300 89 91 400 97 89 51403-01100 200 89 75 300 96 92 400 95 98 3-02 100 76 57 200 98 81 300 97 86 400 96 98 3-03 100 69 60 200 98 63 300 95 82 400 99 90 3-04 100 61 60 200 94 84 300 97 89 400 99 97 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 3-OS 100 64 53 200 95 82 300 96 90 400 95 98 3-06 100 61 58 200 94 78 300 88 87 400 100 94 3-07 100 56 61 200 88 77 300 91 82 400 97 89 3-08 100 42 52 200 82 80 300 86 90 400 97 92

Compositions 3-01 to 3-08 of the invention exhibited a very high degree of herbicidal effectiveness on ABUTH and ECHCF, even by the high standard set by Roundup (t Ultra.

Example 4 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 4a. Brij 78 of ICI is polyoxyethylene stearylether having an average of 20 oxyethylene units per molecule. Brij 700 of ICI is polyoxyethylene stearylether having an average of 100 oxyethylene units per molecule. MON 0818 of Monsanto is a surfactant based on polyoxyethylene tallowamine having an average of 15 oxyethylene units per molecule.

Compositions 4-01 to 4-04 and 4-08 were prepared as follows. Glyphosate IPA salt in the form of MON 0139 was added in the desired amount to a weighed quantity of alkylether

and/or amine surfactant. Heat was applied to the alkylether surfactant to bring it into a flowable condition before adding MON 0139. The required amount of water was then added to bring the concentration of glyphosate and other ingredients to the desired level. The composition was finally subjected to high-shear mixing using a Silverson L4RT-A mixer fitted with a medium emulsor screen, operated for 3 minutes at 7,000 rpm.

Compositions 4-05 to 4-07 and 4-09 to 4-18 contained a colloidal particulate silica and were prepared as follows. The required amount of the selected silica was suspended in a concentrated glyphosate IPA salt solution (MON 0139) and agitated with cooling to ensure homogeneity of the resulting glyphosate/silica mixture. In the case of compositions 4-09 to 4-18 that also contained alkylether and amine surfactants, the selected surfactants were added in the required amount by weight. Heat was applied to the alkylether surfactant to bring it into a flowable condition before adding to the glyphosate/silica mixture. The required amount of water was then added to bring the concentration of glyphosate and other ingredients to the desired level. The composition was finally subjected to high-shear mixing using a Silverson L4RT-A mixer fitted with a medium emulsor screen, operated for 3 minutes at 7,000 rpm.

The following types of silica were used, all from Degussa: A = Aerosil 380; B = AerosilTM MOX-80; C = AerosilTM MOX-170.

All compositions of this Example except compositions 4-01 to 4-03 were acceptably storage-stable.

Table 4a Concentrate Glyphosate Weight % Type of composition g a. e./1 Brij 78 Brij 700 MON 0818 silica silica 4-01 488 3. 0 4-02 488 4. 5 4-03 488 6. 0 4-04 488 3. 0 4-OS 488 1. 5 A 4-06 488 1. 5 B+C (1: 1) 4-07 488 3. 0 A+B (1: 1) 4-08 488 1. 5 Concentrate Glyphosate Weight % Type of composition g a. e./1 Brij 78 Brij 700 MON 0818 silica silica 4-09 488 3. 0 3. 0 1. 5 A 4-10 488 4. 5 3. 0 1. 5 A 4-11 488 6. 0 3. 0 1. 5 A 4-12 488 3. 0 3. 0 1. 5 B+C (l : 1) 4-13 488 4. 5 3. 0 1. 5 B+C (1 : 1) 4-14 488 6. 0 3. 0 1. 5 B+C (1 : 1) 4-15 488 3. 0 3. 0 1. 5 A+B (1 : 1) 4-16 488 4. 5 3. 0 1. 5 A+B (1 : 1) 4-17 488 6. 0 3. 0 1. 5 A+B (1 : 1) 4-18 488 4. 5 3. 0 1. 5 B+C (1 : 1)

Glyphosate concentration in all compositions was approximately 40% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 16 days after planting ABUTH and ECHCF, and evaluation of herbicidal effectiveness was done 21 days after application.

Accord (R) and Roundup (R) Ultra were diluted and applied as comparative treatments, as in Example 1. Results, averaged for all replicates of each treatment, are shown in Table 4b.

Table 4b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF Accord ( 100 2 23 200 18 50 300 42 67 400 63 80 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 1002047Roundup#Ultra 200 40 86 300 83 98 400 93 98 10754-01100 200 62 83 300 80 96 400 93 99 4-02 100 40 60 200 77 92 300 87 97 9940093 23404-03100 200 38 63 300 78 91 9140097 4-04 100 20 38 7720023 200 23 77 9430043 400 73 94 7304-05100 4-05 100 7 30 200 25 37 300 42 60 6340067 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 4-06 100 7 30 200 20 53 300 52 67 400 83 67 4-07 100 5 35 200 20 63 300 57 80 400 43 85 4-08 100 22 83 200 47 99 300 86 98 400 78 100 4-09 100 12 45 200 25 77 300 40 83 400 37 95 4-10 100 13 53 200 73 99 300 85 98 400 99 99 4-11 100 25 50 200 60 88 9930093 400 99 99 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 4-12 100 25 45 200 57 88 300 85 97 400 100 94 4-13 100 30 52 200 68 87 300 93 99 400 100 92 4-14 100 40 45 200 73 88 300 81 98 400 100 99 4-15 100 8 57 200 33 96 300 81 99 400 95 99 4-16 100 10 62 200 48 83 300 99 98 400 100 100 4-17 100 27 58 200 65 92 300 75 98 400 93 99 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 4-18 100 5 40 200 33 87 300 55 98 400 75 98

Example 5 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 5a.

Compositions 5-12 to 5-14 were prepared in the same way as compositions 4-01 to 4-04 and 4-08 of Example 4. Compositions 5-01 to 5-11 and 5-15 to 5-17 were prepared in the same way as compositions 4-09 to 4-18 of Example 4. The following types of silica were used, all from Degussa: A = Aerosil 380; B = AerosilTM MOX-80; C = AerosilTM MOX-170.

Table 5a Conc. Glyphosate Weight % Type of comp. g a. e./l Brij 78 Ethomeen propylene silica silica T/25 glycol 5-01 488 3. 0 0. 8 A 6.01.5B+C(1:1)5-02488 5-03 488 4. 5 1. 5 A 5-04 488 4. 5 2. 25 0. 5 1. 5 A+B (2: 1) 5-05 488 4. 5 0. 5 1. 5 A+B (2: 1) 5-06 488 6. 0 0. 5 1. 5 A+B (2: 1) 5-07 488 3. 0 1. 50 0. 5 1. 5 A+B (2: 1) 5-08 488 6. 0 3. 00 0. 5 1. 5 A+B (2: 1) 3.01.500.51.5A5-09488 5-10 488 4. 5 2. 25 0. 5 1. 5 A 5-11 488 6. 0 3. 00 0. 5 1. 5 A 1.500.55-12488 Conc. Glyphosate Weight % Type of comp. g a. e./l Brij 78 Ethomeen propylene silica silica T/25 glycol 5-13 488 2. 25 0. 5 5-14 488 3. 00 0. 5 5-15 488 1. 50 0. 5 1. 5 A+B (2: 1) 5-16 488 2. 25 0. 5 1. 5 A+B (2: 1) 5-17 488 3. 00 0. 5 1. 5 A+B (2: 1)

Glyphosate concentration in all compositions was approximately 40% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 16 days after planting ABUTH and ECHCF, and evaluation of herbicidal effectiveness was done 20 days after application.

Accord and Roundup (R) Ultra were diluted and applied as comparative treatments, as in Example 1. Results, averaged for all replicates of each treatment, are shown in Table 5b.

Table 5b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF Accord (t 100 3 200 10 12 300 43 22 400 47 27 Roundup@Ultra 100 13 15 200 25 22 300 58 53 400 68 82 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 5-01 100 30 20 200 60 53 300 73 88 400 87 96 5-02 100 40 23 200 63 55 300 88 87 400 93 93 5-03 2042 200 72 55 300 82 83 400 90 88 5-04 100 60 32 200 70 57 300 90 88 400 90 93 5-05 100 47 32 200 67 57 300 88 85 400 94 88 5-06 3733 200 68 67 300 82 80 400 90 88 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 5-07 100 35 37 200 67 70 300 87 85 9340097 5-08 100 32 35 7720067 200 67 77 300 85 92 400 97 95 5-09 100 27 33 200 57 67 300 88 83 400 93 95 5-10 100 13 33 200 62 58 300 80 80 400 92 92 5-11 100 13 20 200 60 57 6330088 400 93 82 5-12 100 10 27 200 53 53 300 70 67 400 88 85 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 5-13 100 3 28 200 50 57 300 67 70 400 90 82 5-14 100 3 28 200 55 57 300 70 83 400 87 87 5-15 100 10 20 200 58 43 300 70 72 400 83 85 5-16 100 12 22 200 55 57 300 73 77 400 92 90 5-17 100 7 20 200 53 55 300 70 75 400 85 88

Example 6 Plant treatment compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 6a.

Compositions 6-01 to 6-08 are each representative of five plant treatment compositions prepared to provide glyphosate a. e. rates of 100,200,300,400 and 500 g a. e./ha when applied at 93 1/ha. Weight ratio of total surfactant to glyphosate a. e. was kept constant across all five glyphosate a. e. rates. Ratios shown in Table 6a are on an"active"surfactant basis, not an"as is"

surfactant basis, with respect to ArquadTM C-50, which is supplied at 50% concentration. The plant treatment compositions were prepared by simply mixing the ingredients in water at the required dilution. Glyphosate IPA salt was supplied as MON 0139. Plurafac A-38 of BASF is a polyoxyethylene C, 6, 8 alkylether surfactant having an average of about 27 oxyethylene units per molecule. Arquad C-50 of Akzo is a quaternary cocoalkyltrimethylammonium chloride surfactant having no oxyethylene units. MON 0818 of Monsanto is a surfactant based on polyoxyethylene tallowamine having an average of about 15 oxyethylene units per molecule.

Table 6a Composition Weight ratio of total : Surfactant (s) (weight ratio) surfactant/glyphosate a. e. 6-01 0. 4 : 1 Plurafac A-38 6-02 0. 4 : 1 Plurafac A-38 + Arquad C-50 (10: 1) 6-03 0. 4 : 1 Plurafac A-38 + Arquad C-50 (2: 1) 6-04 0. 4 : 1 Plurafac A-38 + Arquad C-50 (1: 1) 6-05 0. 4 : 1 Plurafac A-38 + Arquad C-50 (1: 2) 6-06 0. 4 : 1 Plurafac A-38 + Arquad C-50 (1: 10) 6-07 0. 4 : 1 Arquad C-50 6-08 0. 4 : 1 Plurafac A-38 + MON 0818 (1: 1)

Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 15 days after planting ABUTH and ECHCF, and evaluation of herbicidal effectiveness was done 19 days after application.

MON 0139 and Roundup@ Ultra were diluted and applied as comparative treatments.

Two sets of replicated pots were sprayed with Roundup0 Ultra, as the first and last set of treatments applied. Results, averaged for all replicates of each treatment, are shown in Table 6b.

Table 6b Plant treatment composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF MON0139 100 2 50 200 3 30 300 6 55 400 6 65 500 27 65 Roundup Ultra (first set) 100 12 0 200 6 52 300 47 65 400 72 70 500 83 73 Roundup# Ultra (second set) 100 4 38 200 5 42 300 30 63 400 60 78 500 68 81 1336-01100 572009 300 48 70 400 73 70 500 73 82 6-02 100 4 48 200 11 63 300 31 68 400 68 78 500 75 97 Plant treatment composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 6-03 100 0 23 200 5 57 300 9 68 400 62 82 500 67 79 51006-04100 200 17 63 300 16 68 400 67 68 500 72 77 6-05 100 5 27 200 7 53 300 47 63 400 63 73 500 73 80 4476-06100 200 6 53 300 20 62 400 50 68 500 70 78 6-07 100 7 48 200 4 52 300 7 63 400 15 88 500 48 80 Plant treatment composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 6-08 100 2 63 200 12 72 300 47 82 400 68 86 500 77 100

The synergistic interaction apparent from the data of Wyrill & Burnside, op. cit., between Plurafac A-46 and ArquadTM C-50, at very high surfactant to glyphosate a. e. ratios, was not evident in this test using Plurafac A-38 and Arquad C-50 at surfactant to glyphosate a. e. ratios in the realm of the present invention, which are much lower than those of Wyrill & Burnside.

Example 7 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 7a.

Compositions 7-01 to 7-05 were prepared by mixing the ingredients in water and agitating the mixture in a shaker bath at 50°C for 30 minutes. EthoquadTM C/25 of Akzo is a polyoxyethylene quaternary N-methyl cocoalkylammonium chloride surfactant having an average of about 15 oxyethylene units per molecule. EthoquadTM 18/25 of Akzo is a polyoxyethylene quaternary N-methyl stearylammonium chloride surfactant having an average of about 15 oxyethylene units per molecule.

Table 7a Concentrate Glyphosate Weight % a.e./lPlurafacA-38EthoquadC/25compositiong Ethoquad 18/25 3.07-0162 7-02 62 2.1 0.9 7-03 62 1. 5 1. 5 7-04 62 2. 1 0. 9 Concentrate Glyphosate Weight % composition g a. e./1 Plurafac A-38 Ethoquad C/25 Ethoquad 18/25 1.51.57-0562

Glyphosate concentration in all compositions was approximately 6% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above, except that 6 replicate pots of each species were subjected to each treatment, applied in two sets of three pots.

Evaluation of herbicidal effectiveness was done 14 days after application.

MON 0139 and Roundup0 Ultra were diluted and applied as comparative treatments.

Results, averaged for all replicates of each treatment, are shown in Table 7b.

Table 7b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF MON 0139 100 6 40 200 48 52 300 65 62 400 70 64 500 85 68 Roundup (g) Ultra 100 63 56 200 82 77 300 91 84 400 96 91 500 97 96 7-01 100 76 61 200 89 81 300 96 94 400 97 96 500 99 97 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 7-02 100 77 72 200 93 92 300 97 96 400 98 94 500 99 96 7-03 100 78 68 200 94 89 300 96 95 400 98 97 500 98 97 7-04 100 80 71 200 91 86 300 97 90 400 98 92 500 98 94 7-05 100 79 71 200 94 93 300 96 94 400 98 94 500 98 96

Compositions 7-02 to 7-05, each containing both an alkylether surfactant (Plurafac A- 38) and an amine surfactant (EthoquadTM C/25 or 18/25) as required by the present invention, exhibited greater herbicidal effectiveness in this test than composition 7-01, containing only the alkylether component but at the same total surfactant concentration.

Example 8 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 8a.

Compositions 8-01 to 8-06 were prepared by mixing the ingredients in water and agitating the mixture in a shaker bath at 50°C for 30 minutes. HetoxolTM CS-25 of Heterene is a polyoxyethylene C16-18 alkylether surfactant having an average of about 25 oxyethylene units per molecule. PlurafacTM A-38 of BASF is a polyoxyethylene C16-18 alkylether surfactant having an average of about 27 oxyethylene units per molecule. Ethomeen Tm T/25 of Akzo is a polyoxyethylene quaternary tallowamine surfactant having an average of about 15 oxyethylene units per molecule. Trymeen 6617 of Henkel is a polyoxyethylene quaternary tallowamine surfactant having an average of about 50 oxyethylene units per molecule.

Table 8a Conc. Glyphosate Weight % comp. g a. e./l Plurafac A-38 Hetoxol CS-25 Ethomeen T/25 Trymeen 6617 8-0l 62 3. 0 1.51.58-0262 8-03 62 3. 0 8-04 62 1. 5 1. 5 8-05 62 1. 5 1. 5 8-06 62 3. 0 Glyphosate concentration in all compositions was approximately 6% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) plants were grown and treated by the standard procedures given above, except that 6 replicate pots were subjected to each treatment, applied in two sets of three pots. Applications of plant treatment compositions were made 15 days after planting ABUTH, and evaluation of herbicidal effectiveness was done 14 days after application.

MON 0139 and Roundup0 Ultra were diluted and applied as comparative treatments.

Results, averaged for all replicates of each treatment, are shown in Table 8b.

Table 8b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH MON 0139 100 0 200 5 300 52 400 71 500 77 Roundup (g) Ultra 100 2 200 42 300 78 400 84 500 92 38-01100 200 52 30079 400 88 500 93 8-02 100 13 200 58 300 75 400 96 500 97 8-03 100 7 200 57 300 77 400 89 50094 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH 8-04 100 5 200 49 300 82 400 90 500 95 8-05 100 14 200 60 300 76 400 91 500 94 8-06 100 200 21 300 71 400 83 500 92

Example 9 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 9a.

Compositions 9-01 to 9-04 were prepared by mixing the ingredients in water and agitating the mixture in a shaker bath at 50°C for 30 minutes. Concentrations of Arquadz T-50, which is supplied at 50% concentration, are shown in Table 9a on an"active"surfactant basis, not an"as is"surfactant basis. Arquad T-50 of Akzo is a quaternary tallowalkyltrimethylammonium chloride surfactant having no oxyethylene units. EthoquadTM C/25 of Akzo is a polyoxyethylene quaternary N-methyl cocoalkylammonium chloride surfactant having an average of about 15 oxyethylene units per molecule. EthoquadTM 18/25 of Akzo is a polyoxyethylene quaternary N-methyl stearylammonium chloride surfactant having an average of about 15 oxyethylene units per molecule.

Table 9a Conc. Glyphosate Weight % comp. g a. e./I Plurafac A-38 Arquad T-50 Ethoquad C/25 Ethoquad 18/25 9-01 62 3. 0 9-02 62 1.5 1.5 9-03 62 1.5 1.5 9-06 62 1. 5 1. 5

Glyphosate concentration in all compositions was approximately 6% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) plants were grown and treated by the standard procedures given above, except that 6 replicate pots were subjected to each treatment, applied in two sets of three pots. Applications of plant treatment compositions were made 19 days after planting ABUTH, and evaluation of herbicidal effectiveness was done 14 days after application.

MON 0139 and Roundup Ultra were diluted and applied as comparative treatments.

Results, averaged for all replicates of each treatment, are shown in Table 9b.

Table 9b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH MON 0139 100 6 200 12 300 58 400 72 500 80 Roundupg Ultra 100 8 200 64 300 77 400 81 500 89 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH 9-01 100 57 200 76 30081 400 83 500 93 9-02 100 11 200 75 300 77 400 86 500 92 9-03 100 55 200 76 300 84 400 91 500 97 9-04 100 58 200 76 300 84 400 92 500 94

A mixture of the alkylether surfactant Plurafac A-38 with an amine surfactant having no oxyethylene units (Arquad T-50, composition 9-02) elicited significantly lower herbicidal effectiveness with glyphosate than a similar mixture with a polyoxyethylene amine surfactant (EthoquadTM C/25 or 18/25, compositions 9-03 and 9-04 respectively).

Example 10 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 1 osa.

Compositions 10-01 to 10-06 were prepared by mixing the ingredients in water and agitating the mixture in a shaker bath at 50°C for 30 minutes.

Table 10a Concentrate Glyphosate Weight % composition g a. e./l Hetoxol CS-25 Ethoquad 18/25 10-01 62 2.0 10-02 62 1. 6 0. 4 10-03 62 1. 4 0. 6 10-04 62 1. 0 1. 0 0.61.410-0562 2.010-0662

Glyphosate concentration in all compositions was approximately 6% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) plants were grown and treated by the standard procedures given above, except that 6 replicate pots were subjected to each treatment, applied in two sets of three pots. Applications of plant treatment compositions were made 23 days after planting ABUTH, and evaluation of herbicidal effectiveness was done 14 days after application.

MON 0139 and Roundup Ultra were diluted and applied as comparative treatments.

Results, averaged for all replicates of each treatment, are shown in Table 1 Ob.

Table 10b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH MON 0139 100 1 200 10 300 29 400 49 500 77 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH Roundup (t Ultra 100 10 200 60 300 82 400 86 500 93 10-01 100 42 200 78 300 88 400 95 500 98 10-02 100 53 200 83 30096 400 98 500 98 10-03 100 52 200 82 300 92 400 98 500 99 10-04 100 56 200 84 300 96 400 98 500 98 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH 10-05 100 49 200 82 300 96 400 97 500 98 10-06 100 7 200 55 300 75 400 92 500 98

Compositions 10-02 to 10-05 of the present invention, containing mixtures of an alkylether surfactant (HetoxolTM CS-25) and an amine surfactant (EthoquadTM 18/25) exhibited greater herbicidal effectiveness than either composition 10-01 (the alkylether alone) or 10-06 (the amine alone) at the same total surfactant concentration.

Example 11 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 11 a.

Compositions 11-01 to 11-04 were prepared by adding to water, in the following sequence, the alkylether surfactant Plurafac A-38, then soybean lecithin (95% phospholipids, Avanti), then the amine surfactant MON 0818, then butyl stearate. If a coupling agent was included, it was added first to the Plurafac A-38 before other ingredients were added. The resulting mixture was fan mixed for 10 minutes and then placed in a shaker bath at 50°C for 30 minutes. Finally glyphosate IPA salt in the form of MON 0139 was added and the composition thoroughly mixed.

Table lla Conc. Glyphosate Weight % Coupling comp. g a. e./l Lecithin Butyl Plurafac MON Coupling agent stearate A-38 0818 agent 11-01 220 3. 0 1. 5 3. 0 3. 0 none 11-02 357 5. 0 2. 5 4. 5 4. 5 2. 5 ethanol 11-03 357 5. 0 2. 5 4. 5 4. 5 1. 0 urea 5.02.54.54.51.0N(Bu)4OH*11-04347 * tetrabutylammonium hydroxide Glyphosate concentration in composition 11-01 was approximately 20% a. e. by weight, in compositions 11-02 to 11-04 was approximately 30% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) plants were grown and treated by the standard procedures given above, except that 6 replicate pots were subjected to each treatment, applied in two sets of three pots. Applications of plant treatment compositions were made 17 days after planting ABUTH, and evaluation of herbicidal effectiveness was done 14 days after application.

MON 0139 and Roundup Ultra were diluted and applied as comparative treatments.

Results, averaged for all replicates of each treatment, are shown in Table 11b.

Table 11b

Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH MON 0139 100 22 200 38 400 74 600 89 800 92 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH Roundup Ultra 100 46 200 69 400 96 600 98 800 100 6611-01100 200 77 400 96 600 98 800 100 11-02 100 62 200 77 400 97 600 99 800 100 11-03 100 65 200 77 400 96 600 99 800 99 11-04 100 58 200 78 400 97 600 97 800 100

Example 12 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 12a.

Compositions 12-01 to 12-03 were prepared by the following procedure. Soybean lecithin (95% phospholipids, Avanti) was added to water and fan-mixed with a Variac mixer, set at 30% of maximum voltage, for about 10 minutes, to hydrate the lecithin. To the hydrated lecithin in water were then added the alkylether surfactant Plurafac A-38, the amine surfactant MON 0818, butyl stearate, a coupling agent and glyphosate IPA salt in the form of MON 0139.

The resulting mixture was first agitated by hand shaking and then mixed with a Turrax mixer at 20,000 rpm for about 8 minutes.

Table 12a Conc. Glyphosate Weight % Coupling comp. g a. e./l Lecithin Butyl Plurafac MON Coupling agent stearate A-38 0818 agent 12-01 354 5. 0 2. 5 4. 5 4. 5 0. 5 DMSO 12-02 330 4. 0 2. 0 6. 5 6. 0 1. 0 butanol 4.02.08.54.51.0butanol12-03353 Glyphosate concentration in each composition was approximately 30% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) plants were grown and treated by the standard procedures given above, except that 6 replicate pots were subjected to each treatment, applied in two sets of three pots. One set of three pots for each treatment was subjected to simulated rain, using an overhead irrigator, in the amount of 6 mm, one hour after application of plant treatment compositions. Applications of plant treatment compositions were made 18 days after planting ABUTH, and evaluation of herbicidal effectiveness was done 14 days after application.

MON 0139 and Roundup@ Ultra were diluted and applied as comparative treatments.

Results, averaged for all replicates of each treatment, are shown in Table 12b.

Table 12b Concentrate composition Glyphosate rate % Inhibition, ABUTH g a. e./ha no rain rain MON 0139 250 53 27 500 82 42 750 96 62 Roundup (g) Ultra 250 88 47 500 99 70 750 100 87 12-01 250 97 66 500 99 83 750 100 95 12-02 250 97 77 500 99 90 750 100 97 12-03 250 95 73 500 100 86 750 100 96

Compositions 12-01 to 12-03 are representative of an embodiment of the present invention in which an alkylether and an amine surfactant are coformulated with glyphosate in such a way that the glyphosate is believed strongly associated with or entrapped by supramolecular aggregates. Compositions 12-01 to 12-03 in the greenhouse test of this Example not only exhibited significantly enhanced herbicidal effectiveness over that provided by the commercial standard Roundup Ultra in the absence of rain; in addition, they showed greatly enhanced rainfastness.

Example 13 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 13a.

Composition 13-01 contained a colloidal particulate silica, Aerosil 380 of Degussa, and was prepared as follows. The required amount of Aerosil 380 was suspended in a concentrated glyphosate IPA salt solution (MON 0139) and agitated with cooling to ensure homogeneity of the resulting glyphosate/silica mixture. The alkylether surfactant Plurafac A- 38 was heated to bring it into a flowable condition and was then added in the required amount by weight to the glyphosate/silica mixture. The required amount of water was then added to bring the concentration of glyphosate and other ingredients to the desired level. The composition was finally subjected to high-shear mixing using a Silverson L4RT-A mixer fitted with a medium emulsor screen, operated for 3 minutes at 7,000 rpm. Compositions 13-02 to 13-05 were prepared by the procedure of Example 12.

Table 13a Conc. Glyphosate Weight % Other comp. g a. e./l Lecithin Butyl Plurafac MON Other ingredient stearate A-38 0818 13-01 357 10. 0 1. 25 Aerosil 380 13-02 347 4. 0 2. 0 6. 5 6. 5 1. 0 butanol 13-03 349 4. 0 2. 0 8. 5 4. 5 1. 0 butanol 13-04 335 4. 0 2. 0 10. 0 3. 0 1. 0 butanol 13-05 357 5. 0 2. 0 4. 5 4. 5 1. 0 DMSO Glyphosate concentration in each composition was approximately 30% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) plants were grown and treated by the standard procedures given above, except that 6 replicate pots were subjected to each treatment, applied in two sets of three pots. One set of three pots for each treatment was subjected to simulated rain, using an overhead irrigator, in the amount of 6 mm, one hour after application of plant treatment compositions. Applications of plant treatment compositions were made 22 days after planting ABUTH, and evaluation of herbicidal effectiveness was done 14 days after application.

MON 0139 and Roundup0 Ultra were diluted and applied as comparative treatments.

Results, averaged for all replicates of each treatment, are shown in Table 13b.

Table 13b Concentrate composition Glyphosate rate % Inhibition, ABUTH g a. e./ha no rain rain MON 0139 300 68 10 600 93 35 900 97 60 Roundup@Ultra 300 78 57 600 98 70 900 100 73 13-01 300 98 70 600 99 86 900 100 98 13-02 300 97 67 600 99 93 900 100 93 13-03 300 98 72 600 100 82 900 100 97 13-04 300 98 70 600 99 85 900 100 93 13-05 300 100 68 600 98 94 900 100 98

Compositions 13-02 to 13-05 are representative of an embodiment of the present invention in which an alkylether and an amine surfactant are coformulated with glyphosate in such a way that the glyphosate is believed strongly associated with or entrapped by supramolecular aggregates. Compositions 13-02 to 13-05 in the greenhouse test of this Example not only exhibited significantly enhanced herbicidal effectiveness over that provided by the

commercial standard Roundup# Ultra in the absence of rain; in addition, they showed greatly enhanced rainfastness. Excellent herbicidal effectiveness and rainfastness were also exhibited by composition 13-01, which contains an alkylether surfactant but no amine surfactant.

Example 14 Aqueous concentrate compositions were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 14a.

Composition 14-01 was prepared by adding colloidal particulate silica (a blend of Aerosil 380 and AerosilTM MOX-80 of Degussa, in a 9: 1 weight ratio) to glyphosate IPA salt solution (MON 0139) and mixing with a Turrax mixer on ice at 20,500 rpm for about 8 minutes to form a glyphosate/silica mixture. The alkylether surfactant PlurafacT A-38 of BASF was then added to the glyphosate/silica mixture and the resulting composition was mixed with a Turrax mixer on ice at 20,500 rpm for a further 5 minutes. Compositions 14-02 to 14-05 were prepared by the procedure of Example 12.

Table 14a Conc. Glyphosate Weight % Other comp. g a. e./l Lecithin Butyl Plurafac MON Other ingredient stearate A-38 0818 13.52.5colloidalsilica14-01472 14-02 351 5. 0 2. 5 4. 5 4. 5 0. 5 DMSO 14-03 354 6. 0 2. 5 6. 5 6. 5 0. 5 DMSO 14-04 335 5. 0 2. 5 6. 5 4. 5' 0. 5 butanol 5.02.56.528.51.0urea14-05357 11MON 0818 replaced in this composition by EthoquadTM 18/25 Akzo 2PlurafacTM A-38 replaced in this composition by HetoxolTM CS-25 of Heterene Glyphosate concentration in composition 14-01 was approximately 40% a. e. by weight.

Glyphosate concentration in compositions 14-02 to 14-05 was approximately 30% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above, except that 6 replicate pots of each species were subjected to each treatment, applied in two sets of three pots.

Applications of plant treatment compositions were made 18 days after planting ABUTH and ECHCF, and evaluation of herbicidal effectiveness was done 15 days after application.

MON 0139 and Roundup Ultra were diluted and applied as comparative treatments.

Results, averaged for each treatment, are shown in Table 14b.

Table 14b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF MON0139 100 1 4 200532 300 45 45 400 56 50 500 59 Roundup (t Ultra 100 31 44 200 74 56 300 88 72 400 97 77 500 99 86 14-01 100 53 43 200 74 48 300 88 64 400 94 78 500 99 86 14-02 100 45 40 200 73 52 300 92 65 400 97 77 500 99 89 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 14-03 100 55 37 200 79 58 300 94 72 400 95 74 500 99 94 14-04 100 57 41 200 81 61 300 93 76 400 96 80 500 99 88 14-05 100 57 43 200 81 60 300 94 79 400 98 82 500 99 91

Example 15 Aqueous concentrate compositions 15-01 to 15-05 were prepared identically to compositions 14-01 to 14-05 respectively, as shown in Table 15a.

Table 15a Conc. Glyphosate Weight % Other comp. g a. e./l Lecithin Butyl Plurafac MON Other ingredient stearate A-38 0818 15-01 472 13. 5 2. 5 colloidal silica 15-02 351 5. 0 2. 5 4. 5 4. 5 0. 5 DMSO 15-03 354 6. 0 2. 5 6. 5 6. 5 0. 5 DMSO 15-04 335 5. 0 2. 5 6. 5 4. 5 0. 5 butanol Conc. Glyphosate Weight % Other comp. g a. e./l Lecithin Butyl Plurafac MON Other ingredient stearate A-38 0818 5.02.56.528.51.0urea15-05357 'MON 0818 replaced in this composition by EthoquadTM 18/25 of Akzo 2 Plurafac A-38 replaced in this composition by HetoxolTM CS-25 of Heterene Velvetleaf (Abutilon theophrasti, ABUTH), morningglory (Ipomoea sp., IPOSS) and prickly sida (Sida spinosa, SIDSP) plants were grown and treated by the standard procedures given above, except that 6 replicate pots of each species were subjected to each treatment, applied in two sets of three pots. Applications of plant treatment compositions were made 13 days after planting IPOSS, 20 days after planting ABUTH and 24 days after planting SIDSP, and evaluation of herbicidal effectiveness was done 15 days after application.

MON 0139 and Roundup@ Ultra were diluted and applied as comparative treatments.

Results, averaged for each treatment, are shown in Table 15b.

Table 15b

Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH IPOSS SIDSP MON 0139 100 1 0 13 200 29 17 30 400 62 63 58 600 70 68 68 800 81 79 73 Roundup (g) Ultra 100 46 45 200 75 60 68 400 90 75 84 600 98 84 83 800 99 94 92 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH IPOSS SIDSP 15-01 100 71 11 8 200 81 46 78 400 93 71 84 600 98 82 90 800 99 91 88 15-02 100 61 5 48 200 81 67 66 400 94 81 82 600 97 91 88 800 98 94 91 15-03 100 66 8 51 200 83 59 69 400 94 75 84 600 98 85 90 800 99 92 93 15-04 100 71 20 57 200 83 69 77 400 96 81 85 600 98 92 92 800 99 98 94 15-05 100 68 17 68 200 84 54 78 400 97 81 86 600 98 88 92 800 99 95 94

Example 16 Solid water-soluble granule compositions were prepared containing glyphosate ammonium salt and excipient ingredients as shown in Table 16a.

Compositions 16-01 to 16-13 were all prepared by the following procedure. The selected surfactant or surfactants were first heated if necessary to bring them to a flowable state. Dry ammonium glyphosate powder (MON 8750 of Monsanto) was mixed with a small amount of water (typically about 5 g per 100 g of all other ingredients) and with the selected surfactant (s) to make a wet mix which was kneaded until a homogeneous smooth dough-like paste was produced. This paste was transferred to a radial extruder fitted with screens having 1 mm orifices and extruded through these orifices. The resulting strands of extrudate broke spontaneously to form short cylindrical granules which were then dried in a fluid bed dryer.

Surfactants used in the compositions of this Example were the alkylether surfactant Plurafac A-38 of BASF and amine surfactants A, B and C, respectively polyoxyethylene (20) tallowamine, polyoxyethylene (10) N-methyl tallowammonium chloride (Ethoquad T/20 of Akzo), and a surfactant of formula where R'is C12-15 alkyl, y is 2 and x'+ x"is an average number of about 5, as disclosed in U. S.

Patent No. 5,750,468.

Table 16a Granule Weight % composition Glyphosate Plurafac amine amine amine a. e. A-38 surfactant A surfactant B surfactant C 16-01 68. 0 21. 4 0 16-02 68. 0 16. 0 5. 4 16-03 68. 0 10. 7 10. 7 16-04 68. 0 5. 5 16. 0 16-OS 68. 0 21. 4 Granule Weight % composition Glyphosate Plurafac amine amine amine a. e. A-3 8 surfactant A surfactant B surfactant C 16-06 68. 0 16. 0 5. 4 16-07 68. 0 10. 7 10. 7 16-08 68. 0 5. 5 16. 0 16-09 68. 0 21. 4 16-10 68. 0 16. 0 5. 4 16-11 68. 0 10. 7 10. 7 16-12 68. 0 5. 5 16. 0 16-13 68. 0 21. 4

Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 17 days after planting ABUTH and ECHCF, and evaluation of herbicidal effectiveness was done 20 days after application.

Roundup Ultra was diluted and applied as a comparative treatment. Results, averaged for all replicates of each treatment, are shown in Table 16b.

Table 16b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF Roundup (M Ultra 50 3 10 100 50 53 200 85 88 300 90 100 500 100 97 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 33516-0150 100 70 53 200 93 83 300 98 92 500 100 100 16-02 50 30 13 100 70 67 200 93 87 96300100 99500100 232716-0350 100 68 70 200 98 89 300 99 95 500 100 99 16-04 50 10 33 100 67 73 200 98 93 300 99 99 500 100 98 16-05 50 0 20 100 33 57 200 87 82 300 95 93 500 100 97 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 16-06 50 42 28 100 72 60 200 94 83 300 100 95 500 100 98 16-07 50 38 45 100 75 70 200 99 85 300 100 99 500 100 100 16-08 50 30 47 100 75 70 200 99 85 300 99 99 500 100 94 16-09 50 0 17 100 33 67 200 87 83 300 98 94 500 100 92 16-10 50 32 40 100 70 57 200 94 83 300 100 95 500 100 100 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 16-11 50 17 43 100 67 65 200 97 97 300 100 97 500 100 98 16-12 50 10 30 100 60 65 200 88 85 300 98 90 500 100 95 16-13 50 20 100 10 55 200 65 83 300 95 83 500 100 100

Clear evidence of a synergistic interaction between the alkylether surfactant and the amine surfactant component of the compositions of this Example was seen. In the case of amine surfactants A and C, the synergism was evident on ECHCF; in the case of amine surfactant B, the synergism was evident on both ABUTH and ECHCF.

Example 17 Solid water-soluble granule compositions were prepared containing glyphosate ammonium salt and excipient ingredients as shown in Table 17a.

Compositions 17-01 to 17-10 were prepared by the procedure described in Example 16.

Compositions 17-06 to 17-10 contained, in addition to glyphosate ammonium salt and surfactant (s), ammonium sulfate. This was added to the wet mix during kneading.

Table 17a Granule Weight % composition Glyphosate Plurafac amine ammonium a. e. A-38 surfactant A sulfate 17-01 68. 0 21. 0 17-02 68. 0 15. 8 5. 2 17-03 68. 0 10. 5 10. 5 17-04 68. 0 5. 2 15. 8 17-05 68.0 21. 0 17-06 34. 0 10. 3 50. 0 17-07 34. 0 7. 8 2. 5 50. 0 17-08 34. 0 5. 2 5. 2 50. 0 17-09 34.0 2.5 7.8 50.0 17-10 34. 0 10. 3 50. 0

Filaree (Erodium sp., EROSS) and annual bluegrass (Poa annua, POAAN) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 41 days after planting EROSS and POAAN, and evaluation of herbicidal effectiveness was done 21 days after application.

Roundup Ultra was diluted and applied as a comparative treatment. Results, averaged for all replicates of each treatment, are shown in Table 17b.

Table 17b Concentrate composition Glyphosate rate % Inhibition g a. e./ha EROSS POAAN Roundup Ultra 400 50 30 600 75 43 800 88 60 1000 97 75 Concentrate composition Glyphosate rate % Inhibition g a. e./ha EROSS POAAN 774017-01400 600 87 63 800 88 68 1000 95 70 17-02 400 70 22 600 85 42 800 95 53 1000 90 75 17-03 400 78 30 600 90 45 800 93 65 1000 95 68 17-04 400 72 37 600 88 53 800 93 53 1000 93 67 17-05 400 52 28 600 67 42 800 83 42 1000 95 63 17-06 400 68 38 600 90 45 800 92 48 1000 97 62 Concentrate composition Glyphosate rate % Inhibition g a. e./ha EROSS POAAN 17-07 400 80 40 600 88 45 800 97 62 1000 95 68 17-08 400 82 48 600 92 63 800 98 60 1000 97 78 17-09 400 73 53 600 88 60 800 90 63 1000 97 88 17-10 400 55 37 600 80 48 800 85 48 1000 92 62

A synergistic interaction between the alkylether surfactant and the amine surfactant component of the compositions of this Example was evident on EPOSS and POAAN for compositions 17-07 to 17-09 containing ammonium sulfate.

Example 18 Solid water-soluble granule compositions were prepared containing glyphosate ammonium salt and excipient ingredients as shown in Table 18a.

Compositions 18-01 to 18-05 were prepared by the procedure described in Example 16 and were identical to compositions 17-01 to 17-05 respectively.

Table 18a Granule Weight % composition Glyphosate Plurafac amine ammonium a.e. A-38 surfactant A sulfate 21.018-0168.0 18-02 68.0 15.8 5.2 10.510.518-0368.0 5.215.818-0468.0 18-OS 68.0 21.0

Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 18 days after planting ABUTH and ECHCF, and evaluation of herbicidal effectiveness was done 17 days after application.

Roundup (t Ultra was diluted and applied as a comparative treatment. Results, averaged for all replicates of each treatment, are shown in Table 18b.

Table 18b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF Roundupg Ultra 100 18 35 200 83 70 300 90 82 400 98 90 600 98 90 18-01 100 53 35 200 85 73 300 97 85 400 100 94 600 100 98 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 18-02 100 68 47 200 94 77 300 99 87 400 100 95 600 100 99 18-03 100 60 50 200 95 80 300 97 93 400 100 95 600 100 98 18-04 100 40 50 200 85 78 300 98 90 400 99 95 600 100 99 18-05 100 10 27 200 78 58 300 83 77 400 90 87 600 99 93

A synergistic interaction between the alkylether surfactant and the amine surfactant component of the compositions of this Example was evident on ABUTH and ECHCF.

Example 19 Solid water-soluble granule compositions were prepared containing glyphosate ammonium salt and excipient ingredients as shown in Table 19a.

Compositions 19-01 to 19-10 were prepared by the procedure described in Example 16.

Compositions 19-06 to 19-10 contained, in addition to glyphosate ammonium salt and surfactant (s), ammonium sulfate. This was added to the wet mix during kneading.

Compositions 19-01 to 19-10 were identical to compositions 17-01 to 17-10 respectively.

Table 19a Granule Weight % composition Glyphosate Plurafac amine ammonium a. e. A-38 surfactant A sulfate 19-01 68. 0 21. 0 19-02 68. 0 15. 8 5. 2 10.510.519-0368.0 5.215.819-0468.0 19-OS 68.0 21.0 19-06 34. 0 10. 3 50. 0 19-07 34. 0 7. 8 2. 5 50. 0 19-08 34. 0 5. 2 5. 2 50. 0 19-09 34. 0 2. 5 7. 8 50. 0 19-10 34. 0 10. 3 50. 0

Indian mustard (Brassica juncea, BRSJU) and downy brome (Bromus tectorum, BROTE) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 25 days after planting BRSJU and BROTE, and evaluation of herbicidal effectiveness was done 18 days after application.

Roundup Ultra was diluted and applied as a comparative treatment. Results, averaged for all replicates of each treatment, are shown in Table 19b.

Table 19b Concentrate composition Glyphosate rate % Inhibition g a. e./ha BRSJU BROTE 3004890Roundup#Ultra 400 62 93 600 65 97 800 88 99 19-01 300 42 52 400 60 67 600 80 85 800 83 90 19-02 300 43 53 400 75 88 600 77 98 800 88 99 19-03 300 50 82 400 82 93 9760090 800 92 100 528019-04300 9440075 600 85 98 800 92 100 526319-05300 400 68 90 600 87 98 800 87 98 Concentrate composition Glyphosate rate % Inhibition g a. e./ha BRSJU BROTE 19-06 300 57 65 400 58 85 600 78 93 800 85 99 19-07 300 47 57 400 73 83 600 83 97 800 90 100 19-08 300 52 88 400 82 94 600 85 98 800 92 99 19-09 300 62 80 400 78 87 600 92 99 800 90 100 19-10 300 47 60 400 63 87 600 83 97 800 92 99

A synergistic interaction between the alkylether surfactant and the amine surfactant component of the compositions of this Example was evident on BRSJU and BROTE.

Example 20 Solid water-soluble granule compositions were prepared containing glyphosate ammonium salt and excipient ingredients as shown in Table 20a.

Compositions 20-01 to 20-04 were prepared by the procedure described in Example 16.

These compositions contained, in addition to glyphosate ammonium salt and surfactant (s),

polyethylene glycol having an average molecular weight of about 8000 (PEG 8000). This was heated to a flowable state and added to the wet mix during kneading. Surfactants used in the compositions of this Example were the following alkylether surfactants: polyoxyethylene stearylether having an average of about 10 oxyethylene units per molecule (steareth-10, BrijTM 76 of ICI), and polyoxyethylene stearylether having an average of about 20 oxyethylene units per molecule (steareth-20, Emthox 5888 of Henkel). Compositions included for comparative purposes contained other nonionic surfactants: polyoxyethylene dodecylphenol having an average of about 10 oxyethylene units per molecule (POE (10) dodecylphenol), or polyoxyethylene sorbitan laurylester having an average of about 20 oxyethylene units per molecule (Tween 20 of ICI). The amine surfactant in all compositions was either polyoxyethylene N-methyl tallowammmonium chloride having an average of about 15 oxyethylene units per molecule (Ethoquad T/25 of Akzo) of polyoxyethylene N-methyl stearylammonium chloride having an average of about 15 oxyethylene units per molecule (EthoquadTM 18/25 of Akzo). It will be recognized that these two amine surfactants are very similar, differing only in the hydrogenation of the tallowalkyl group to provide a stearyl moiety in EthoquadTM 18/25.

Table 20a Granule Weight % Nonionic composition Glyphosate nonionic Ethoquad PEG surfactant a. e. surfactant T/25 8000 20-01 20.010.0Tween10.0 20 10.020.0110.0POE(10)20-0264.5 dodecylphenol 20-03 64.5 10. 0 20. 0 10. 0 steareth-10 20-04 64. 5 10. 0 20. 0 10. 0 steareth-20 'EthoquadTM 18/25 in place of Ethoquad T/25 Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 18 days after planting ABUTH and ECHCF, and evaluation of herbicidal effectiveness was done 17 days after application.

Roundup Ultra was diluted and applied as a comparative treatment. Also included as a comparative treatment was composition 16-05 of Example 16. Results, averaged for all replicates of each treatment, are shown in Table 20b.

Table 20b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF Roundup (g) Ultra 100 20 30 200 78 65 300 97 72 500 100 96 53016-05100 200 63 63 300 85 70 500 100 77 20-01 100 2 43 200 72 67 300 96 73 500 100 82 20-02 100 5 13 200 67 68 300 85 75 500 99 77 20-03 100 12 40 200 88 67 300 96 73 500 99 92 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 20-04 100 75 45 200 98 73 300 99 77 500 100 90

Compositions 20-03 and 20-04 of the invention exhibited greater herbicidal effectiveness than compositions 20-01 or 20-02, especially on ABUTH. The superior herbicidal effectiveness of composition 20-04, containing the alkylether surfactant steareth-20 and the amine surfactant Ethoquad T/25, was particularly marked.

Example 21 Solid water-soluble granule compositions were prepared containing glyphosate ammonium salt and excipient ingredients as shown in Table 21 a.

Compositions 21-01,21-03 and 21-04 were prepared by the procedure described in Example 16 and were identical to compositions 20-01,20-03 and 20-04 respectively.

Table 21a Granule Weight % Nonionic composition Glyphosate nonionic Ethoquad PEG surfactant a. e. surfactant T/25 8000 21-01 64. 5 10. 0 20. 0 10. 0 Tween 20 21-03 64. 5 10. 0 20. 0 10. 0 steareth-10 21-04 64. 5 10. 0 20. 0 10. 0 steareth-20

Kochia (Kochia scoparia, KCHSC), downy brome (Bromus tectorum, BROTE) and winter wheat (Triticum aestivum, TRZAW) plants were grown and treated by the standard procedures given above, except that six replicate pots of KCHSC were subjected to each treatment. Applications of plant treatment compositions were made 35 days after planting KCHSC, 27 days after planting BROTE and 14 days after planting TRZAW. Evaluation of

herbicidal effectiveness was done 15 days after application on KCHSC and 21 days after application on BROTE and TRZAW.

Roundup@ Ultra was diluted and applied as a comparative treatment. Also included as a comparative treatment was composition 16-05 of Example 16. Results, averaged for all replicates of each treatment, are shown in Table 21b.

Table 21b Concentrate composition Glyphosate rate % Inhibition g a. e./ha KCHSC BROTE TRZAW Roundup Ultra 100 37 46 33 200 83 86 76 400 99 92 97 600 100 99 97 16-05 100 36 12 50 200 82 63 81 400 98 82 99 600 100 100 100 21-01 100 35 28 59 200 84 74 87 400 99 92 100 60010098100 21-03 100 43 36 56 200 77 90 94 400 96 99 98 600 100 100 99 21-04 100 44 43 37 200 92 73 91 400 100 99 100 600 100 100 100

Example 22 Solid water-soluble granule compositions were prepared containing glyphosate ammonium salt and excipient ingredients as shown in Table 22a.

Compositions 22-01 to 22-17 were prepared by the procedure described in Example 16.

All compositions contained amine surfactant A of Example 16. Alkylether surfactants used in the compositions of this Example were polyoxyethylene (C, 2 alkyl) ether having an average of about 4 oxyethylene units per molecule (laureth-4, Brij 30 of ICI), polyoxyethylene (C12 alkyl) ether having an average of about 12 oxyethylene units per molecule (laureth-12, TrycolTM 5967 of Henkel), polyoxyethylene (Cl2 alkyl) ether having an average of about 23 oxyethylene units per molecule (laureth-23, Trycol 5964 of Henkel), polyoxyethylene (Cl8 alkyl) ether having an average of about 5 oxyethylene units per molecule (steareth-5), polyoxyethylene (Cl8 alkyl) ether having an average of about 10 oxyethylene units per molecule (steareth-12, Brij 76 of ICI), polyoxyethylene (C ; s alkyl) ether having an average of about 20 oxyethylene units per molecule (steareth-20, EmthoXTm 5888 of Henkel), polyoxyethylene (C18 alkyl) ether having an average of about 30 oxyethylene units per molecule (steareth-30, STA-30 of Heterene), and polyoxyethylene (Cl6 l8 alkyl) ether having an average of about 23 oxyethylene units per molecule (ceteareth-23, Plurafac A-38 of BASF).

Table 22a Granule Weight % Alkylether composition Glyphosate alkylether amine surfactant a. e. surfactant surfactant A 22-01 68. 0 21. 4 laureth-4 22-02 68. 0 10. 7 10. 7 laureth-4 22-03 68. 0 21. 4 laureth-12 22-04 68. 0 10. 7 10. 7 laureth-12 22-05 68. 0 21. 4 laureth-23 22-06 68. 0 10. 7 10. 7 laureth-23 22-07 68. 0 21. 4 steareth-5 22-08 68. 0 10. 7 10. 7 steareth-5 22-09 68. 0 21. 4 steareth-10 Granule Weight % Alkylether composition Glyphosate alkylether amine surfactant a. e. surfactant surfactant A 22-10 68. 0 10. 7 10. 7 steareth-10 22-11 68. 0 21. 4 steareth-20 22-12 68. 0 10. 7 10. 7 steareth-20 22-13 68. 0 21. 4 steareth-30 22-14 68. 0 10. 7 10. 7 steareth-30 22-15 68. 0 21. 4 ceteareth-23 22-16 68. 0 10. 7 10. 7 ceteareth-23 22-17 68. 0 21. 4 none

Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crits-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 17 days after planting ABUTH and ECHCF, and evaluation of herbicidal effectiveness was done 15 days after application.

Roundup Ultra was diluted and applied as a comparative treatment. Results, averaged for all replicates of each treatment, are shown in Table 22b. Where a blend of alkylether and amine surfactants elicited herbicidal effectiveness that was at least equal to the better of the alkylether or amine surfactant alone at the same total surfactant concentration, the average percent inhibition nn for that blend is highlighted thus: ** nn **.

Table 22b Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF RoundupW Ultra 100 50 28 200 85 40 300 99 57 500 100 89 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 0322-01100 200 10 8 300 70 33 500 80 45 0**37**22-02100 200 63 22 300 80 55 500 90 90 03022-03100 200 60 38 300 82 53 9050090 **27****33**22-04100 200 78 40 300 ** 92 ** 55 **93**50098 53322-05100 200 73 43 300 90 57 500 95 92 22-06 100 2 35 200 73 42 **73**30087 **100**50094 Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 22-07 100 0 8 200 8 27 300 32 43 500 55 47 3**37**22-08100 200 85 48 300 96 70 99****95**500** 22-09 100 5 20 200 78 32 300 88 47 500 92 97 22-10 55****38**** 83****47**200** 93****73**300** 99****100**500** 22-11 100 53 35 200 87 47 300 96 75 500 99 98 22-12 78****35**** 93**45200** 98****87**300** 100**97500** Concentrate composition Glyphosate rate % Inhibition g a. e./ha ABUTH ECHCF 22-13 100 73 30 200 87 38 300 97 57 500 99 95 **77****33**22-14100 200 ** 87 ** ** 47 ** 300300** **62**** 300 97 62 500 ****95**99 22-15 100 75 28 200 89 45 300 98 77 500 100 95 22-16 100 77 35 200 88 47 300 98 88 **99**50099 22-17 100 13 27 200 80 42 300 83 60 500 99 73

It will be noted from Table 22b that where the alkylether surfactant had a relatively short- chain (C12) alkyl moiety, as in compositions 22-01 to 22-06, some sporadic instances were observed of the blend with amine surfactant A eliciting herbicidal effectiveness at least equal to the better of the alkylether or the amine. With longer-chain (C16-18) alkylethers, however, equal to superior performance of the blend with amine surfactant A (by comparison with either surfactant alone) was the rule rather than the exception. In some instances there was evidence of

a very dramatic synergistic interaction. The greatest degree of herbicidal effectiveness was obtained with C) 6-) 8 alkylethers having 20 or more oxyethylene units per molecule.

Example 23 Aqueous concentrate compositions 23-01 and 23-02 were prepared containing glyphosate IPA salt and excipient ingredients as shown in Table 23a. Neodol 25-9 of Shell is a polyoxyethylene C13 alkylether surfactant having an average of about 9 oxyethylene units per molecule. Brij 56 of ICI is a polyoxyethylene cetylether surfactant having an average of about 10 oxyethylene units per molecule. Rhodaquat DAET of Rhodia is a quaternary ditallowalkylammonium sulfate surfactant having no oxyethylene units. Ethoquad T/25 of Akzo is a polyoxyethylene quaternary tallowalkyltrimethylammonium chloride surfactant having an average of about 15 oxyethylene units per molecule. Compositions 23-01 and 23-02 were prepared by mixing the ingredients with heating and agitation until the resulting mixture was homogeneous. Composition 23-01 was included for comparative purposes only.

Table 23a Concentrate Glyphosate Weight % composition g a. e./l Neodol 25-9 Brij 56 Rhodaquat Ethoquad DAET T/25 23-01 345 7. 5 7. 5 23-02 349 7. 5 7. 5 Glyphosate concentration in each of compositions 23-01 and 23-02 was approximately 30% a. e. by weight.

Velvetleaf (Abutilon theophrasti, ABUTH) plants were grown and treated by the standard procedures given above, except that 6 replicate pots were subjected to each treatment, applied in two sets of three pots. One set of three pots for each treatment was subjected to simulated rain, using an overhead irrigator, in the amount of 6 mm, one hour after application of plant treatment compositions. Applications of plant treatment compositions were made 18 days after planting ABUTH, and evaluation of herbicidal effectiveness was done 14 days after application.

In addition to compositions 23-01 and 23-02, composition 12-01 of Example 12 was included in the test of this Example. MON 0139 and Roundup Ultra were diluted and applied

as comparative treatments. Also included as a comparative treatment was MON 0139, diluted and applied in tank-mixture with the organosilicone surfactant Silwet L-77 of OSi Specialties Group of Witco Corp., at 0.5% by volume. Results, averaged for all replicates of each treatment, are shown in Table 23b.

Table 23b Composition Glyphosate rate % Inhibition, ABUTH g a. e./ha no rain rain MON 0139 250 19 0 500 63 0 750 78 9 2507843Roundup#Ultra 500 98 50 750 98 69 MON 0139 + Silwet L-77 250 75 69 500 94 95 750 98 97 12-01 250 82 53 500 98 80 750 99 80 23-01 250 60 10 500 92 46 750 98 62 23-02 6884 500 98 94 750 98 98 Composition 23-02 of the present invention exhibited a remarkably high degree of rainfastness in this test, equal to that of the rainfastness standard MON 0139 + Silwet L-77.

Composition 12-01 of the present invention also exhibited rainfastness markedly superior to that

of the commercial standard Roundup (S) Ultra. Comparative composition 23-01 showed very poor rainfastness.

The test of this Example was repeated. Applications of plant treatment compositions were again made 18 days after planting ABUTH, and evaluation of herbicidal effectiveness was done 14 days after application. Results, averaged for all replicates of each treatment, are shown in Table 23c.

Table 23c Composition Glyphosate rate % Inhibition, ABUTH g a. e./ha no rain rain MON 0139 250 2 0 500 73 0 750 81 2 Roundup (t Ultra 250 87 53 500 98 65 750 98 67 MON 0139 + Silwet L-77 250 88 93 500 99 98 750 99 99 12-01 250 78 55 500 97 81 750 99 91 23-01 250 84 12 500 98 63 750 87 63 23-02 250 87 67 500 98 94 750 99 96 Results of this repeat test were consistent with those of the first test shown in Table 23b.

Example 24 Samples of seven liquid aqueous concentrate compositions 24-01 to 24-07 were examined by proton NMR spectroscopy using the pulse field gradient method of Wu et al., op. cit. to measure diffusion rates. The excipient ingredients of the compositions were as detailed in Table 24. Each composition contained glyphosate isopropylammonium salt at a concentration of 30% a. e. by weight. Compositions were prepared by procedures hereinabove described. Three separate preparations of composition 24-04 and two separate preparations of composition 24-05 were studied. The alkylether surfactant was Plurafac A-38 of BASF in all compositions except 24-01, in which the very similar HetoxolTM CS of Heterene was used. The amine surfactant in all compositions was MON 0818 of Monsanto. The coupling agent was DMSO in all compositions except 24-01, wherein the DMSO was replaced by urea.

Table 24 Concentrate Weight % composition alkylether amine soybean butyl coupling surfactant surfactant lecithin stearate agent 8.55.02.51.024-014.5 24-02 6. 0 6. 0 6. 0 2. 5 0. 5 24-03 4. 5 6. 0 6. 0 2. 5 0. 5 24-04 4. 5 4. 5 5. 0 2. 5 0. 5 24-05 4. 5 4. 5 6. 0 3. 0 0. 5 24-06 4. 5 4. 5 6. 0 2. 5 0. 5 24-07 6. 0 4. 5 6. 0 2. 5 0. 5

A 200-500 1 sample of each composition was placed in an NMR tube for diffusion measurement using a Nalorac diffusion probe having a diffusion coil capable of producing a linear field gradient across the sample of about 250 gauss/cm in response to a 20 amp current pulse. The current pulse was generated by a Perfora gradient driver inside the console of a Varian Unity 400 spectrometer. Proton NMR spectra were recorded as a function of increasing field gradient, using bipolar pulses and LEDS pulse sequence.

Amplitude of the glyphosate resonance was measured in each spectrum, and the natural logarithm of amplitude was plotted against the square of field gradient. An illustrative example of such a plot is shown in Figure 1, for composition 24-04. Two straight-line components, corresponding to a"free"pool and an"entrapped"pool of glyphosate, were clearly distinguishable in all compositions except 24-01, which had no measurable"entrapped"pool. In compositions having an"entrapped"pool, the diffusion coefficients of this pool were extremely low, of the order of 10''° cm2/s, indicating strong binding or entrapment of the glyphosate by supramolecular structures in these compositions. Except in composition 24-01, the"entrapped" pool comprised about 20% to about 80% of all glyphosate present.

The fraction of the glyphosate in the"entrapped"pool was found to be correlated with the weight ratio of lecithin to MON 0818. As illustrated in Figure 2, the higher the lecithin to MON 0818 ratio, the greater was the fraction of glyphosate in the"entrapped"pool.

Example 25 Solid water-soluble granule compositions were prepared containing glyphosate ammonium salt, glufosinate ammonium salt and excipient ingredients as shown in Table 25a.

Compositions 25-01 to 25-06 were prepared by the procedure described in Example 16, with the addition to the wet mix of glufosinate ammonium salt in the desired amount.

Table 25a Granule Weight % composition Glyphosate Glufosinate Plurafac amine a. e. a. e. A-38 surfactant A 25-01 66. 1 2. 0 21. 0 25-02 66. 1 2. 0 10. 5 10. 5 25-03 66. 1 2. 0 5. 3 15. 7 25-04 65. 0 3. 0 21. 0 25-05 65. 0 3. 0 10. 5 10. 5 25-06 65. 0 3. 0 5. 3 15. 7 Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of

plant treatment compositions were made 17 days after planting ABUTH and ECHCF.

Evaluation of early symptom development was done 3 days after application (DAA), and evaluation of herbicidal effectiveness was done 17 days after application.

Roundup Ultra was diluted and applied as a comparative treatment. Results, averaged for all replicates of each treatment, are shown in Table 25b.

Table 25b Concentrate composition Glyphosate and % Inhibition glufosinate rates ABUTH ECHCF g a. e./ha 3 DAA 17 DAA 3 DAA 17 DAA 100+0034037Roundup#Ultra 200 + 0 0 81 0 76 400+0 0 97 0 93 600 + 0 0 99 0 94 +303003625-01100 200 + 6 0 76 0 75 400+12 2 96 0 86 600 + 18 14 98 6 93 +307104425-02100 200 + 6 0 92 0 77 121095188400+ 181696691600+ +306604925-03100 200 + 6 0 89 0 81 400+12 12 97 8 98 600 + 18 17 99 14 93 +4.503203525-04100 200 + 9 0 80 0 77 181292483400+ 600 + 27 17 91 9 85 Concentrate composition Glyphosate and % Inhibition glufosinaterates ABUTH ECHCF g a. e./ha 3 DAA 17 DAA 3 DAA 17 DAA 25-OS 100 + 4. 5 0 67 0 47 200 + 9 5 86 0 75 400+ 18 13 91 88 600 + 27 20 94 l 7 92 25-06 100+4. 5 0 70 0 53 200 + 9 8 90 0 83 400 + 18 14 91 11 85 600 + 27 18 91 15 88

Compositions 25-02 and 25-03 of the invention gave superior herbicidal effectiveness to composition 25-01 which contained amine surfactant A but did not contain the alkylether surfactant Plurafac A-38. Likewise, compositions 25-05 and 25-06 of the invention gave superior herbicidal effectiveness to composition 25-04.

Example 26 Aqueous suspension concentrate compositions were prepared containing glyphosate isopropylammonium salt, oxyfluorfen and surfactants as shown in Table 26a. Surfactant J is the tallowamine-based surfactant used in formulating Roundup# Ultra.

Compositions 26-01 and 26-02 were prepared by the following procedure. A 10% by weight aqueous solution of Surfactant J was first prepared. To this solution in a wide-mouthed jar was added oxyfluorfen powder, technical grade (95%) in an amount calculated to provide a 41% by weight suspension of oxyfluorfen active ingredient (a. i.). The jar was then placed in an Eiger mill where the suspension was milled for 2 hours at 3000 rpm in a cooling bath at 10°C.

Particle size analysis of the resulting milled oxyfluorfen showed a volume mean diameter of 2.5 m and a volume median diameter of 1.7 m. The surfactant, in this case Surfactant J, was present to facilitate the milling operation.

Glyphosate isopropylammonium salt in the form of MON 0139 (46% by weight glyphosate a. e.) was mixed with the 41% milled oxyfluorfen prepared as above in a weight ratio

of glyphosate a. e. to oxyfluorfen a. i. of 12: 1. (As the milled oxyfluorfen contained approximately 6% by weight Surfactant J, a small amount of this surfactant was contributed along with oxyfluorfen to the final composition ; the amount is believed small enough to be negligible in affecting herbicidal effectiveness.) Also added were surfactants selected as shown in Table 26a, colloidal particulate silica (a blend of Aerosil 380 and AerosilTM MOX-80 of Degussa), propylene glycol, sodium sulfite and water in the amounts listed below (percentages are by weight): MON 0139 (46% glyphosate a. e.) 67.00% oxyfluorfen (41% milled) 6.30% AerosilTM 380 1.45% AerosilTM MOX-80 0.25% surfactant (s) and coupling agent (see Table 26a) sodium sulfite 0.20% water to 100.00% The above ingredients were thoroughly agitated for about 5 minutes or until a homogeneous suspension was formed. Ethoquad T/20 of Akzo is a polyoxyethylene quaternary tallowalkylmethylammonium chloride surfactant having an average of about 10 oxyethylene units per molecule. Plurafac A-38 of BASF is a polyoxyethylene C16-18 alkylether having an average of about 27 oxyethylene units per molecule. Composition 26-01 was included for comparative purposes only.

Table 26a Concentrate Weight % composition Glyphosate Oxyfluorfen Plurafac Ethoquad Surfactant Coupling a.e. a.i. A-38 T/20 agent 2.614.50.2226-0130.8 26-02 30.8 2.6 3.0 7.0 1.0@ 'excluding minor amount added to oxyfluorfen during milling 2 monoethanolamine 3 propylene glycol

Filaree (Erodium sp., EROSS) and annual bluegrass (Poa annua, POAAN) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 36 days after planting EROSS and POAAN. Evaluation of early symptom development was done 4 days after application (DAA), and evaluation of herbicidal effectiveness was done 20 days after application.

Roundup Ultra was diluted and applied as a comparative treatment, both alone and in tank-mixture with Goal@ 2XL herbicide of Rohm & Haas, an emulsifiable concentrate formulation of oxyfluorfen. The tank-mixture was prepared to give a 12: 1 weight ratio of glyphosate a. e. to oxyfluorfen a. i. Results, averaged for all replicates of each treatment, are shown in Table 26b.

Table 26b Concentrate composition Glyphosate and % Inhibition oxyfluorfenrates EROSS POAAN g a. e., a. i./ha 4 DAA 20 DAA 4 DAA 20 DAA Roundup (R) Ultra400+055050 0072063600+ 800 + 0 3 93 0 78 1000 + 0 8 93 2 88 +400+3310331263Roundup#Ultra Goal@ 2XL tank-mixture 600 + 50 17 45 13 65 800 + 67 20 65 17 68 1000+83 20 72 18 75 +3382254826-01400 5018531073600+ 800 + 67 22 82 10 75 1000 + 83 30 92 10 75 26-02 331050858+ 600+50 15 60 8 68 800 + 67 20 90 8 77 8325938831000+

Significant antagonism to the herbicidal effectiveness of glyphosate, especially on EROSS, was seen in this test with addition of oxyfluorfen. Composition 26-02 of the invention gave greatly reduced antagonism by comparison with the tank-mixture and also showed less antagonism than composition 26-01.

Example 27 Solid water-dispersible granule compositions were prepared containing glyphosate ammonium salt, oxyfluorfen and surfactants as shown in Table 26a.

Compositions 27-01 and 27-02 were prepared by the following procedure. In a food mixer were blended dry ammonium glyphosate powder (MON 8750 of Monsanto), a small amount of water (about 5 g per 100 g of all other ingredients), surfactants and ammonium sulfate as shown in Table 27a, and 41% milled oxyfluorfen prepared as described in Example 26. The weight ratio of glyphosate a. e. to oxyfluorfen a. i. was 12: 1. After thorough blending, the resulting wet mix was extruded and dried as described in Example 16.

Compositions 27-03 and 27-04 were prepared by a similar procedure except that oxyfluorfen (technical grade, 95%) was melted and added to the wet mix instead of 41 % milled oxyfluorfen. The amount of water added was increased to 7-8 g per 100 g of all other ingredients.

Table 27a Granule Weight % composition Glyphosate Oxyfluorfen Plurafac Ethoquad Ammonium a.e. a.i. sulfateT/20 3.16.256.2540.527-0137.0 27-02 37.0 3.1 3.12 9.38 40.5 27-03 37. 0 3. 1 6. 25 6. 25 40. 5 27-04 37. 0 3. 1 3. 12 9. 38 40. 5 Velvetleaf (Abutilon theophrasti, ABUTH) and Japanese millet (Echinochloa crus-galli, ECHCF) plants were grown and treated by the standard procedures given above. Applications of plant treatment compositions were made 18 days after planting ABUTH and ECHCF.

Evaluation of early symptom development was done 2 days after application (DAA), and evaluation of herbicidal effectiveness was done 18 days after application.

Roundup Ultra was diluted and applied as a comparative treatment, both alone and in tank-mixture with Goal (g) 2XL herbicide of Rohm & Haas, an emulsifiable concentrate formulation of oxyfluorfen. The tank-mixture was prepared to give a 12: 1 weight ratio of glyphosate a. e. to oxyfluorfen a. i. Also included in this test were compositions 26-01 (as a comparative treatment) and 26-02 of Example 26. Results, averaged for all replicates of each treatment, are shown in Table 27b.

Table 27b Concentrate composition Glyphosate and % Inhibition oxyfluorfenrates ABUTH ECHCF g a. e., a. i./ha 2 DAA 18 DAA 2 DAA 18 DAA 100+0053037Roundup#Ultra 200+0 0 83 0 72 400 + 0 0 96 0 78 600 + 0 0 100 0 87 Roundup@ Ultra + 100 + 8 12 17 8 10 Goal (g) 2XL tank-mixture 200 + 17 18 57 12 70 400 + 33 27 72 18 77 5032772582600+ +8104773026-01100 200 + 17 15 70 10 73 400 + 33 18 85 17 78 600+50 20 87 18 87 26-02 100+8 8 70 7 70 200 + 17 13 90 10 75 400 + 33 15 98 13 82 600+ 50 17 99 20 90 Concentrate composition Glyphosate and % Inhibition oxyfluorfenrates ABUTH ECHCF g a. e., a. i./ha 2 DAA 18 DAA 2 DAA 18 DAA +875356827-01100 171278775200+ 400 + 33 13 88 12 82 600 + 50 18 90 17 94 27-02 100+8 5 67 5 72 200+178831275 400 + 33 12 95 15 85 600 + 50 15 96 15 90 +8104536527-03100 1718781075200+ 400 + 33 22 85 15 80 5022931883600+ +878037227-04100 200+ 17 10 90 10 75 400 + 33 13 98 12 82 600+50 15 99 17 89

Significant antagonism to the herbicidal effectiveness of glyphosate, especially on ABUTH, was seen in this test with addition of oxyfluorfen. Compositions 26-02 and 27-01 to 27-04 of the invention gave greatly reduced antagonism by comparison with the tank-mixture and also showed less antagonism than composition 26-01.

The preceding description of specific embodiments of the present invention is not intended to be a complete list of every possible embodiment of the invention. Persons skilled in this field will recognize that modifications can be made to the specific embodiments described here that remain within the scope of the present invention.