METHOD FOR ENHANCING FORMATION OF SPHEROID AND CULTURE MEDIUM FOR ENHANCING FORMATION OF SPHEROID

WIPO Patent Application WO/2011/077894

A1

Disclosed is a cell cultivation method which is capable of forming a spheroid by simple processes in a short term. Also disclosed is a culture medium for enhancing the formation of a spheroid, which can be used for the cell cultivation method. By culturing cells on a culture substrate, which has a recessed and projected structure that functions as a cell adhesion surface, using a cell culture medium that contains a predetermined extracellular matrix component, the proliferation and growth of monolayer cells as well as separation and collapse of a spheroid are prevented, thereby extremely effectively enhancing the formation of the spheroid.

WO/2004/018646	CONSERVED AND SPECIFIC STREPTOCOCCAL GENOMES
WO/2002/044422	METHOD FOR ULTRA-HIGH RESOLUTION MAPPING OF GENES AND DETERMINATION OF GENETIC NETWORKS AMONG GENES UNDERLYING PHENOTYPIC TRAITS
WO/1987/005332	RECOMBINANT HUMAN ENDOTHELIAL CELL GROWTH FACTOR

KOBAYASHI MAKI (JP)
YOSHIDA KAORI (JP)
WAKI ATSUO (JP)
TANAKA SATORU (JP)
YOSHII YUKIE (JP)

PCT/JP2010/071002

June 30, 2011

November 25, 2010

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SCIVAX CORP (JP)
KOBAYASHI MAKI (JP)
YOSHIDA KAORI (JP)
WAKI ATSUO (JP)
TANAKA SATORU (JP)
YOSHII YUKIE (JP)

C12N5/00; C12N5/02

JP2008022743A	2008-02-07
JP4159103B2	2008-10-01
JP2009515558A	2009-04-16
JP2008289362A	2008-12-04

MASAMI HIROYAMA ET AL.: "Nano Technology o Mochiita Kakushinteki 3-jigen Saibo Baiyoho (Nanoculture-ho) ni yoru Saisei Iryo eno Koken", EXPERIMENTAL MEDICINE, vol. 26, no. 18, 2008, pages 2942 - 2943

OKUDA Noritsugu (JP)
Okuda 律次 (JP)

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