Title:
METHOD OF ENHANCING STRESS TOLERANCE OF MONOCOTYLEDONOUS PLANTS
Document Type and Number:
WIPO Patent Application WO/2017/002070
Kind Code:
A3
Abstract:
A method is provided for producing a transformed plant of the family Poaceae. One or more genes selected from the group consisting of OTS1, OTS2 and ICE1 are introduced into the genome of the plant, resulting in transformed plants having enhanced tolerance to an abiotic stress compared to untransformed plants. The gene can be under the control of a drought inducible promoter, such as the Rab17 promoter. Examples of the abiotic stress are drought, heat, cold and salinity. The plant can be a cereal or grass, such as crops or forage grasses including wheat, barley, sorghum, maize, sugarcane, oats, rye, triticale and commercial fodder grass species. The invention also extends to a vector for transforming the plants and to transformed plants and plant parts.
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Inventors:
BOTHA-OBERHOLSTER ANNA-MARIA (ZA)
VAN DER VYVER CHRISTELL (ZA)
LE ROUX MARLON LUKE (ZA)
VAN DER VYVER CHRISTELL (ZA)
LE ROUX MARLON LUKE (ZA)
Application Number:
PCT/IB2016/053936
Publication Date:
April 13, 2017
Filing Date:
June 30, 2016
Export Citation:
Assignee:
UNIV STELLENBOSCH (ZA)
International Classes:
C12N15/82; A01H5/04; A01H5/10
Foreign References:
| US8173866B1 | 2012-05-08 |
Other References:
CONTI L ET AL: "Small Ubiquitin-Like Modifier Proteases OVERLY TOLERANT TO SALT1 and-2 Regulate Salt Stress Responses in Arabidopsis", THE PLANT CELL, AMERICAN SOCIETY OF PLANT BIOLOGISTS, US, vol. 20, no. 10, 20 October 2008 (2008-10-20), pages 2894 - 2908, XP002713560, ISSN: 1040-4651, [retrieved on 20081010], DOI: 10.1105/TPC.108.058669
CONTI LUCIO ET AL: "OTS1 and OTS2 SUMO proteases link plant development and survival under salt stress.", PLANT SIGNALING & BEHAVIOR MAR 2009, vol. 4, no. 3, March 2009 (2009-03-01), pages 225 - 227, XP002761518, ISSN: 1559-2324
FARQUHARSON KATHLEEN L: "OTS1/2-Dependent DeSUMOylation Boosts Salt Tolerance", PLANT CELL, vol. 20, no. 10, October 2008 (2008-10-01), pages 2542, XP002761519, ISSN: 1040-4651
KATIYAR-AGARWAL SUREKHA ET AL: "Heat-tolerant basmati rice engineered by over-expression of hsp101", PLANT MOLECULAR BIOLOGY, SPRINGER, DORDRECHT, NL, vol. 51, no. 5, 1 March 2003 (2003-03-01), pages 677 - 686, XP002292846, ISSN: 0167-4412, DOI: 10.1023/A:1022561926676
SEONG ET AL: "Regulations of marker genes involved in biotic and abiotic stress by overexpression of the AtNDPK2 gene in rice", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 363, no. 1, 22 September 2007 (2007-09-22), pages 126 - 132, XP022265399, ISSN: 0006-291X, DOI: 10.1016/J.BBRC.2007.08.147
ZHANG YI-YUE ET AL: "Arabidopsis SDIR1 enhances drought tolerance in crop plants.", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY AUG 2008, vol. 72, no. 8, August 2008 (2008-08-01), pages 2251 - 2254, XP002761520, ISSN: 1347-6947
XIANG DIAN-JUN ET AL: "Transformation of ICE1 gene mediated by Agrobacterium improves cold tolerance in transgenic rice", ZHONGGUO SHUIDAO KEXUE, ZHONGGUO SHUIDAO YANJIUSUO, CN, vol. 21, no. 5, 1 September 2007 (2007-09-01), pages 482 - 486, XP008181551, ISSN: 1001-7216
CONTI LUCIO ET AL: "OTS1 and OTS2 SUMO proteases link plant development and survival under salt stress.", PLANT SIGNALING & BEHAVIOR MAR 2009, vol. 4, no. 3, March 2009 (2009-03-01), pages 225 - 227, XP002761518, ISSN: 1559-2324
FARQUHARSON KATHLEEN L: "OTS1/2-Dependent DeSUMOylation Boosts Salt Tolerance", PLANT CELL, vol. 20, no. 10, October 2008 (2008-10-01), pages 2542, XP002761519, ISSN: 1040-4651
KATIYAR-AGARWAL SUREKHA ET AL: "Heat-tolerant basmati rice engineered by over-expression of hsp101", PLANT MOLECULAR BIOLOGY, SPRINGER, DORDRECHT, NL, vol. 51, no. 5, 1 March 2003 (2003-03-01), pages 677 - 686, XP002292846, ISSN: 0167-4412, DOI: 10.1023/A:1022561926676
SEONG ET AL: "Regulations of marker genes involved in biotic and abiotic stress by overexpression of the AtNDPK2 gene in rice", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 363, no. 1, 22 September 2007 (2007-09-22), pages 126 - 132, XP022265399, ISSN: 0006-291X, DOI: 10.1016/J.BBRC.2007.08.147
ZHANG YI-YUE ET AL: "Arabidopsis SDIR1 enhances drought tolerance in crop plants.", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY AUG 2008, vol. 72, no. 8, August 2008 (2008-08-01), pages 2251 - 2254, XP002761520, ISSN: 1347-6947
XIANG DIAN-JUN ET AL: "Transformation of ICE1 gene mediated by Agrobacterium improves cold tolerance in transgenic rice", ZHONGGUO SHUIDAO KEXUE, ZHONGGUO SHUIDAO YANJIUSUO, CN, vol. 21, no. 5, 1 September 2007 (2007-09-01), pages 482 - 486, XP008181551, ISSN: 1001-7216
Attorney, Agent or Firm:
VON SEIDELS INTELLECTUAL PROPERTY ATTORNEYS (ZA)
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