Title:
METHOD FOR ENZYMATIC MODIFICATION OF N-TERMINUS OF PROTEIN
Document Type and Number:
WIPO Patent Application WO/2007/061128
Kind Code:
A1
Abstract:
The object is to provide a protein having the modified N-terminus in a large quantity. Disclosed is a method for preparation of a protein having an N-terminus that has a modified phenylalanine residue. According to the method, in a protein having an Arg or Lys residue at the N-terminus, a modified phenylalanine residue is added to the N-terminal arginine residue with a modified phenylalanine tRNAPhe using a leucine/phenylalanine-tRNA protein transferase (LFPT) capable of adding a Leu or Phe residue to the terminus, whereby a protein having a modified phenylalanine residue at the N-terminus can be prepared.
Inventors:
NISHIKAWA KAZUYA (JP)
YOKOGAWA TAKASHI (JP)
OHNO SATOSHI (JP)
YOKOGAWA TAKASHI (JP)
OHNO SATOSHI (JP)
Application Number:
PCT/JP2006/323879
Publication Date:
May 31, 2007
Filing Date:
November 22, 2006
Export Citation:
Assignee:
UNIV GIFU (JP)
NISHIKAWA KAZUYA (JP)
YOKOGAWA TAKASHI (JP)
OHNO SATOSHI (JP)
NISHIKAWA KAZUYA (JP)
YOKOGAWA TAKASHI (JP)
OHNO SATOSHI (JP)
International Classes:
C07K1/13; C12N9/00; C12N15/09; C12P21/00; C12P13/04; C12P21/06
Other References:
KUNO A. ET AL.: "Leucyl/phenylalanyl (L/F)-tRNA-protein transferase-mediated N-terminal specific labelling of a protein in vitro", NUCLEIC ACIDS RES., no. SUPPL., 2003, pages 259 - 260, XP003013352
SHARMA N. ET AL.: "Efficient introduction of aryl bromide functionality into proteins in vivo", FEBS LETT., vol. 467, no. 1, 2000, pages 37 - 40, XP004260918
CHIN J.W. ET AL.: "An expanded eukaryotic genetic code", SCIENCE, vol. 301, no. 5635, 2003, pages 964 - 967, XP002996001
SAKAMOTO K. ET AL.: "Ikita Saibo de Chotanpakushitsu o Tsukuru - Hitennen Amino Acid o Tanpakushitsu ni Donyu suru Hoho", CHEMISTRY, vol. 60, no. 4, 25 March 2005 (2005-03-25), pages 70 - 71, XP003013353
ABRAMOCHKIN G. ET AL.: "The leucyl/phenylalanyl-tRNA-protein transferase. Overexpression and characterization of substrate recognition, domain structure, and secondary structure", J. BIOL. CHEM., vol. 270, no. 35, 1995, pages 20621 - 20628, XP003013354
SANNI A. ET AL.: "Structure and expression of the genes encoding the alpha and beta subunits of yeast phenylalanyl-tRNA synthetase", J. BIOL. CHEM., vol. 263, no. 30, 1988, pages 15407 - 15415, XP003013355
SHARMA N. ET AL.: "Efficient introduction of aryl bromide functionality into proteins in vivo", FEBS LETT., vol. 467, no. 1, 2000, pages 37 - 40, XP004260918
CHIN J.W. ET AL.: "An expanded eukaryotic genetic code", SCIENCE, vol. 301, no. 5635, 2003, pages 964 - 967, XP002996001
SAKAMOTO K. ET AL.: "Ikita Saibo de Chotanpakushitsu o Tsukuru - Hitennen Amino Acid o Tanpakushitsu ni Donyu suru Hoho", CHEMISTRY, vol. 60, no. 4, 25 March 2005 (2005-03-25), pages 70 - 71, XP003013353
ABRAMOCHKIN G. ET AL.: "The leucyl/phenylalanyl-tRNA-protein transferase. Overexpression and characterization of substrate recognition, domain structure, and secondary structure", J. BIOL. CHEM., vol. 270, no. 35, 1995, pages 20621 - 20628, XP003013354
SANNI A. ET AL.: "Structure and expression of the genes encoding the alpha and beta subunits of yeast phenylalanyl-tRNA synthetase", J. BIOL. CHEM., vol. 263, no. 30, 1988, pages 15407 - 15415, XP003013355
Attorney, Agent or Firm:
HIRAKI, Yusuke et al. (3-20 Toranomon 4-chome, Minato-k, Tokyo 01, JP)
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