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Title:
METHOD FOR ENZYMATIC PREPARATION OF R-3-AMINOBUTYRIC ACID
Document Type and Number:
WIPO Patent Application WO/2019/062222
Kind Code:
A1
Abstract:
An R-3-aminobutyric acid preparation method with high efficiency and high stereoselectivity. The method comprises using aspartase with stereoisomeric catalytic activity derived from Escherichia coli to efficiently convert butenoic acid into R-3-aminobutyric acid. After only 24 h of reaction, the conversion rate is as high as ≥ 98%, and the ee value is ≥ 99.9%. The conversion efficiency is greatly improved, the reaction time is shortened, and the production costs are reduced. The method features a high yield, a high conversion rate, low costs, a short production cycle, a simple process, ease of enlargement, suitability for mass production and the like.

Inventors:
SUN CHUANMIN (CN)
CHENG ZHANBING (CN)
JIAO JIANGHUA (CN)
DING SHAONAN (CN)
TIAN ZHENHUA (CN)
Application Number:
PCT/CN2018/092010
Publication Date:
April 04, 2019
Filing Date:
June 20, 2018
Export Citation:
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Assignee:
ABIOCHEM BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12P13/04; C07K14/245; C12N1/19; C12N9/88
Foreign References:
JP2003189863A2003-07-08
US7256021B22007-08-14
CN104370755A2015-02-25
Other References:
VOGEL, A. ET AL.: "Converting Aspartase into a beta-Amino Acid Lyase by Cluster Screening", CHEMCATCHEM., vol. 6, 31 December 2014 (2014-12-31), pages 965 - 968, XP055588883
WANG, LIJUAN ET AL.: "Enhancement of the Activity of L-Aspartase from Escherichia Coli. W by Directed Evolution", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 276, 31 December 2000 (2000-12-31), pages 346 - 349, XP055588888
ZHANG, HONGYING ET AL.: "Enhancement of the Stability and Activity of Aspartase by Random and Site-Directed Mutagenesis", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 192, no. 1, 15 April 1993 (1993-04-15), pages 15 - 21, XP024768071, DOI: doi:10.1006/bbrc.1993.1375
DATABASE GenBank 8 June 2009 (2009-06-08), LEOPOLD, S.R. ET AL.: "Aspartate Ammonia-Lyase[Escherichia Coli", XP055588898, Database accession no. ACI73571.1
TETRAHEDRON: ASYMMETRY, vol. 18, 2007, pages 1554 - 1566
CHEMCATCHEM, vol. 8, 2016, pages 1226 - 1232
CHEMCATCHEM, vol. 6, 2014, pages 965 - 968
WATSON ET AL.: "Sequence Analysis in Molecular Biology", 1987, THE BENJAMIN/CUMMINGS PUB. CO., pages: 224
CARILLO, H.LIPMAN, D., SIAM J. APPLIED MATH ., vol. 48, 1988, pages 1073
"Biocomputing; Biocomputing: Informatics and Genome Projects", 1993, ACADEMIC PRESS
"Computer Analysis of Sequence Data", 1994, HUMANA PRESS
"Sequence Analysis Primer", 1991, M STOCKTON PRESS
ALTSCHUL, S. ET AL.: "Molecule Clone: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP 3680340A4
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
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