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Title:
METHOD FOR ENZYMATIC RESOLUTION OF CHIRAL SUBSTANCE
Document Type and Number:
WIPO Patent Application WO/2020/119280
Kind Code:
A1
Abstract:
The present invention relates to the technical field of bioengineering and food, and relates to a method for enzymatic resolution of a chiral substance, comprising the following steps: (1) preparing an enzyme solution having a lipase concentration of 1-3000 U/mL, and adding a soluble salt, a hydrophilic solvent, and a hydrophobic solvent into the enzyme solution to prepare a three-liquid phase system, the hydrophobic solvent containing an ester or amide compound composed of a racemic chiral substance; and (2) enabling enzyme-catalyzed reaction of the three-liquid phase system under a stirring condition, and after the reaction ends, standing or centrifuging until the three-liquid phase system is divided into three layers, i.e., an upper liquid layer, a middle liquid layer, and a lower liquid layer from top to bottom in sequence, the hydrolyzed single optically chiral product being mainly enriched in the middle liquid layer or the lower liquid layer, and an upper liquid layer product being another ester or amide product containing the single optically chiral product. The method has the advantages of being small in energy consumption, high in raw material utilization, mild in reaction condition, etc., and the problem that existing enzymatic chiral resolution is low in efficiency, poor in chiral selectivity, low in recovery rate, and difficult in industrialization is solved.

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Inventors:
LI ZHIGANG (CN)
SU JINFEN (CN)
YANG BO (CN)
WANG YONGHUA (CN)
LI ZHENCHENG (CN)
CHEN HUAYONG (CN)
Application Number:
PCT/CN2019/113139
Publication Date:
June 18, 2020
Filing Date:
October 25, 2019
Export Citation:
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Assignee:
UNIV SOUTH CHINA TECH (CN)
International Classes:
C12P13/02; C12P7/62; C12P41/00
Foreign References:
CN109628508A2019-04-16
US5928933A1999-07-27
CN106520898A2017-03-22
CN1329671A2002-01-02
US5342780A1994-08-30
CN105506052A2016-04-20
Other References:
CHEN, HUA ET AL.: "Hydrolysis of Olive Oil by Lipase in Three Liquid Phase System", CHINA OILS AND FATS, vol. 43, no. 7, 18 July 2018 (2018-07-18), pages 51 - 56
Attorney, Agent or Firm:
GUANGZHOU HUAXUE INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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