Title:
METHOD FOR ENZYMATIC SYNTHESIS OF CHLORAMPHENICOL INTERMEDIATE
Document Type and Number:
WIPO Patent Application WO/2019/140687
Kind Code:
A1
Abstract:
Disclosed in the present invention is a method for enzymatic synthesis of chloramphenicol intermediate. In the method, nitrobenzaldehyde and glycine are used as substrates, and are reacted under the catalysis of aldolase to obtain a chloramphenicol intermediate. According to the method for preparing a chloramphenicol intermediate provided by the present invention, from inexpensive raw materials that are easily obtained, a chloramphenicol intermediate is obtained by reacting substrates nitrobenzaldehyde and glycine using an aldolase. By means of the reaction, the costs of raw materials are reduced, and the disadvantages in the prior art, such as complicated operation, large pollution, and low yield are overcome. The method provided by the present invention has high conversion rate and high yield of target products, and simplifies the process for preparing chloramphenicol intermediates; the aldolase used can be easily obtained by fermentation using Escherichia coli; the method is lower in production costs and better in product quality than a chemical method, and is suitable for industrial production.
Inventors:
FU RONGZHAO (CN)
LIU LIHUI (CN)
ZHANG GUIWEI (CN)
JIANG MING (CN)
LIU LIHUI (CN)
ZHANG GUIWEI (CN)
JIANG MING (CN)
Application Number:
PCT/CN2018/073631
Publication Date:
July 25, 2019
Filing Date:
January 22, 2018
Export Citation:
Assignee:
BONTAC BIO ENG SHENZHEN CO LTD (CN)
BONTAC INST OF GREEN BIOCATALYSIS (CN)
BONTAC INST OF GREEN BIOCATALYSIS (CN)
International Classes:
C12P13/04; C12P7/22; C12P7/42
Other References:
J. STEINREIBER: "Threonine Aldolases-an Emerging Tool for Organic Synthe- sis", TETRAHEDRON, vol. 63, 8 December 2006 (2006-12-08), pages 4, ISSN: 0040-4020
TEIJI KIMURA: "Enzymatic Synthesis of beta-Hydroxy-alpha-amino Acids Based on Recombinant D- and L-Threonine Aldolases", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 119, 31 December 1997 (1997-12-31), pages 49, XP055556922, ISSN: 0002-7863
DATABASE Protein 12 January 2018 (2018-01-12), "Multispecies: Low Specificity L-Threonine Aldolase 3[Pseudomonas", XP055624878, retrieved from NCBI Database accession no. WP_038998324.1
DATABASE Protein 13 May 2017 (2017-05-13), "Low Specificity L-Threonine Aldolase [Pseudomonas Aeruginosa", XP055624881, retrieved from NCBI Database accession no. WP_023116837.1
DATABASE Protein 14 May 2017 (2017-05-14), "Multispecies: Low Specificity L-Threonine Aldolase [Pseudomonas", XP055624885, retrieved from NCBI Database accession no. WP_010951681.1
DATABASE Protein 27 March 2017 (2017-03-27), "TPA: Threonine Aldolase GLY1 [Saccharomyces Cerevisiae S288C", XP055624891, retrieved from NCBI Database accession no. DAA07608.1
TEIJI KIMURA: "Enzymatic Synthesis of beta-Hydroxy-alpha-amino Acids Based on Recombinant D- and L-Threonine Aldolases", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 119, 31 December 1997 (1997-12-31), pages 49, XP055556922, ISSN: 0002-7863
DATABASE Protein 12 January 2018 (2018-01-12), "Multispecies: Low Specificity L-Threonine Aldolase 3[Pseudomonas", XP055624878, retrieved from NCBI Database accession no. WP_038998324.1
DATABASE Protein 13 May 2017 (2017-05-13), "Low Specificity L-Threonine Aldolase [Pseudomonas Aeruginosa", XP055624881, retrieved from NCBI Database accession no. WP_023116837.1
DATABASE Protein 14 May 2017 (2017-05-14), "Multispecies: Low Specificity L-Threonine Aldolase [Pseudomonas", XP055624885, retrieved from NCBI Database accession no. WP_010951681.1
DATABASE Protein 27 March 2017 (2017-03-27), "TPA: Threonine Aldolase GLY1 [Saccharomyces Cerevisiae S288C", XP055624891, retrieved from NCBI Database accession no. DAA07608.1
Attorney, Agent or Firm:
JIAQUAN IP LAW FIRM (CN)
Download PDF: