METHOD FOR ESTIMATING MICROBIAL DENSITY AND MICROARRAY FOR ESTIMATING MICROBIAL DENSITY

WIPO Patent Application WO/2019/044126

A1

[Problem] To enable the estimation of the density of microorganisms in an environment. [Solution] A method for estimating the density of microorganisms, said method comprising using the after-mentioned probes a and b, measuring signal values which indicate the hybridization levels of the probes with a polynucleotide derived from the target microorganisms, and determining to which of the after-mentioned patterns 1 to 3 the microbial density belongs on the basis of the signal values and a threshold. Probe a: A probe which comprises a base sequence specifically hybridizable with the polynucleotide and shows a signal value being equal to or higher than the threshold at density α. Probe(s) b: One or more probes which comprise a base sequence specifically hybridizable with the polynucleotide and show a signal value being equal to or higher than the threshold at density β that is lower than density α. Pattern 1: When both of the signal values of probes a and b are equal to or higher than the threshold, the density is equal to or higher than α. Pattern 2: When the signal value of probe a is lower than the threshold and the signal value of probe b is equal to or higher than the threshold, the density is equal to or higher than β and lower than α. Pattern 3: When both of the signal values of probes a and b are lower than the threshold, the density is lower than β.

OTSU TAKAYOSHI (JP)
DOUSHI HIROKI (JP)
ISSHIKI ATSUNORI (JP)

PCT/JP2018/023392

March 07, 2019

June 20, 2018

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TOYO SEIKAN GROUP HOLDINGS LTD (JP)

C12Q1/6874; C12M1/00; C12N15/09

JP2006518189A	2006-08-10
JP2016183916A	2016-10-20
JP2009229210A	2009-10-08

IKUTOMI Shigekazu (JP)

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