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Title:
METHOD FOR ESTIMATING MICROBIAL DENSITY AND MICROARRAY FOR ESTIMATING MICROBIAL DENSITY
Document Type and Number:
WIPO Patent Application WO/2019/044126
Kind Code:
A1
Abstract:
[Problem] To enable the estimation of the density of microorganisms in an environment. [Solution] A method for estimating the density of microorganisms, said method comprising using the after-mentioned probes a and b, measuring signal values which indicate the hybridization levels of the probes with a polynucleotide derived from the target microorganisms, and determining to which of the after-mentioned patterns 1 to 3 the microbial density belongs on the basis of the signal values and a threshold. Probe a: A probe which comprises a base sequence specifically hybridizable with the polynucleotide and shows a signal value being equal to or higher than the threshold at density α. Probe(s) b: One or more probes which comprise a base sequence specifically hybridizable with the polynucleotide and show a signal value being equal to or higher than the threshold at density β that is lower than density α. Pattern 1: When both of the signal values of probes a and b are equal to or higher than the threshold, the density is equal to or higher than α. Pattern 2: When the signal value of probe a is lower than the threshold and the signal value of probe b is equal to or higher than the threshold, the density is equal to or higher than β and lower than α. Pattern 3: When both of the signal values of probes a and b are lower than the threshold, the density is lower than β.

Inventors:
OTSU Takayoshi (22-4, Okazawa-cho, Hodogaya-ku, Yokohama-sh, Kanagawa 62, 〒2400062, JP)
DOUSHI Hiroki (22-4, Okazawa-cho, Hodogaya-ku, Yokohama-sh, Kanagawa 62, 〒2400062, JP)
ISSHIKI Atsunori (22-4, Okazawa-cho, Hodogaya-ku, Yokohama-sh, Kanagawa 62, 〒2400062, JP)
Application Number:
JP2018/023392
Publication Date:
March 07, 2019
Filing Date:
June 20, 2018
Export Citation:
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Assignee:
TOYO SEIKAN GROUP HOLDINGS, LTD. (18-1, Higashi-Gotanda 2-chome Shinagawa-k, Tokyo 27, 〒1418627, JP)
International Classes:
C12Q1/6874; C12M1/00; C12N15/09
Foreign References:
JP2006518189A2006-08-10
JP2016183916A2016-10-20
JP2009229210A2009-10-08
Attorney, Agent or Firm:
IKUTOMI Shigekazu (Utsumi 2nd Bldg. 5th Floor, 34-1 Okusawa 6-chome, Setagaya-k, Tokyo 83, 〒1580083, JP)
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