Title:
METHOD FOR EVALUATING ANTIOXIDATIVE CAPACITY USING ELECTRON SPIN RESONANCE DEVICE, AND THE DEVICE
Document Type and Number:
WIPO Patent Application WO/2011/128987
Kind Code:
A1
Abstract:
Provided is a method for evaluating antioxidative capacity using an electron spin resonance device, whereby the antioxidative capacity of a living organism can be evaluated without damaging the living organism such as a disease organism or cells. Also provided is said device.
The method for evaluating the antioxidative capacity of a living organism comprises: a step for administering a reagent containing a free radical to the living organism; a first measurement step for measuring the free radical in a tissue fluid, which is collected from the living organism, using an electron spin resonance device; a step for re-radicalizing the tissue fluid having been collected from the living organism; a second measurement step for measuring the free radical in the re-radicalized tissue fluid using the electron spin resonance device; and a step for calculating the first and second free radical amounts from the measurement results obtained in the first and second measurement steps and comparing these amounts.
Inventors:
UEDA YUTO (JP)
NAKAJIMA AKIRA (JP)
OIKAWA TANEAKI (JP)
NAKAJIMA AKIRA (JP)
OIKAWA TANEAKI (JP)
Application Number:
PCT/JP2010/056641
Publication Date:
October 20, 2011
Filing Date:
April 14, 2010
Export Citation:
Assignee:
INST CREATIVE BIOTECHNOLOGY LTD (JP)
UEDA YUTO (JP)
NAKAJIMA AKIRA (JP)
OIKAWA TANEAKI (JP)
UEDA YUTO (JP)
NAKAJIMA AKIRA (JP)
OIKAWA TANEAKI (JP)
International Classes:
G01N24/10
Foreign References:
| JPS62126337A | 1987-06-08 |
Other References:
YUTO UEDA ET AL.: "In vivo activation of N-methyl-D-aspartate receptors generates free radicals and reduces antioxidant ability in the rat hippocampus: Experimental protocol of in vivo ESR spectroscopy and microdialysis for redox status evaluation", BRAIN RESEARCH, vol. 1178, 31 October 2007 (2007-10-31), pages 20 - 27
YUTO UEDA ET AL.: "Microdialysis-ESR Ho o Mochiita Chusu Byotai Model no Nonai Kosanka No Hyoka", THE SOCIETY OF ELECTRON SPIN SCIENCE AND TECHNOLOGY NENKAI KOEN YOSHISHU, vol. 45, 14 November 2006 (2006-11-14), pages 8 - 9
YUSHIN FUJIMURA ET AL.: "Nosotchu Rat, Seijo Rat no Nonai Free Radical no Dotai", NAGOYA CITY PUBLIC RESEARCH INSTITUTE HO, no. 44, 30 November 1998 (1998-11-30), pages 1 - 5
YUTO UEDA ET AL.: "Microdialysis-ESR Ho o Mochiita Chusu Byotai Model no Nonai Kosanka No Hyoka", THE SOCIETY OF ELECTRON SPIN SCIENCE AND TECHNOLOGY NENKAI KOEN YOSHISHU, vol. 45, 14 November 2006 (2006-11-14), pages 8 - 9
YUSHIN FUJIMURA ET AL.: "Nosotchu Rat, Seijo Rat no Nonai Free Radical no Dotai", NAGOYA CITY PUBLIC RESEARCH INSTITUTE HO, no. 44, 30 November 1998 (1998-11-30), pages 1 - 5
Attorney, Agent or Firm:
KATAYOSE KYOZO (JP)
Kyozo Katayose (JP)
Kyozo Katayose (JP)
Download PDF:
Claims: