Title:
METHOD FOR EVALUATING EMBRYOID BODY
Document Type and Number:
WIPO Patent Application WO/2019/163802
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: a method for evaluating differential characteristic of an embryoid body obtainable from cultivation of pluripotent liver cells; a method for screening an embryoid body obtainable from cultivation of pluripotent liver cells; a method for modifying myocardiac cells from pluripotent liver cells; a cell population comprising an embryoid body obtained by the screening method and/or differentiation-induced cells obtained by differentiation-inducing the embryoid body; a pharmaceutical composition comprising the embryoid body and/or the cell population; and a method for producing the pharmaceutical composition. The purpose is achieved by a method for evaluating differential characteristic of an embryoid body obtainable from cultivation of pluripotent liver cells, the method comprising a step for measuring one or more morphological characteristics of the embryoid body.
Inventors:
MIYAGAWA SHIGERU (JP)
SAWA YOSHIKI (JP)
NAGAI TARO (JP)
YAMAKI KAZUMASA (JP)
OYAMA KENJI (JP)
OHASHI FUMIYA (JP)
SAWA YOSHIKI (JP)
NAGAI TARO (JP)
YAMAKI KAZUMASA (JP)
OYAMA KENJI (JP)
OHASHI FUMIYA (JP)
Application Number:
PCT/JP2019/006223
Publication Date:
August 29, 2019
Filing Date:
February 20, 2019
Export Citation:
Assignee:
UNIV OSAKA (JP)
DAINIPPON PRINTING CO LTD (JP)
TERUMO CORP (JP)
DAINIPPON PRINTING CO LTD (JP)
TERUMO CORP (JP)
International Classes:
C12Q1/02; A61K35/34; A61P9/10; C12N5/077; C12N5/10
Other References:
SASAKI D. ET AL.: "Mass preparation of size-controlled mouse embryonic stem cell aggregates and induction of cardiac differentiation by cell patterning method", BIOMATERIALS, vol. 30, no. 26, 30 September 2009 (2009-09-30), pages 4384 - 4389, XP026337764, doi:10.1016/j.biomaterials.2009.05.003
HWANG Y. S. ET AL.: "Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11", PROC. NATL. ACAD. SCI., vol. 106, no. 40, 6 October 2009 (2009-10-06), pages 16978 - 16983, XP055067093, doi:10.1073/pnas.0905550106
MOHR J. C. ET AL.: "The microwell control of embryoid body size in order to regulate cardiac differentiation of human embryonic stem cells", BIOMATERIALS, vol. 31, no. 7, March 2010 (2010-03-01), pages 1885 - 1893, XP026827550
HWANG Y. S. ET AL.: "Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11", PROC. NATL. ACAD. SCI., vol. 106, no. 40, 6 October 2009 (2009-10-06), pages 16978 - 16983, XP055067093, doi:10.1073/pnas.0905550106
MOHR J. C. ET AL.: "The microwell control of embryoid body size in order to regulate cardiac differentiation of human embryonic stem cells", BIOMATERIALS, vol. 31, no. 7, March 2010 (2010-03-01), pages 1885 - 1893, XP026827550
Attorney, Agent or Firm:
KUZUWA, Kiyoshi (JP)
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