Title:
METHOD OF EVALUATING PHOSPHATASE INHIBITORS
Document Type and Number:
WIPO Patent Application WO/2003/038118
Kind Code:
A1
Abstract:
OVARC1000473 (SEQ ID NO:1) and NT2RM1000377 (SEQ ID NO:3) are identified as clones inhibiting the activation of CREB by folskolin and an evaluation method using these genes and&sol or proteins encoded by these genes is provided. It is also found out that these proteins have an effect of enhancing cytotoxicity. Compounds which can be screened based on the above evaluation method are useful as CREB dephosphorylation inhibitors, cytotoxicity enhancement inhibitors and preventives and remedies for retention defect and&sol or neurodegenerative diseases.
Inventors:
MORITA MASAHIKO (JP)
ARAKAWA HIROYUKI (JP)
YAMAZAKI MAYAKO (JP)
SATOH SUSUMU (JP)
NISHIMURA SHINTARO (JP)
KITA YASUHIRO (JP)
YAMAZAKI TAKAO (JP)
ARAKAWA HIROYUKI (JP)
YAMAZAKI MAYAKO (JP)
SATOH SUSUMU (JP)
NISHIMURA SHINTARO (JP)
KITA YASUHIRO (JP)
YAMAZAKI TAKAO (JP)
Application Number:
PCT/JP2002/011365
Publication Date:
May 08, 2003
Filing Date:
October 31, 2002
Export Citation:
Assignee:
FUJISAWA PHARMACEUTICAL CO (JP)
MORITA MASAHIKO (JP)
ARAKAWA HIROYUKI (JP)
YAMAZAKI MAYAKO (JP)
SATOH SUSUMU (JP)
NISHIMURA SHINTARO (JP)
KITA YASUHIRO (JP)
YAMAZAKI TAKAO (JP)
MORITA MASAHIKO (JP)
ARAKAWA HIROYUKI (JP)
YAMAZAKI MAYAKO (JP)
SATOH SUSUMU (JP)
NISHIMURA SHINTARO (JP)
KITA YASUHIRO (JP)
YAMAZAKI TAKAO (JP)
International Classes:
A61K31/713; A61P25/00; C07K14/47; C12Q1/42; (IPC1-7): C12Q1/48; C12N15/00; A61K31/713; A61K39/395; G01N33/50; G01N33/15; A61P25/00
Domestic Patent References:
| WO2002020732A2 | 2002-03-14 |
Foreign References:
| EP1074617A2 | 2001-02-07 | |||
| EP1074617A2 | 2001-02-07 |
Other References:
HAGIWARA M. ET AL.: "Transcriptional attenuation following cAMP induction requires PP-1-mediated dephosphorylation of CREB", CELL, vol. 70, no. 1, July 1992 (1992-07-01), pages 105 - 113, XP002935359
BITO H. ET AL.: "CREB phosphorylation and dephosphorylation: a Ca(2+)- and stimulus duration-dependent switch for hippocampal gene expression", CELL, vol. 87, no. 7, December 1996 (1996-12-01), pages 1203 - 1214, XP002964775
REUSCH JANE E.B. ET AL.: "Insulin inhibits dephosphorylation of adenosine 3',5'-monophosphate response element-binding protein/activating transcription factor-1: effect on nuclear phosphoserine phosphatase-2a", ENDOCRINOLOGY, vol. 135, no. 6, 1994, pages 2418 - 2422, XP002964774
WADZINSKI B.E. ET AL.: "Nuclear protein phosphatase 2A dephosphorylation protein kinase A-phosphorylated CREB and regulates CREB transcriptional stimulation", MOLECULAR AND CELLULAR BIOLOGY, vol. 13, no. 5, 1993, pages 2822 - 2834, XP002964773
WHEAT W.H. ET AL.: "Simian virus 40 small tumor antigen inhibits dephosphorylation of protein kinase A-phosphorylated CREB and regulates CREB transcriptional stimulation", MOLECULAR AND CELLULAR BIOLOGY, vol. 14, no. 9, 1994, pages 5881 - 5890, XP002904785
SHAYWITZ, A. J. ET AL., ANNU. REV. BIOCHEM., vol. 68, 1999, pages 821
BEITMER-JOHNSON, D. ET AL., J. BIOL. CHEM., vol. 273, 1998, pages 19834 - 19839
DEISSEROTH, K. ET AL., NEURON, vol. 16, 1996, pages 89 - 101
HARDINGHAM, G. E. ET AL., NAT. NEUROSCI., vol. 4, 2001, pages 261 - 267
SILVA, A. J. ET AL., ANNU. REV. NEUROSCI., vol. 21, 1998, pages 127
YAMAMOTO-SASAKI, M. ET AL., BRAIN RES., vol. 824, 1999, pages 300 - 303
TANAKA, K. ET AL., BRAIN RES., vol. 818, 1999, pages 520 - 526
RICCO, A. ET AL., SCIENCE, vol. 286, 1999, pages 2358 - 2361
NUCIFORA JR., F. C. ET AL., SCIENCE, vol. 291, 2001, pages 2423 - 2428
HAGIWARA, M. ET AL., CELL, vol. 70, 1992, pages 105 - 113
COHEN, P., ANNU. REV. BIOCHEM., vol. 58, 1989, pages 453 - 508
HAGIWARA ET AL., CELL, vol. 70, 1992, pages 105 - 113
KEYSE S. M., BIOCHEM. BIOPHYS. ACTA, vol. 1265, no. 2-3, 16 March 1995 (1995-03-16), pages 152 - 60
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
ALTSHUL ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
T. MANIATIS ET AL.: "Molecular Cloning", 1989, COLD SPRING HARBOR LABORATORY
T. MANIATIS ET AL.: "Molecular Cloning", 2001, COLD SPRING HARBOR LABORATORY
D. M. GLOVER: "DNA Cloning", 1985, IRL PRESS
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
BEITNER-JOHNSON D. ET AL., J. BIOL. CHEM., vol. 273, no. 31, 31 July 1998 (1998-07-31), pages 19834 - 9
STEIN: "Physicochemical properties of phosphorothioate oligodeoxynucleotides", NUCLEIC ACIDS RES., vol. 16, 1988, pages 3209 - 3221
See also references of EP 1441034A4
BITO H. ET AL.: "CREB phosphorylation and dephosphorylation: a Ca(2+)- and stimulus duration-dependent switch for hippocampal gene expression", CELL, vol. 87, no. 7, December 1996 (1996-12-01), pages 1203 - 1214, XP002964775
REUSCH JANE E.B. ET AL.: "Insulin inhibits dephosphorylation of adenosine 3',5'-monophosphate response element-binding protein/activating transcription factor-1: effect on nuclear phosphoserine phosphatase-2a", ENDOCRINOLOGY, vol. 135, no. 6, 1994, pages 2418 - 2422, XP002964774
WADZINSKI B.E. ET AL.: "Nuclear protein phosphatase 2A dephosphorylation protein kinase A-phosphorylated CREB and regulates CREB transcriptional stimulation", MOLECULAR AND CELLULAR BIOLOGY, vol. 13, no. 5, 1993, pages 2822 - 2834, XP002964773
WHEAT W.H. ET AL.: "Simian virus 40 small tumor antigen inhibits dephosphorylation of protein kinase A-phosphorylated CREB and regulates CREB transcriptional stimulation", MOLECULAR AND CELLULAR BIOLOGY, vol. 14, no. 9, 1994, pages 5881 - 5890, XP002904785
SHAYWITZ, A. J. ET AL., ANNU. REV. BIOCHEM., vol. 68, 1999, pages 821
BEITMER-JOHNSON, D. ET AL., J. BIOL. CHEM., vol. 273, 1998, pages 19834 - 19839
DEISSEROTH, K. ET AL., NEURON, vol. 16, 1996, pages 89 - 101
HARDINGHAM, G. E. ET AL., NAT. NEUROSCI., vol. 4, 2001, pages 261 - 267
SILVA, A. J. ET AL., ANNU. REV. NEUROSCI., vol. 21, 1998, pages 127
YAMAMOTO-SASAKI, M. ET AL., BRAIN RES., vol. 824, 1999, pages 300 - 303
TANAKA, K. ET AL., BRAIN RES., vol. 818, 1999, pages 520 - 526
RICCO, A. ET AL., SCIENCE, vol. 286, 1999, pages 2358 - 2361
NUCIFORA JR., F. C. ET AL., SCIENCE, vol. 291, 2001, pages 2423 - 2428
HAGIWARA, M. ET AL., CELL, vol. 70, 1992, pages 105 - 113
COHEN, P., ANNU. REV. BIOCHEM., vol. 58, 1989, pages 453 - 508
HAGIWARA ET AL., CELL, vol. 70, 1992, pages 105 - 113
KEYSE S. M., BIOCHEM. BIOPHYS. ACTA, vol. 1265, no. 2-3, 16 March 1995 (1995-03-16), pages 152 - 60
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
ALTSHUL ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
T. MANIATIS ET AL.: "Molecular Cloning", 1989, COLD SPRING HARBOR LABORATORY
T. MANIATIS ET AL.: "Molecular Cloning", 2001, COLD SPRING HARBOR LABORATORY
D. M. GLOVER: "DNA Cloning", 1985, IRL PRESS
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
BEITNER-JOHNSON D. ET AL., J. BIOL. CHEM., vol. 273, no. 31, 31 July 1998 (1998-07-31), pages 19834 - 9
STEIN: "Physicochemical properties of phosphorothioate oligodeoxynucleotides", NUCLEIC ACIDS RES., vol. 16, 1988, pages 3209 - 3221
See also references of EP 1441034A4
Attorney, Agent or Firm:
Shimizu, Hatsushi (1-1-1 Oroshi-mach, Tsuchiura-shi Ibaraki, JP)
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