Title:
METHOD FOR EVALUATING SPECIFIC INCORPORATION OF D-GLUCOSE INTO CELLS
Document Type and Number:
WIPO Patent Application WO/2010/016587
Kind Code:
A1
Abstract:
Disclosed is a method for accurately evaluating the specific incorporation of D-glucose into cells. The method is characterized by: contacting each of a D-glucose derivative that can be incorporated into cells specifically and has a fluorescent chromophore in the molecule and an L-glucose derivative that has a fluorescent chromophore in the molecule with cells of a cell species to be evaluated; comparing the fluorescence emitted from the D-glucose derivative that can be incorporated into cells specifically and has the fluorescent chromophore in the molecule with the fluorescence emitted from the L-glucose derivative that has the fluorescent chromophore in the molecule; and determining the difference between the above-mentioned fluorescence intensities as the specific incorporation of D-glucose into cells relative to that of L-glucose.
Inventors:
YAMADA KATSUYA (JP)
MATSUOKA HIDEAKI (JP)
TESHIMA TADASHI (JP)
YAMAMOTO TOSHIHIRO (JP)
MATSUOKA HIDEAKI (JP)
TESHIMA TADASHI (JP)
YAMAMOTO TOSHIHIRO (JP)
Application Number:
PCT/JP2009/064053
Publication Date:
February 11, 2010
Filing Date:
August 07, 2009
Export Citation:
Assignee:
UNIV HIROSAKI (JP)
UNIV TOKYO NAT UNIV CORP (JP)
YAMADA KATSUYA (JP)
MATSUOKA HIDEAKI (JP)
TESHIMA TADASHI (JP)
YAMAMOTO TOSHIHIRO (JP)
UNIV TOKYO NAT UNIV CORP (JP)
YAMADA KATSUYA (JP)
MATSUOKA HIDEAKI (JP)
TESHIMA TADASHI (JP)
YAMAMOTO TOSHIHIRO (JP)
International Classes:
C12Q1/54; C07H1/00; C07H5/06; C07H13/00; C07H17/00; C12Q1/04
Other References:
YAMADA, K. ET AL.: "A real-time method of imaging glucose uptake in single, living mammalian cells.", NAT. PROTOC., vol. 2, no. 3, 2007, pages 753 - 762, XP008142453
YAMADA, K. ET AL.: "Application of highly pure fluorescent glucose derivative 2-NBDG into neurons.", NEUROSCIENCE RESEARCH, vol. 61, no. 1, July 2008 (2008-07-01), pages S138, XP008142399
CONDE, C. ET AL.: "An Hg-sensitive channel mediates the diffusional component of glucose transport in olive cells.", BIOCHIM.BIOPHYS. ACTA., vol. 1768, no. 11, 2007, pages 2801 - 2811, XP022338998
LEVI, J. ET AL.: "Fluorescent fructose derivatives for imaging breast cancer cells.", BIOCONJUG.CHEM., vol. 18, no. 3, 2007, pages 628 - 634, XP008142401
SPEIZER, L. ET AL.: "Asymmetric transport of a fluorescent glucose analogue by human erythrocytes.", BIOCHIM.BIOPHYS.ACTA, vol. 815, no. 1, 1985, pages 75 - 84, XP023364434
HALAMA, J.R. ET AL.: "Validation of 3-deoxy-3-fluoro-D-glucose as a glucose transport analogue in rat heart.", AM. J. PHYSIOL., vol. 247, no. 5.PT2., 1984, pages H754 - H759, XP008142384
YAMAMOTO, T. ET AL.: "Synthesis of 2-NBDLG, a fluorescent derivative of 1-glucosamine; the antipode of d-glucose tracer 2-NBDG.", TETRAHEDRON LETTERS, vol. 49, no. 48, 24 November 2008 (2008-11-24), pages 6876 - 6878, XP025533080
See also references of EP 2325327A4
YAMADA K. ET AL., J. BIOL. CHEM., vol. 275, 2000, pages 22278 - 22283
YAMADA K. ET AL., NAT. PROTOC., vol. 2, 2007, pages 753 - 762
SPEIZER L. ET AL., BIOCHIM. BIOPHYS. ACTA, vol. 815, 1985, pages 75 - 84
YAMADA, K. ET AL.: "Application of highly pure fluorescent glucose derivative 2-NBDG into neurons.", NEUROSCIENCE RESEARCH, vol. 61, no. 1, July 2008 (2008-07-01), pages S138, XP008142399
CONDE, C. ET AL.: "An Hg-sensitive channel mediates the diffusional component of glucose transport in olive cells.", BIOCHIM.BIOPHYS. ACTA., vol. 1768, no. 11, 2007, pages 2801 - 2811, XP022338998
LEVI, J. ET AL.: "Fluorescent fructose derivatives for imaging breast cancer cells.", BIOCONJUG.CHEM., vol. 18, no. 3, 2007, pages 628 - 634, XP008142401
SPEIZER, L. ET AL.: "Asymmetric transport of a fluorescent glucose analogue by human erythrocytes.", BIOCHIM.BIOPHYS.ACTA, vol. 815, no. 1, 1985, pages 75 - 84, XP023364434
HALAMA, J.R. ET AL.: "Validation of 3-deoxy-3-fluoro-D-glucose as a glucose transport analogue in rat heart.", AM. J. PHYSIOL., vol. 247, no. 5.PT2., 1984, pages H754 - H759, XP008142384
YAMAMOTO, T. ET AL.: "Synthesis of 2-NBDLG, a fluorescent derivative of 1-glucosamine; the antipode of d-glucose tracer 2-NBDG.", TETRAHEDRON LETTERS, vol. 49, no. 48, 24 November 2008 (2008-11-24), pages 6876 - 6878, XP025533080
See also references of EP 2325327A4
YAMADA K. ET AL., J. BIOL. CHEM., vol. 275, 2000, pages 22278 - 22283
YAMADA K. ET AL., NAT. PROTOC., vol. 2, 2007, pages 753 - 762
SPEIZER L. ET AL., BIOCHIM. BIOPHYS. ACTA, vol. 815, 1985, pages 75 - 84
Attorney, Agent or Firm:
TSUJITA Takashi et al. (JP)
Yukifumi Tsujita (JP)
Yukifumi Tsujita (JP)
Download PDF: