Title:
METHOD FOR EXPRESSING AND ISOLATING SOLUBLE PROTEIN OF CRM197
Document Type and Number:
WIPO Patent Application WO/2018/143648
Kind Code:
A2
Abstract:
Disclosed is a method for producing a soluble CRM197 protein at high yield by excessively expressing the protein in the cytoplasm of E. coli. The present invention provides a method for producing a recombinant CRM197 protein soluble in the cytoplasm, the method comprising a step of culturing recombinant E. coli anchoring an expression plasmid carrying a gene sequence coding for a recombinant CRM197 protein under a condition suitable for expression of the recombinant CRM197 protein which has a histidine-tag attached to the C-terminus of CRM197 and a maltose-binding protein (MBP) attached to the N-terminus of CRM197.
Inventors:
LEE HYEON CHEOL (KR)
KOO BONG SEONG (KR)
SEO HYOUNG JONG (KR)
KIM JIN SOOK (KR)
PARK AH REUM (KR)
JANG SEUNG WON (KR)
KOO BONG SEONG (KR)
SEO HYOUNG JONG (KR)
KIM JIN SOOK (KR)
PARK AH REUM (KR)
JANG SEUNG WON (KR)
Application Number:
PCT/KR2018/001293
Publication Date:
August 09, 2018
Filing Date:
January 30, 2018
Export Citation:
Assignee:
FORBIOKOREA CO LTD (KR)
International Classes:
C07K14/34; C07K1/22; C12N15/70
Attorney, Agent or Firm:
PARK, Sang Hoon (KR)
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