Title:
METHOD FOR EXPRESSING AND PREPARING RECOMBINANT RETEPLASE BY USING GENETICALLY ENGINEERED RICE
Document Type and Number:
WIPO Patent Application WO/2023/274091
Kind Code:
A1
Abstract:
A recombinant reteplase gene optimized by a rice codon, a related vector, a method for preparing a genetically engineered rice seed, and a method for separating and purifying recombinant reteplase. Genetically engineered rice is processed and crushed, and is then mixed with an extraction buffer, and extraction and filtering are performed to obtain a crude extract containing recombinant reteplase; and separation and purification steps sequentially comprise: 1) performing cation exchange chromatography on the crude extract containing the recombinant reteplase to obtain a primary product I; 2) performing hydrophobic chromatography on the primary product I to obtain an intermediate product II; and 3) performing benzamidine affinity chromatography on the intermediate product II to obtain purified recombinant reteplase.
Inventors:
YANG DAICHANG (CN)
YU WENHUI (CN)
YU WENHUI (CN)
Application Number:
PCT/CN2022/101339
Publication Date:
January 05, 2023
Filing Date:
June 24, 2022
Export Citation:
Assignee:
WUHAN HEALTHGEN BIOTECHNOLOGY CORP (CN)
International Classes:
C12N15/84; C07K1/16; C07K1/20; C07K14/745; C12N9/64
Domestic Patent References:
| WO2020078254A1 | 2020-04-23 | |||
| WO2020119609A1 | 2020-06-18 | |||
| WO2017052329A1 | 2017-03-30 |
Foreign References:
| CN107083399A | 2017-08-22 | |||
| CN112266415A | 2021-01-26 | |||
| CN101195824A | 2008-06-11 | |||
| CN1414094A | 2003-04-30 | |||
| CN113337493A | 2021-09-03 |
Attorney, Agent or Firm:
LECOME INTELLECTUAL PROPERTY AGENT LTD. (CN)
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