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Title:
METHOD FOR EXTRACTING GYMNADENIA CONOPSEA AND RELATED GYMNADENIA CONOPSEA EXTRACT
Document Type and Number:
WIPO Patent Application WO/2020/207313
Kind Code:
A1
Abstract:
Disclosed is a method for extracting Gymnadenia conopsea, which comprises: (1) soaking tuberous roots of Gymnadenia conopsea in water, fully soaking the tuberous roots until the tuberous roots of Gymnadenia conopsea are sampled and observed as having no white cores; (2) performing wet superfine grinding on the tuberous roots of Gymnadenia conopsea to obtain a dispersion slurry; (3) adding additional water into the dispersion slurry to obtain a diluted dispersion liquid, heating same to make the temperature rise, then adding a neutral protease, and then performing extraction through circulation and homogenization by means of a homogenizing pump to obtain an extract liquid; (4) performing heat preservation and enzyme inactivation; (5) performing crude filtration to obtain a crude filtrate; and (6) performing fine filtration to obtain a refined filtrate. Also provided is a related Gymnadenia conopsea extract. The extracted Gymnadenia conopsea extract is safe and has unique application value in terms of skin care, wound repair, wrinkle resistance, sun protection, oxidation resistance, allergy resistance, skin moisture retention, etc., has a good application prospect in the field of cosmetics, and is ingenious in terms of process design, simple and convenient to operate and low in cost.

Inventors:
LIU XING (CN)
WANG JIBIN (CN)
CHEN HAIHUA (CN)
DING ZUOKUN (CN)
WANG CHUANHAO (CN)
WANG JIWEN (CN)
Application Number:
PCT/CN2020/082792
Publication Date:
October 15, 2020
Filing Date:
April 01, 2020
Export Citation:
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Assignee:
SHANGHAI HOPE TEC BIOTECHNOLOGY INC (CN)
International Classes:
A61K8/9794; A61Q17/04; A61Q19/00; A61Q19/08
Foreign References:
CN109939057A2019-06-28
CN101647958A2010-02-17
Other References:
YANG, MINGMING: "Study on Polysaccharides Characteristics from Gymnadenia Conopsea R.Br.", CHINA MASTER’S THESES FULL-TEXT DATABASE_MEDICINE AND HEALTH TECHNOLOGY, no. 03, 15 March 2013 (2013-03-15), pages 1 - 73, XP055742340
ZHANG, XIAOHONG ET AL.: "The Structural Analysis of Gymnadenia Conopsea Polysaccharide", JOURNAL OF INNER MONGOLIA UNIVERSITY (NATURAL SCIENCE EDITION, vol. 36, no. 02, 31 March 2005 (2005-03-31), pages 1 - 4, XP055742346
SUN, PINGDENG: "Study on the Extraction of Polyasccharides from Gymnadenia conopsea R.Br.", FOOD RESEARCH AND DEVELOPMENT, vol. 31, no. 03, 31 March 2010 (2010-03-31), pages 1 - 4, XP055742355
LIN, PENG-CHENG ET AL.,: "Characterization and comparison of bioactive polysaccharides from the tubers of Gymnadenia conopsea,", FOOD HYDROCOLLOIDS,, vol. 43, 28 May 2014 (2014-05-28), pages 199 - 206, XP055742465, DOI: 20200602101925
Attorney, Agent or Firm:
BEIJING LONGAN LAW FIRM (CN)
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