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Title:
A METHOD FOR EXTRACTING PROTEIN FROM PECTIN RICH PLANT MATERIAL
Document Type and Number:
WIPO Patent Application WO/2012/134262
Kind Code:
A3
Abstract:
The present invention relates to methods for extracting protein from plant material which is characterized by a high content of interfering compounds, specifically abundant polysaccharides like pectin. The inventive method applies a pectolytic treatment in preparation of the plant material sample prior to protein extraction. This optimized method results in high quality protein extracts compatible with further sensitive proteomic analyses specifically methods like two dimensional gel electrophoresis or mass spectrometry. The present invention further relates to the protein extracts obtained by the inventive method and the use of the inventive method in the preparation of protein samples. Also a protein extraction kit is disclosed for conducting the inventive method, as well as the use of the protein extraction kit in protein analysis and proteomics.

Inventors:
HAJI AHMAD ZALINAH (MY)
RASTEGARI ELHAM (MY)
SPENCER DAVID FARRINGDON (CA)
ISMAIL MAZNAH (MY)
Application Number:
MY2012/000044
Publication Date:
December 27, 2012
Filing Date:
March 07, 2012
Export Citation:
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Assignee:
UNIVERSITI PUTRA MALAYSIA U P M (MY)
HAJI AHMAD ZALINAH (MY)
RASTEGARI ELHAM (MY)
SPENCER DAVID FARRINGDON (CA)
ISMAIL MAZNAH (MY)
International Classes:
C07K14/415; C07K1/12; C12P21/06; G01N33/68
Foreign References:
US6313273B12001-11-06
US5830738A1998-11-03
Other References:
JUNG ET AL.: "Functionality of soy protein produced by enzyme-assisted extraction", JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY, vol. 83, no. 1, January 2006 (2006-01-01), pages 71 - 78
ZHOU ET AL.: "FT-IR methodology for quality control of arabinogalactan protein (AGP) extracted from green tea (Camellia sinensis)", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 57, 20 May 2009 (2009-05-20), pages 5121 - 5128
Attorney, Agent or Firm:
KAUR, Sushil (D-6Sunway PJ,51A,SS9A/19,Section 51, Petaling Jaya Selangor, MY)
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