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Title:
METHOD FOR EXTRACTING AND PURIFYING L-ERGOTHIONEINE
Document Type and Number:
WIPO Patent Application WO/2015/103974
Kind Code:
A1
Abstract:
A method for extracting and purifying L-ergothioneine comprises the following steps: (1) soaking and extracting a mushroom mycelium fermentation liquid by using hot water, so as to obtain a mushroom and L-ergothioneine water solution; (2) performing ultrafiltration of the mushroom and L-ergothioneine water solution by using an ultrafiltration membrane with a molecular weight cut-off of 1kDa to 30kDa, and collecting an ultrafiltration liquid; and (3) performing chromatographic purification of the ultrafiltration permeation liquid, the chromatographic purification conditions being: a chromatographic filler being an HILIC filler, a mobile phase forming acetonitrile-water with a volume ratio of 80: 20 to 88: 12, and a quantity of feed of L-ergothioneine being 0.01g to 0.323 per 100g.

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Inventors:
JIANG WENXIA (CN)
LIU QI (CN)
WANG HONGYU (CN)
LI YUNHUA (CN)
LIU WEI (CN)
MEI BAOLIANG (CN)
ZHANG WEIYA (CN)
Application Number:
PCT/CN2015/070246
Publication Date:
July 16, 2015
Filing Date:
January 07, 2015
Export Citation:
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Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C07D233/84; C12P13/04; C12R1/645
Foreign References:
CN103734022A2014-04-23
CN103743825A2014-04-23
CN103184246A2013-07-03
JP2008110988A2008-05-15
Attorney, Agent or Firm:
SHANGCHENG & PARTNERS (CN)
北京尚诚知识产权代理有限公司 (CN)
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